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...could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by introducing... the culture, so bacteria must be grown in a turbidostat. Only mutate particular types of nucleotides...

... their low leakiness. Inducibility level How much do you want to induce transcription of your target gene...

...students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities...

...questions regarding topics such as informed consent (How do we prevent stem cell lines from becoming the new ...

...majority of restriction enzymes commonly used in the lab do not have recognition sites that could overlap with...

...about the types of experiments you're planning on doing and read the eBook or browse our CRISPR resources...

...sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so off-target...

...) antigens. Unfortunately, antibodies frequently do not work as assumed (Ayoubi et al., 2023). Additionally...may not be available to every scientist looking to do a flow cytometry experiment. But validation is critical...

...domains—including the CTD and the entire REC lobe—do not structurally resemble any other known proteins...check for sequence matches; a perfect guide RNA will do you no good if there is no adjacent PAM to anchor...

...we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment? The three years...

...communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural cells, ion-based...

...interest into a bacterial cell or a host’s genome. To do this scientists commonly use phagemids, a DNA cloning...

...spatio-temporal dynamics of apoptosis in living animals. To do this they modified a different self-assembling split...

...go to grad school. But, I didn’t know what else to do. Fortunately, the head of software product management...

...broader community, and preprints are a great way to do that. We are happy to receive deposits of plasmids...

...resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: ...

...and are slightly different in sequence. Both LTRs do share common repeat regions in addition to their ...virion assembly and particle release from late endosomes when AAV is trafficked. Rep and cap mediate AAV...

...produced dsRNA rarely reach this threshold and thus do not typically activate an inflammatory response. ...

...strength of staining and background noise. If you do use different lots of your polyclonal antibody, you...

...sensors in terms of response and brightness. They also do not spectrally separate from green sensors and blue-light-activated.... The dosing required to achieve these efficiencies was also between 1013 - 1014 vg/kg, a dosage comparable...