We narrowed to 770 results for: Dos;
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TypeBlog Post...chromosomal loci in human cells (Ma et al., 2015). To do so, they fused a different fluorescent protein to...
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Targeted Mutagenesis with EvolvR
TypeBlog Post...could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by introducing... the culture, so bacteria must be grown in a turbidostat. Only mutate particular types of nucleotides... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post...monoclonal antibody. But what does that mean, and how do monoclonal antibodies differ from other types of ... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog Post...You can find more information about us and what we do in our Frequently Asked Questions. We also invite... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post... disillusioned with the gulf between what I was doing in the lab and the interactions I was having outside... -
A Deep Dive into BioBuilder
TypeBlog Post...students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog Post...questions regarding topics such as informed consent (How do we prevent stem cell lines from becoming the new ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...majority of restriction enzymes commonly used in the lab do not have recognition sites that could overlap with... -
Components of CRISPR/Cas9
TypeBlog Post...about the types of experiments you're planning on doing and read the eBook or browse our CRISPR resources... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so off-target... -
Antibody Validation for Flow Cytometry
TypeBlog Post...) antigens. Unfortunately, antibodies frequently do not work as assumed (Ayoubi et al., 2023). Additionally...may not be available to every scientist looking to do a flow cytometry experiment. But validation is critical... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog Post...domains—including the CTD and the entire REC lobe—do not structurally resemble any other known proteins...check for sequence matches; a perfect guide RNA will do you no good if there is no adjacent PAM to anchor... -
Luciferase Plasmid Collection
TypeCollection...BRET biosensor for live imaging of mTOR signaling. Do you have suggestions for other plasmids that should... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post... mitigating the pH effects of dry ice storage. To do this, we stored samples on dry ice for 72 hours and... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...with the microscope that you will be using. If you do a head-to-head comparison right, it will be valuable... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...Central PMCID: PMC5120861. While there is much work to do, these early pilot studies have demonstrated technical... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...lentiviral inventory is based on CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post... posts are not comprehensive, so just because you do not see a name or gene on this list, that does not... -
Visualizing Protein Turnover In Situ
TypeBlog Post...blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover within a neuron? For... -
Viral Vectors 101: Viral Vector Elements
TypeBlog Post...and are slightly different in sequence. Both LTRs do share common repeat regions in addition to their ...virion assembly and particle release from late endosomes when AAV is trafficked. Rep and cap mediate AAV...