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We narrowed to 830 results for: EED

Showing: 561 - 580 of 830 results

  3. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...commercial strains designed to avoid them. Why do I need an expression strain? Protein expression from high-copy...number plasmids and powerful promoters will greatly exceed that of any native host protein, using up valuable...

  6. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...Lentiviral vectors can be produced easily, without the need for time-consuming purification processes, and are...delivered to cells, the viral life cycle initially proceeds as usual. Reverse transcription of the transgene...

  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...recruitment of katanin to that anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module...many tools available for controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and...

  9. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... antibody specificity and antigen structure. Proceedings of the National Academy of Sciences 76:3116–3120...nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences 76:4350–4354...

  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... avoid overlap with this optogenetic tool, they needed to create a calcium sensor with the following characteristics...could not be used as the excitation wavelengths needed would also activate the optogenetic accuators. ...

  11. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ... expression levels of your gene of interest, as needed. Controlling the expression levels of your gene...approach is that, since there is no selection, you will need to screen somewhere between one hundred and one ...

  13. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...reconstituting, choosing your colors depending on the needs of your experiment (Figure 3). And using self-labeling...TMP-tag or tetracysteine labeling dyes.  If reaction speed is a priority, SNAP-Tag or HaloTag have been shown...

  14. Typing CRISPR Systems

    Type
    Blog Post
    ...foreign DNA with unique properties. This led to a need to develop a classification system for the array... tools using a Cascade complex is tricky, as you need to ensure every unit of the complex expresses at...

  15. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...protein of interest in a single cell? Or perhaps, you need to analyze a specific subset of cells in a complex... being used together in an experiment, you would need a way to determine the relative contribution of ...
Showing: 561 - 580 of 830 results