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  1. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... avoid overlap with this optogenetic tool, they needed to create a calcium sensor with the following characteristics...could not be used as the excitation wavelengths needed would also activate the optogenetic accuators. ...

  2. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... antibody specificity and antigen structure. Proceedings of the National Academy of Sciences 76:3116–3120...nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences 76:4350–4354...

  3. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ... expression levels of your gene of interest, as needed. Controlling the expression levels of your gene...approach is that, since there is no selection, you will need to screen somewhere between one hundred and one ...

  4. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...reconstituting, choosing your colors depending on the needs of your experiment (Figure 3). And using self-labeling...TMP-tag or tetracysteine labeling dyes.  If reaction speed is a priority, SNAP-Tag or HaloTag have been shown...

  6. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...protein of interest in a single cell? Or perhaps, you need to analyze a specific subset of cells in a complex... being used together in an experiment, you would need a way to determine the relative contribution of ...

  7. Typing CRISPR Systems

    Type
    Blog Post
    ...foreign DNA with unique properties. This led to a need to develop a classification system for the array... tools using a Cascade complex is tricky, as you need to ensure every unit of the complex expresses at...

  15. 2022 Depositor Week

    Type
    Blog Post
    ...through our newly revamped Data Hub! The community needs more data-sharing heroes like you.)  ...

  17. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...From May 13 (today!) to May 17, the Addgene Twitter feed will be all about CRISPR. We’ll have a different...

  19. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...best-recycled lab supply ornament ever! & they totally succeed! WOW! #deckthelab Thank you to everyone who participated...

  20. We’re Going Viral This Week!

    Type
    Blog Post
    ...AddgeneGoesViral on Twitter Starting today, our Twitter feed will be full of viral vector information. Each day...
Showing: 561 - 580 of 816 results