We narrowed to 629 results for: EMB

1. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   ...the cell. Can be used for generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified...

2. AAVs in Retinal Gene Therapy

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   ...later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first ...

3. Transferable Skills Guide: Identifying Your Transferable Skills

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   ...commitments, respond to any issues raised by team members, and plan for the future of the team. All while...

4. Summer SciComm Series: Modes of Communication

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   ... limiting factor at multiple points. Before you embark on a project, do your best to understand what kind...

5. SciComm: Taking Science to Elementary and Middle Schools

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   ... Science Night, a chance for parents and other members of the community to share their expertise with ...

6. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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   ... materials expired? Did you pipette carefully? Remember to discard flow-through solutions and use clean...

7. Antibodies 101: Producing Recombinant Antibodies

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   ...system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (...

8. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   ...’t work for all bacteria.   Zeocin  Zeocin is a member of the bleomycin family of antibiotics, which induce...

9. CRISPR 101: Making a Knock-In Cell Line

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   ...out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a...

10. Cpf1: A New Tool for CRISPR Genome Editing

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    ...mammalian cells. Like Cas9 nucleases, Cpf1 family members contain a RuvC-like endonuclease domain, but they...

11. Plasmids 101: Plasmid Incompatibility

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    ...resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman et al., 2017)...

12. Neuronal labeling with Spaghetti Monster

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    ... Flying Spaghetti Monster; the smFP structure resembles images of the deity (see comparison image above...

13. Transferable Skills Guide: External Collaborations

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    ...feeling weird and started to be fun. I just had to remember to approach the relationship with the perspective...

14. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    ...al. 2016. “Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo.” Neuron...

15. New CRISPR Tools: Cas7-11 and PASTE

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    ...sensitive to toxicity from RNA editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development...

16. Antibodies 101: Immunoprecipitation

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    ...adjusted depending on the sample. It is important to remember that sample lysates will also contain proteases...

17. Viral Vectors 101: Pseudotyping

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    ...find tables and information on different viral membranes, tropism, and advantages and disadvantages: Table...

18. Deciphering the Mysteries of Behavior with Viral Vectors

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    ...noradrenergic excitability upon acute stress." The EMBO journal 37.21 (2018). PubMed PMID: 30209240. PubMed...

19. Save Time with Transient Plant Leaf Transformations

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    ...working vector system This allows you to quickly assemble vectors and test different ideas. We are a huge...

20. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ...identified. While LY6A or other Ly6 gene family members are linked to viral susceptibility (mouse adenovirus...