We narrowed to 708 results for: ESC

...instead to use a vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters...

...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...

...Gateway technology relies on the two reactions described below: The BP Reaction takes place between the...

... on a new feature. We start with a story that describes the feature and what the criteria is for accepting...

...excellent protocol paper by the Feng Zhang lab describing construction, lentiviral production, transduction...

...of data. In this two-part blog series, we will describe the importance of bioinformatics at Addgene and...

...two pieces: a single protein, computationally described as a fusion of four Cas7 proteins and a putative...

...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  Resources...

...as potent and specific? The two AAV strategies described above showed successful target modification, indicating...

...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...

... Signal Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/...

...free of contamination and match their original description. The oldest, largest, and most diverse of these...

...of mouse transgenics. Everything that has been described is intended for the mouse; however, all the steps...

...don't see protein overexpression? The strains described above should generate sufficient expression levels...

...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...

...performed best when paired with the V106W mutation (described earlier) to minimize off-target editing. Base ...

...the retroviral life cycle for a more detailed description). Since the integrated portion does not contain...

...Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In situ hybridization...

... the first demonstrated use of prime editing to rescue a genetic disease phenotype in an animal model....

... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...