...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...

...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

... the first demonstrated use of prime editing to rescue a genetic disease phenotype in an animal model....

...the retroviral life cycle for a more detailed description). Since the integrated portion does not contain...

...bioinformatics/btz867 Haeussler M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S...

...Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In situ hybridization...

... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...

...Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31...

...optimized, it’s important to ascertain how long the rescue phenotype will last, and more importantly, if it...

...ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying...

... a specific edit, such as the insertion of a fluorescent tag or the introduction of a specific mutation...

...histone modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers...

... capsid of another. Formally, pseudotypes are described with two numbers separated by a slash: the first...

...genome.   References and Resources References Feschotte C, Pritham EJ (2007) DNA Transposons and the Evolution...

...3rd year graduate student in the lab of Prof. Francesco Paesani at University of California San Diego....

...PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted optical control of neural...

...are designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila...

...Fact! Even though the Cas9:RNA complex is well described in the closed conformation, recent electron microscopy...

...’s first demonstration of prime editing used to rescue a genetic disease phenotype in an animal model,...

... with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use...Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru...An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru...