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...you want to fuse your protein to any tags or fluorescent markers? If you’re creating a multigene construct...

...instead to use a vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters...

...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...

...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...

...vectors are the solution. Flex vectors expressing fluorescent cytoplasmic markers (e.g. eYFP) and synaptic ...

... Signal Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/...

...free of contamination and match their original description. The oldest, largest, and most diverse of these...

...of mouse transgenics. Everything that has been described is intended for the mouse; however, all the steps...

...don't see protein overexpression? The strains described above should generate sufficient expression levels...

...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...

...bioinformatics/btz867 Haeussler M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S...

...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...

...performed best when paired with the V106W mutation (described earlier) to minimize off-target editing. Base ...

...the retroviral life cycle for a more detailed description). Since the integrated portion does not contain...

... the first demonstrated use of prime editing to rescue a genetic disease phenotype in an animal model....

...Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31...

...Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In situ hybridization...

...optimized, it’s important to ascertain how long the rescue phenotype will last, and more importantly, if it...

...ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying...