We narrowed to 711 results for: ESC

1. An Inside Look into Addgene's Dev Team

   Type

   Blog Post

   ... on a new feature. We start with a story that describes the feature and what the criteria is for accepting...

2. Career Insights: Technical Support Specialist

   Type

   Blog Post

   ..., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also...

3. Changing Labor Laws Bring Increased Postdoc Wages

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   Blog Post

   ...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...

4. CRISPR Activation: A Practical Guide

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   Blog Post

   ... Signal Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/...

5. The Challenges of Cell Culture

   Type

   Blog Post

   ...free of contamination and match their original description. The oldest, largest, and most diverse of these...

6. Important Considerations in Optogenetics Behavioral Experiments

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   Blog Post

   ...of mouse transgenics. Everything that has been described is intended for the mouse; however, all the steps...

7. Plasmids 101: E. coli Strains for Protein Expression

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   Blog Post

   ...don't see protein overexpression? The strains described above should generate sufficient expression levels...

8. Data Freedom: The Expansion of Data Sharing in Research Publications

   Type

   Blog Post

   ...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...

9. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   Blog Post

   ... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...

10. Hot Plasmids: Summer 2024

    Type

    Blog Post

    ... the first demonstrated use of prime editing to rescue a genetic disease phenotype in an animal model....

11. CRISPR 101: Validating Your Genome Edit

    Type

    Blog Post

    ...bioinformatics/btz867 Haeussler M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S...

12. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    ...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

13. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type

    Blog Post

    ...the retroviral life cycle for a more detailed description). Since the integrated portion does not contain...

14. Plasmids 101: Biotinylation

    Type

    Blog Post

    ...Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In situ hybridization...

15. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    ...Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31...

16. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post

    ...optimized, it’s important to ascertain how long the rescue phenotype will last, and more importantly, if it...

17. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    ...ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying...

18. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    ... a specific edit, such as the insertion of a fluorescent tag or the introduction of a specific mutation...

19. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    ...histone modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers...

20. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    ... capsid of another. Formally, pseudotypes are described with two numbers separated by a slash: the first...