Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 807 results for: Eng;

Showing: 561 - 580 of 807 results

  1. Tips for arabidopsis transformation

    Type
    Blog Post
    ...). Additional resources on the Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24...pSiM24 for simplifying plant genetic engineering Using arabidopsis in the classroom Resources on Addgene.org...

  4. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...Cpf1-mediated cleavage are 42-44 nucleotides in length, about the same size as Cas9’s, but with the direct...Figure from Kleinstiver et al., 2016. Cpf1’s strength may lie in its low off-target editing rates, determined...

  5. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ... less time consuming and/or less technically challenging. Alongside there is a second-line team that takes...are just so many things which can go wrong in a lengthy protocol. And customers may tell all sorts of details...

  6. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...from flow cytometry experiments can be a little challenging to understand if you’ve never done any flow. ... showing frequency of events on the Y axis and strength of signal read on the X axis may be presented....

  8. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...distinguish between Zika and a closely-related virus, Dengue from multiple sample sources. SHERLOCK could also...://doi.org/10.1126/science.aaf5573 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao...

  10. Bioinformatics at Addgene

    Type
    Blog Post
    ...on-site, but now we were presented with a new challenge: automating the bioinformatics steps needed to... reviewing and testing several platforms for engineering pipelines, we selected Nextflow, a workflow management...

  11. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    .... During a transient leaf transformation, the engineered Agrobacterium strain is brought into contact ..., 2006. 225-229. PubMed PMID: 16739580. Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based...

  12. Lab to Office Culture Shock

    Type
    Blog Post
    ...counter-intuitive, but I find office work more mentally challenging than lab work at times.” Sometimes a desk job...to non-scientists can be a fun and gratifying challenge. You’re also getting the opportunity to learn ...

  13. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...response to requirements that emerged from intensive engagement with a wide range of people at the University... The two guiding principles emerging from the engagement with the Wisconsin research community were to...

  14. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...Central PMCID: PMC384129. Aizenman, Einat, Hanna Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal...PMID: 12718874. Yang, Junjie, Weihong Jiang, and Sheng Yang. "mazF as a counter-selectable marker for unmarked...

  15. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...to the right - the Reddit community is active, engaged, and has great questions! 2. The r/Science subreddit...Getting on a good science career path can be challenging these days, but for many of us, scientist is ...

  16. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...multiple genes by CRISPR/Cas-mediated genome engineering." Cell 153.4 (2013): 910-918. PubMed PMID: 23643243...Addgene Blog Get the Basics of Mouse Genome Engineering with CRISPR Validate Your Genome Edit Learn about...

  18. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...recombinant proteins are typically expressed in E. coli engineered to accomodate high protein loads using inducible...

  19. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...linking the quantity of absorbed light, at a given wavelength, to the concentration of fluorophore in solution...method, where the donor is excited by a specific wavelength of light and the signal is collected by using...

  20. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...called Drosha to pre-miRNA. miRNA plasmids are engineered to skip this first pri-miRNA step. These RNA ...1472-6750-6-1 O’Brien, J., Hayder, H., Zayed, Y., & Peng, C. (2018). Overview of MicroRNA biogenesis, mechanisms...
Showing: 561 - 580 of 807 results