We narrowed to 638 results for: Erf

1. Plasmids 101: Codon usage bias

   Type

   Blog Post

   ... in your expression host of choice, you should perform a functional assay to make sure your protein isn...

2. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   ...identified (Hart et al, Bassik et al). The library performed well in both screens, with the growth screen having...

3. Transferable Skills Guide: External Collaborations

   Type

   Blog Post

   ...you realize. Being at a university provides the perfect platform for practicing initiative and for training...

4. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   ...difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro. Find...

5. 5 Reasons to Use Reddit for Science Communication

   Type

   Blog Post

   ...publically about your work (you can find my tips for a perfect science talk here). Finally, my main content organizer...

6. Tips for CRISPR Gene Editing in Mice

   Type

   Blog Post

   ...generate your pCAG-EGXXFP plasmid? They are the perfect primer sets to use to genotype your pups. A simple...

7. The Challenges of Cell Culture

   Type

   Blog Post

   ...making it easier for comparable studies to be performed on a cell line even decades after it is initially...

8. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   ...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...

9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

   Type

   Blog Post

   ...from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian...

10. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    ...veggies) Though the aptabeacons can be used as powerful biosensors, the bound protein fluorophores and...

11. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    ... vector.  Once the BP and/or LR reactions are performed, the next step is to transform competent E. coli...

12. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    ...discussed some of the general considerations for performing behavioral tests, but now we’ll focus on how ...

13. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    ...together with information about the genome are powerful resources for investigating any biological process...

14. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    ...Fos, Egr3, and Arc. Thus, CRISPRa represents a powerful method to dissect the individual and shared contributions...

15. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type

    Blog Post

    ... plants are known to many as the only Monarch butterfly food. They have chemical pathways generating interesting...

16. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    ... exciting. Practice presenting your chalk talk Perfect your story and delivery. Design simple figures ...

17. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    ...G)? You may wonder if a CRISPR-base editor can perform this change. However, there are no naturally known...

18. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    ...novel PAM recognition In 2015, Keith Joung’s lab performed a series of positive selection screens in bacteria...

19. Hot Plasmids - August 2020

    Type

    Blog Post

    ...pluripotent stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene activation (CRISPRa) are ...

20. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    ... CM, Partridge SR, Iredell JR (2017) Plasmid interference for curing antibiotic resistance plasmids in...