We narrowed to 628 results for: Erf

...from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian...

...veggies) Though the aptabeacons can be used as powerful biosensors, the bound protein fluorophores and...

... vector.  Once the BP and/or LR reactions are performed, the next step is to transform competent E. coli...

...strand breaks it introduces makes it difficult to perform two or more integrations on the genome. Most recently...

...novel PAM recognition In 2015, Keith Joung’s lab performed a series of positive selection screens in bacteria...

...pluripotent stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene activation (CRISPRa) are ...

...enough information there for you to be independent performing the technique you trained to do? Walk through...

...dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction with doxycycline prior...

... for diagnosing and resolving organizational performance and often have to be able to quickly synthesize...

...publically about your work (you can find my tips for a perfect science talk here). Finally, my main content organizer...

... CM, Partridge SR, Iredell JR (2017) Plasmid interference for curing antibiotic resistance plasmids in...

...conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research Conference...

...G)? You may wonder if a CRISPR-base editor can perform this change. However, there are no naturally known...

...discovery, plasmid addiction systems have become a powerful tool to address a diverse set of biological inquiries...

...difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro. Find...

...generate your pCAG-EGXXFP plasmid? They are the perfect primer sets to use to genotype your pups. A simple...

...making it easier for comparable studies to be performed on a cell line even decades after it is initially...

...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...

...in the laboratory mouse, most optogenetics is performed in this species. However, transgenic rat technology...

... expression from high-copy number plasmids and powerful promoters will greatly exceed that of any native...