We narrowed to 1,093 results for: IND

...different or novel tools and assumptions to reveal findings that the originator was oblivious to, no matter...of the experiment would require access to each individual video. To PLOS’s credit, the organization subsequently...recognize that storing data in a sharable form is indeed a significant effort. Our standards of data recording...recording are highly variable and usually left to individual researchers. Yet accurate and comprehensible ...appears to leave the scope of data to be shared to individual authors. Minimally, raw but sufficiently annotated...charges of elitism and for scientists to be hiding behind our jargon and esoterica. Public trust in and funding...

...from the termite hindgut. Want to level up your or your class’s streak plate skills? Find the chromoprotein...Resources on Addgene.org Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids at ...the students to separate out colonies from each individual strain using the streak plate technique. Time...

...homology-directed repair (HDR) of a CRISPR/Cas9 induced double-stranded break. This site-directed genome...rendering the resulting construct resistant to Cas9 induced cleavage. Summary of Site Directed Mutagenesis ...is removed by DpnI restriction digestion leaving behind only the mutagenized plasmid. Although it is commonly...reaction. Ideally, your primers should be free of palindromic and repetitive sequences, but if present, a minor...similar approach can be used to identify Cas9/CRISPR-induced genome modifications. Occasionally, multimerization...Addgene.org Browse Our Molecular Biology Protocols Find Empty Vectors for Your Next Cloning Experiment ...

...copies of the transgene that will recombine independently, cells may acquire one of a variety of colors...extremely versatile technology as recombination can be induced in developing fish via Cre protein microinjection...microinjection or crossing of Zebrabow animals to an inducible Cre line. The Schier group developed Zebrabow as ...powerful tool for understanding cell proliferation. Inducible XFP expression in the Ubow mouse Another murine...CFP, and YFP. After crossing onto a tamoxifen-inducible ubiquitin background, each cell in the mouse that...Köhler, Corinna, et al. "Mouse cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation...

...complexity inside the cell or on its surface. Bear in mind, the voltage setting of the laser impacts the magnitude...While flow cytometry can be used for virtually all kinds of experiments and research fields, for any experiment...After that, the next gates fully depend on your individual experiment. Practical example: lymphocytes in...cellular debris and other larger cells. Note, we don’t mind the warning message at the top, as our cells of ...strategy, I can, of course, also draw multiple individual gates around the populations of interest (Figure...of a quadrant gate, I am free to use multiple individual gates. Note that in this case, square gates were...

...single-component systems take advantage of BcLOV4’s light-induced binding of the plasma membrane. In previous publications...light BcLOV4 undergoes a conformational change and binds the plasma membrane under blue light CRY2-CIB1 heterodimerizes...limitations (Figure 1). Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic...substantial PA accumulates in the plasma membranes, indicated by the PA probe colocalizing with SuperPLDx30....CRY2-CIB, and other optogenetic modules? Or want to find more optogenetic tools in our collection? Check ...

...these types of conflicts is important for the individuals involved, and also for the labs, organizations...they were taking care of that”, because that can indicate confusion around... Ownership: Misunderstandings...tools that can come in handy: Empathy: If you often find yourself in confrontational situations with others...the age of five. But it does mean that even if you find someone annoying or disagreeable, you recognize ...Definitely not. But it can help, and if and when you find yourself transferring this skill to another job,...for avoiding, competing, or accommodating, if you find yourself gravitating most frequently towards these...

..., 2008). Shortly after though, three groups independently tried reproducing these results and determined... and part B. Each piece of the gene is then independently packaged into an AAV. When a cell is co-infected...Viral Vector Packaging Capacity in a Transgene-independent Manner. Molecular Therapy 16:124–130 . https:...AAV Transductifon Is Mediated via a DNA-PKcs-independent, Rad51C-dependent Repair Pathway. Molecular Therapy...ddv386 Additional resources on the Addgene blog: Find tips for a first time AAV user Learn about how AAV...on Addgene.org: Check out the Addgene AAV guide Find ready-to-use AAV preps from Addgene's viral service...

...on Addgene.org Find Synthetic Biology Plasmids Find Bacterial Expression Systems Find Plasmids for Protein...optimized for parameters such as induction temperature and length of induction, which will vary between different...gels containing Phos-Tag, a small molecule that binds specifically to phosphate moieties and promotes ...

...Advances in Cre-lox recombination have included inducible Cre, such as the tamoxifen-responsive CreERT, ...single-cell labeling is necessary to distinguish individual cells. The Brainbow-1.0 construct contains three...mice.) The combinatorial effect of multiple, independent recombination events leads to a rainbow of colors... with the vast array of colors needed to mark individual neurons, the system also suffered from a number...they screened a variety of fluorescent proteins to find those with ideal characteristics (low aggregation...Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0, 1.1, ...

...science funding, limitations to facilities and indirect costs, and increased tariffs present an unprecedented... of the cost of creating and verifying them independently — particularly valuable when budgets are constrained...preparations, and antibody production that labs may find increasingly difficult to maintain or access. These...applications, addressing the new funding structures and indirect cost caps more efficiently. Offering free educational... seeking and expanding our collaborations with industry and public and private partners to create additional...

...similarly to their untagged counterparts. These findings motivated Georgieva et al. to develop new tags...from non-vertebrate systems. Preliminary testing indicated that inntags IT5, IT6, and IT10 expressed well...on these parameters. IT5 and IT6 displayed good binding efficiency in both immunofluorescence and immunoprecipitation...for both applications. To prove that inntags are indeed innocuous, Georgieva et al. created tagged versions...detrimental effects on cell size and growth rate, indicating that they compromised protein-protein interactions...

...the toolbox, with the first paper identifying a binding receptor published last year (Dhungel et al., 2025...for your experiment? Use Addgene’s AAV Data Hub to find experimental reports on commonly used vector cargo...Serotype 4 (AAV4) and AAV5 Both Require Sialic Acid Binding for Hemagglutination and Efficient Transduction...Viswanathan, S., Gaj, T., Lavzin, M., Ritola, K. D., Lindo, S., Michael, S., Kuleshova, E., Ojala, D., Huang..., M. J., Chiorini, J. A., & Zabner, J. (2001). Binding of adeno-associated virus type 5 to 2,3-linked ...α2,6 N-Linked Sialic Acids Facilitate Efficient Binding and Transduction by Adeno-Associated Virus Types...

...protection against infectious agents. Several enzymes behind this immunity have already been discovered and ...coli bacteria strain. They found that Cas14 could bind and cleave a targeted sequence of ssDNA. Unlike ...cleavage, the authors showed that Cas14 could indiscriminately cleave ssDNA in trans like Cas13 and Cas12...of dsDNA and RNA in trans. Fluorescent signal indicates the presence of this viral sequence in the assay...our CRISPR cheat sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR guide...

...Advisor/PI who will also be a good mentor? Where can I find a Mentor? What is this I hear about “group” or “...“A good mentor has your goals/needs/situation in mind, rather than (or as well as) their own.” “Someone...mentors/advisors like Yoda? If so, you might want to find additional sources of wisdom. While somewhat lighthearted...on the blog “Tenure, She Wrote” is titled, “What Kind of Mentor Do I Want To Be?” In this excellent essay...the northeast, writes a thoughtful treatise on the kind of advisor/mentor she wants to be for her new lab...

...using AAVs Resources on Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors... different parts of the brain is mediated via individual neurons. Neurons are composed of a cell body,...axons directly to the prefrontal cortex, but also indirectly via synapses to neurons in other brain regions...and aid in visualization of the morphology of individual neurons, including dendritic and axonal extensions...these neurons fluoresce in different colors. An individual neuron can then be traced throughout the brain...Viswanathan, S., Gaj, T., Lavzin, M., Ritola, K.D., Lindo, S., Michael, S., Kuleshova, E., Ojala, D., Huang...

...spinal muscular atrophy, the molecular mechanisms behind the disease are unclear. Dr. Di Schiavi plans on...concrete understanding of the molecular biology behind SMN1 and its interaction partners, Dr. Di Schiavi...suffering from this little-known disease. You can find more information about the disease on the Meothelioma...suffering from rare diseases. These underrepresented individuals deserve a voice. Check back with Addgene often...Countries with Seeding Labs Resources on Addgene.org Find Plasmids for Disease Research ...

...have rapidly been developed. We asked the individuals behind some of these new platforms what their motivations...collaborations within the scientific community The team behind Crowdfight COVID-19 strives to match up scientists...ultimately treatments in a timely fashion.”  With this in mind, Bourque and Tara Moriarty, an Associate Professor...extremely important part of the future of research. To find other COVID-19 databases and resources, check out...

...: 4045853.  Find other plant resources at Addgene Check out Addgene’s plant vectors! Find the plasmids...Agrobacterium tumifaciens. Agrobacterium carries a “tumor-inducing” or Ti plasmid, which allows it to transfer genetic...during preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary Ti ...References Sahoo, Dipak Kumar, Nrisingha Dey, and Indu Bhushan Maiti. "pSiM24 is a novel versatile gene...

...different constructs and cell types. Second, Cre may induce recombination at pseudo- or cryptic loxP sites ...proliferating cells. A Cre-estrogen receptor ligand binding domain-fusion can prevent this toxicity, but with...offspring carried both the recombinase and GAL4, indicating that the recombinases were inherited normally...10.1073/pnas.1111704108. Epub 2011 Aug 9. PubMed. Find the Plasmids @Addgene: Plasmids for KD, R, B2, ...-665. PubMed. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. Loonstra...