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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 935 results for: Ina

Showing: 561 - 580 of 935 results

  1. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... approach, and conclusions (see above). Keep original files in a separate folder: You will often use...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...that was temporarily used. Just be sure that the original code remains in tact! Take notes Comment in ...

  2. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...support the community in developing better, more sustainable, easily sharable products. Open-Source Microscopy...makerspace networks and training users via workshops, seminars, or hackathons greatly increases accessibility...brings stronger networking across laboratory and disciplinary boundaries and can allow for innovative organizations...

  3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...insert targeted mutations through homologous recombination. To further increase the utility of CRISPR/Cas9...containing the RNA pol III promoter, gRNA and pol III terminator. These relatively large cassettes (considering...Size is also a factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA...

  4. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...questions the conclusion of inter-homologue recombination was released. This blog has not been updated...al. were interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic ...embryos to find those that do not carry a given dominant disease-causing gene, or that carry only one copy...

  6. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...yellow, or magenta, depending on the hairpin combination bringing the total number of individual colors...Adding an additional hairpin/fluorescent protein combination, for example, with a far-red FP, would increase...15! They also envision using CRISPRainbow in combination with gene editing. CRISPRainbow requires very...

  7. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...available (below). However, on November 22nd a preliminary injunction against the FLSA updates was granted...inconsistencies across geographical regions When we originally started gathering this information, many assumed...to pursue academic work, and to enhance the sustainability of the scientific enterprise by reducing the...

  8. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...ligation assay (PLA). The clever combination of FUNCAT and PLA PLA was originally published by the Söderberg...16590564. PubMed Central PMCID: PMC222790. 6. Buxbaum, Adina R., Bin Wu, and Robert H. Singer. "Single β-actin...

  9. Summer SciComm: Preprints

    Type
    Blog Post
    ...or awaiting a final publication in a journal.  Preprints can be one tool in a combination of strategies... Addgene ID for their deposited plasmids in the final journal publication or an updated preprint, further...

  11. The time and cost required to make a plasmid

    Type
    Blog Post
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...cloning, waiting, and repeating before you can finally dive deep into generating the data you’re excited...cost $155.25 in reagents and consumables today. Finally, this doesn’t account for the most valuable component...

  12. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... deletions.   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s primary difference...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...

  13. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...drug discovery process.” The ClonTracer library originally came about as Bhang and co-workers were trying...treatment process. The authors went on to show that combination treatment with multiple drugs reveals even smaller...heterogeneity to drug discovery and the development of combination therapies.         References 1. Hyo-eun, C. ...

  14. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ... the protein or structure of interest. For a C-terminal tag, as shown in the example above, we inserted...would be transcribed and translated. For an N-terminally tagged protein, we used the same strategy, inserting...tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional...

  15. Engaging with science and society at pgEd

    Type
    Blog Post
    ... incident, and the sterilization of women in marginalized communities have created a legacy of distrust...included taking extra coursework and attending seminars in bioethics, public health, science policy, and...advantage of their academic environment and attend seminars, symposia, or courses in social, health or policy-related...

  16. Lab Automation at Addgene

    Type
    Blog Post
    ...is ideal because it eliminates the potential of human error or cross contamination between samples. With... Shannon Rinaldi...

  17. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...dCas9. Within a few rounds of cell division, the original methylated strand is diluted to undetectable amounts...dCas9 so that it may later be excised by Cre recombinase. Addgene has several great options: pLV hUbC-...L, Li C, et al. (2018) OGT binds a conserved C-terminal domain of TET1 to regulate TET1 activity and function...

  18. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...used for this study at Addgene which include a C-terminal deletion in the spike protein to improve expression...pseudoviruses to determine how effective sera from vaccinated individuals are at neutralizing the different...

  19. Tips for Technical Support Calls

    Type
    Blog Post
    ...majority of our plasmids and pooled libraries were originally described in a peer-reviewed scientific publication... our thousands of depositors. That means their original articles are your best bet to figure out how to...asking to avoid unrealistic expectations. And finally, provide feedback. If you are unsatisfied with ...

  20. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...PCR product that comes from amplification of the original plasmid which can over-inflate results. A packing...vectors carrying fluorescent markers are used as it eliminates the need for antibody staining. Check Out Our...into a target cell’s genome. This approach can discriminate multiple integration events, reducing the possibility...
Showing: 561 - 580 of 935 results