Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 1,035 results for: LAS

Showing: 561 - 580 of 1035 results

  2. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...targeted strategy, etc.) and episomal expression will last ~2-7 days at moderate levels. With regard to target...

  3. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world...

  5. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ... the direct reprogramming of mouse embryonic fibroblasts (MEFs) into induced endoderm progenitors (iEPs... were partially reprogrammed and re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end... on Addgene.org Visit our stem cell page Find plasmids for reprogramming and differentiation Check out...

  6. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...different cellular environments such as retina or glioblastoma). They also found that exo-AAVs were more resistant...Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving iodixanol gradients...brain tumor stromal cells in a mouse model of glioblastoma (Volak et al. 2018). The structure of exosome...

  9. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465) are now available as 20...Here are some of the AAV we have released in the last few months. You can also browse our entire AAV inventory...

  10. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR..., give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor...

  11. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...Addgene Wesbite Find Reprogramming Plasmids Find Other Hot Plasmid Tools Visit Our Stem Cell Pages ...gratifying to see that Addgene has ensured that plasmid acquisition is not a rate-limiting step in stem...

  12. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...enough. For example, if we need to develop a new plasmid collection page, we certainly don't expect that...design every icon, curate all of the appropriate plasmids, and code the entire page. That would be entirely...Addgene Website Are You a Molecular Biologist? Find Plasmids for Your Research Here!        ...

  13. How Addgene Creates Content

    Type
    Blog Post
    ...that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website,...mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists ...Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

  14. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ... give as a journal club to my molecular biology class. A Cell paper. With six figures. Averaging ten panels... assignment was clear: present a paper to our classmates so they could understand it. When my mind went...incidents above–and the dumpster noises were just last week!  Nerves It’s completely normal to be anxious...

  15. To Each HIS Own

    Type
    Blog Post
    ... tag plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing...from Addgene More Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies...

  16. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...Flash quiz! What pops into your head when you hear the phrase “action potential?” Firing neurons are ...through gap junction proteins which connect the cytoplasm of two cells) can also affect patterning and in...GEVI features increased brightness and improved plasma membrane specificity. Stable Step Function Opsin...

  17. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...to antibodies. Instead, they are derived from a class of binding proteins known as ankyrin repeat proteins...Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs...available from AddgeneBrowse our recombinant antibody plasmid collection References Grumezescu, A. M. (2018)....

  18. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s primary difference from Cas9.... Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell, 163(3): 759-771 (2015...More resources on the Addgene Website CRISPR Plasmids and Resources CRISPR Guide  ...

  19. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...double-membraned structures containing undigested cytoplasmic contents. Autophagosomes that have fused with... example of what fluorescence looks like for cytoplasmic mitochondrial Rosella with and without autophagy... Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent Protein Guide: Biosensors...
Showing: 561 - 580 of 1035 results