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We narrowed to 1,039 results for: LAS

Showing: 561 - 580 of 1039 results
  1. How Exercise Purges Stress and Keeps Depression at Bay

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    Blog Post
    ...circumstances – including unpredictable loud noises and flashing lights - that sent average, control mice into ...come to the right place. Get started with PGC-1 plasmids from Addgene. References: Skeletal muscle PGC....2014.09.012. PMID: 25295784 Related Materials: PGC-1a Plasmids      ...
  2. Why Do I Need an MTA Anyway?

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    Blog Post
    ...for material transfers. When Addgene entered the plasmid sharing space, we heard over and over again that...with the UBMTA result in quick approvals, so your plasmid requests can get to your lab faster. For Everybody...reference pages. So go ahead and use Addgene plasmids in your experiments. You know that they’re properly...
  3. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    Blog Post
    ...Cas9 cleavage when using a photomasking technique. Last, Hemphill et al. presented data showing that this...S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek...Optogenetics and CRISPR: A New Relationship Built to Last. In: Methods in Molecular Biology. Springer US, ...Website Browse Our Collection of Optogenetics Plasmids Check out Our Genome Engineering Resources Brush...
  4. Deep Dive: qPCR

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    Blog Post
    ... Though you’ll want to predict this value using BLAST, you’ll need to experimentally verify once you’ve...11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl More resources on the Addgene blog...blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...
  5. Six Spooky Science Stories and Halloween at Addgene

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    Blog Post
    ...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding... contest trophy. We’ve had teams dressed up as plasmids, Blugene, and the Addgene ping pong table. This... of the algae cell is just the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not...
  6. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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    Blog Post
    ...Table 1). The four systems Name Function Plasmid Transduction Marker Mouse Strain C-CHIME...  The X-CHIME deposit also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations...first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral Fellow...
  7. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

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    Blog Post
    ...viral vectors (AAV), neuroscientists can combine classical genetic tools with the manipulation of specific...combinations to choose from for your experiments! Late last year, we hosted our first AAV Education and Development...of molecular tools and sensors Combinations of classical genetic tools, for example Cre/loxP or Flp/FRT...
  8. New Norepinephrine Indicators: nLightG and nLightR

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    Blog Post
    ...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly...
  9. Antibodies 101: Fc Effector Functions

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    Blog Post
    ...These receptors are specific to isotype class or even subclass, and are part of the reason why different...antibodies on a pathogen activates the immune system's classical complement pathway, starting a cascade of events...
  10. CASTing Off for New Shores in Human Genome Editing

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    Blog Post
    ...moved a gene required for phage propagation to a plasmid that lacked a promoter — only CAST integration ...integrate DNA payloads of up to 15 kb. Find evoCAST plasmids here! The new horizons of evoCAST In the emerging...Addgene's CRISPR Guide (Jump to Large Edits) CAST Plasmid Collection ...
  11. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

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    Protocol
    ...Modify your plasmid MCS or add short sequences using oligo overlap cloning.... Protocols Plasmid Modification...Modification by Annealed Oligo Cloning Plasmid Modification by Annealed Oligo Cloning You may also like... Restriction...Restriction Digest of Plasmid DNA DNA Ligation Bacterial Transformation Summary Oligo overlap cloning can...anytime you need to add a short stretch of DNA to a plasmid, such as: Changing a Multiple Cloning Site (MCS...
  12. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

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    Protocol
    ...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Will...sites to your recipient plasmid : You can modify the MCS of your recipient plasmid using Annealed-oligo ...material. We recommend 1.5-2μg of donor plasmid and 1μg of recipient plasmid. It is also critical that as much...re-circularization of the recipient plasmid. You should treat your digested recipient plasmid with a phosphatase prior...ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone ... on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence...
  13. Meet the New Addgene Board Members!

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    Blog Post
    ...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world...
  14. Single-cell tracking of lineage and identity with CellTag

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    Blog Post
    ... the direct reprogramming of mouse embryonic fibroblasts (MEFs) into induced endoderm progenitors (iEPs... were partially reprogrammed and re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end... on Addgene.org Visit our stem cell page Find plasmids for reprogramming and differentiation Check out...
  15. Viral Vectors 101: Transductions

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    Blog Post
    ...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...targeted strategy, etc.) and episomal expression will last ~2-7 days at moderate levels. With regard to target...
Showing: 561 - 580 of 1039 results