We narrowed to 1,039 results for: LAS

...circumstances – including unpredictable loud noises and flashing lights - that sent average, control mice into ...come to the right place. Get started with PGC-1 plasmids from Addgene. References: Skeletal muscle PGC....2014.09.012. PMID: 25295784 Related Materials: PGC-1a Plasmids      ...

... Sequencing Resources: Tips for Using BLAST to Verify Plasmids Acknowledgements: Thank you BitesizeBio...on BitesizeBio here. As part of my job ensuring plasmid quality at Addgene, I analyze 50-100 sequencing...

...for material transfers. When Addgene entered the plasmid sharing space, we heard over and over again that...with the UBMTA result in quick approvals, so your plasmid requests can get to your lab faster. For Everybody...reference pages. So go ahead and use Addgene plasmids in your experiments. You know that they’re properly...

...Cas9 cleavage when using a photomasking technique. Last, Hemphill et al. presented data showing that this...S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek...Optogenetics and CRISPR: A New Relationship Built to Last. In: Methods in Molecular Biology. Springer US, ...Website Browse Our Collection of Optogenetics Plasmids Check out Our Genome Engineering Resources Brush...

... Though you’ll want to predict this value using BLAST, you’ll need to experimentally verify once you’ve...11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl More resources on the Addgene blog...blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding... contest trophy. We’ve had teams dressed up as plasmids, Blugene, and the Addgene ping pong table. This... of the algae cell is just the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not...

...Table 1). The four systems Name Function Plasmid Transduction Marker Mouse Strain C-CHIME...  The X-CHIME deposit also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations...first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral Fellow...

...viral vectors (AAV), neuroscientists can combine classical genetic tools with the manipulation of specific...combinations to choose from for your experiments! Late last year, we hosted our first AAV Education and Development...of molecular tools and sensors Combinations of classical genetic tools, for example Cre/loxP or Flp/FRT...

...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly...

...Addgene.org Find plasmids for in vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent...acoustic reporters for your research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau...

...These receptors are specific to isotype class or even subclass, and are part of the reason why different...antibodies on a pathogen activates the immune system's classical complement pathway, starting a cascade of events...

...moved a gene required for phage propagation to a plasmid that lacked a promoter — only CAST integration ...integrate DNA payloads of up to 15 kb. Find evoCAST plasmids here! The new horizons of evoCAST In the emerging...Addgene's CRISPR Guide (Jump to Large Edits) CAST Plasmid Collection ...

...Modify your plasmid MCS or add short sequences using oligo overlap cloning.... Protocols Plasmid Modification...Modification by Annealed Oligo Cloning Plasmid Modification by Annealed Oligo Cloning You may also like... Restriction...Restriction Digest of Plasmid DNA DNA Ligation Bacterial Transformation Summary Oligo overlap cloning can...anytime you need to add a short stretch of DNA to a plasmid, such as: Changing a Multiple Cloning Site (MCS...

...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Will...sites to your recipient plasmid : You can modify the MCS of your recipient plasmid using Annealed-oligo ...material. We recommend 1.5-2μg of donor plasmid and 1μg of recipient plasmid. It is also critical that as much...re-circularization of the recipient plasmid. You should treat your digested recipient plasmid with a phosphatase prior...ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone ... on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence...

...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world...

...depositing plasmids pre-publication Find molecular biology protocols Find eBooks on plasmids, CRISPR, viral...community.  Be the peer-reviewer you wish for The last thing that I wanted to mention about the peer-review...

... the direct reprogramming of mouse embryonic fibroblasts (MEFs) into induced endoderm progenitors (iEPs... were partially reprogrammed and re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end... on Addgene.org Visit our stem cell page Find plasmids for reprogramming and differentiation Check out...

...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...targeted strategy, etc.) and episomal expression will last ~2-7 days at moderate levels. With regard to target...

...Addgene.org Browse all fluorescent protein plasmids Find plasmids for FRET Check out the Michael Davidson...Looking for a bright, monomeric FP published in the last few years with an emission peak around 600nm?  Simply...

... to our volunteer list. We then collected and classified their responses, and used that information to... favors from becoming starting points for long-lasting partnerships Join us! We are a community of researchers...Resources on Addgene.org Browse Addgene's COVID-19 plasmids and resources ...