We narrowed to 817 results for: MAL
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TypeBlog Post...fluorescent proteins that behave as monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen,...therefore consisted of glycines, serines and other small, non-aliphatic amino acids. However, long linkers...
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Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post... a homogenous cell population in culture, it is normal to observe diversity in cell size, marker expression...s origin mainly stem from cell debris and other small fragments. Where exactly a given cell population... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post... conformational complexity There are 24 known mammalian integrins, all heterodimers composed of one of...sequences that imitate this motif and compete with both small molecule integrin inhibitors and natural ligands... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...populations. To decrease the animal breeding necessary to produce animals with Brainbow transgenes and... -
A Control for All Seasons
TypeBlog Post...antibody is replaced with pre-immune serum from the animal the antibody was produced in or an isotype-matched...primary antibody is incubated with its antigen or a small epitope-containing peptide to block the antigen-... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...of endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an ...experiments. Wavelengths Most vectors have an optimal wavelength to use but can be activated by a range... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...), but there are differences in tropism between animal species or even strains of the same species. What... to define high titer, then, is this: It’s the optimal number of viral particles per volume required to... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post... frozen or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...in the uncut DNA; the rest of the digest appears normal and produces the expected fragments for that plasmid...transfected supercoiled plasmid DNA concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi:... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...its target states are only represented in a very small number of registers. Instead, one may use qPCR to... not be suitable for applications that require minimal cellular perturbation. Also, this method requires... -
Finding Your Perfect Job After University
TypeBlog Post... agencies and noticed a position available at a small science non-profit doing customer service (recruitment...this is a fairly unique situation, because it is a small and dynamic company and so it has the flexibility... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...collaborative learning style at all. Finally I rotated in a small lab with a new PI. I realized that as a first born...collaboration? If you are considering a career at a small, teaching college you might want to consider less... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...experiments. Biology is messy enough that by chance, some small number of the scientists will get a result that ...order to chase a positive result. Beyond these more formal systems, individual labs can certainly institute... -
Quick Guide to All Things Lentivirus
TypeBlog Post...development of viral vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic...If not using proper technique, there’s always a small chance that a researcher could infect him or herself... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...Moreau (1896) where he explores the concepts of animal testing and modifications. The premise of the novel... novel involves Dr. Moreau who creates human-animal hybrids. This book is published 30 years after the... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post... (22). Large purine:purine mismatches and most smaller pyrimidine:pyrimidine mismatches are typically ...screened per plate. The results indicated that the optimal buffer for Taq DNA Ligase contains 100 – 200 mM... -
Transferable Skills Guide: Teamwork
TypeBlog Post...found that many researchers feel that they lack formal training in a variety of transferable skills. At...until the team really starts functioning (or malfunctioning). Clear lines of communication Few people actually... -
Finding Your Science Policy Path
TypeBlog Post...societies, and industry through convening activities, formal lobbying, or strategy setting. The “little p” ...question may change over time and that’s perfectly normal! Action Items: Start a career journal during... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...ninety-nine cubits of Aristotle, nor would you mention my small error and at the same time pass over in silence ...she studies the development and regeneration of mammalian taste buds. Erin is also the President of Project... -
Antibodies 101: Flow Cytometry
TypeBlog Post...antibodies to pass through the cell membrane. Formaldehyde fixation creates cross-links between lysine ...Sometimes, users will combine methods and fix with formaldehyde first to freeze everything in place followed...