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Showing: 561 - 580 of 811 results

  1. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...of endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an ...experiments.  Wavelengths Most vectors have an optimal wavelength to use but can be activated by a range...

  2. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...), but there are differences in tropism between animal species or even strains of the same species. What... to define high titer, then, is this: It’s the optimal number of viral particles per volume required to...

  4. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...in the uncut DNA; the rest of the digest appears normal and produces the expected fragments for that plasmid...transfected supercoiled plasmid DNA concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi:...

  6. Finding Your Perfect Job After University

    Type
    Blog Post
    ... agencies and noticed a position available at a small science non-profit doing customer service (recruitment...this is a fairly unique situation, because it is a small and dynamic company and so it has the flexibility...

  8. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...collaborative learning style at all. Finally I rotated in a small lab with a new PI. I realized that as a first born...collaboration? If you are considering a career at a small, teaching college you might want to consider less...

  9. Quick Guide to All Things Lentivirus

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    Blog Post
    ...development of viral vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic...If not using proper technique, there’s always a small chance that a researcher could infect him or herself...

  11. Finding Your Science Policy Path

    Type
    Blog Post
    ...societies, and industry through convening activities, formal lobbying, or strategy setting.  The “little p” ...question may change over time and that’s perfectly normal!  Action Items:  Start a career journal during...

  12. A History of Genome Engineering in Popular Culture

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    Blog Post
    ...Moreau (1896) where he explores the concepts of animal testing and modifications. The premise of the novel... novel involves Dr. Moreau who creates human-animal hybrids. This book is published 30 years after the...

  13. Transferable Skills Guide: Teamwork

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    Blog Post
    ...found that many researchers feel that they lack formal training in a variety of transferable skills. At...until the team really starts functioning (or malfunctioning). Clear lines of communication Few people actually...

  14. Antibodies 101: Flow Cytometry

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    Blog Post
    ...antibodies to pass through the cell membrane. Formaldehyde fixation creates cross-links between lysine ...Sometimes, users will combine methods and fix with formaldehyde first to freeze everything in place followed...

  17. New Antibody Animation!

    Type
    Blog Post
    ..., and the different antibody isotypes found in mammals.      Subscribe to Addgene's YouTube channel ...

  19. The 12 Days of CRISPR

    Type
    Blog Post
    ...Cas13 variants for targeted RNA degradation with minimal collateral effects🎵 On the 1st day of CRISPR, ...
Showing: 561 - 580 of 811 results