Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 909 results for: Nes;

Showing: 561 - 580 of 909 results

  1. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...The degradation tag (dTAG) system functions by harnessing the power of a novel degrader of the 12 kDa protein...round of evolution (AID-2) to reduce some of the leakiness associated with the system, but the general concept...all the tags discussed here and profile their effectiveness with over a dozen different proteins. In the...

  2. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...and departments will have qPCR machines available for use. ddPCR machines are not as commonly available...measurement of the low-affinity Fc gamma receptor genes FCGR3A and FCGR3B. PloS one, 10(1), e0116791. https...

  3. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase chain reaction, qPCR...interest  Molecular Cloning Site-directed mutagenesis Molecular Diagnostics qPCR Quantify starting... PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase...

  4. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...Manuel Leonetti to tag all 22,000 protein coding genes in the human genome with fluorescent proteins. For...tool Crispor by Max Haeussler. With a few extra lines of code, we enabled it to return guides and primers...which is important for quality control. The only ones we found that worked with NGS data were: Crispresso...

  5. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...reaction but can be used to quickly generate multiple clones. Difficulty: If you’re an experienced cloner, you...also quite efficient; although efficiency often declines as the number of fragments you’re hoping to assemble...out protocols for plasmid cloning Find vector backbones Read our molecular biology reference ...

  6. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...are millimeters in thickness (8,9).  In addition, there is a drop in image brightness with increasing depth... Ultramicroscope.  In addition, adjusting the thickness of the laser sheet and using multi-view imaging...

  7. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...should be run in duplicate or triplicate. The closeness of the data points generated by replicate values...be linear or nonlinear. BCA assays have linear trendlines while those for ELISAs and other enzymatic assays...advanced graphing software designed to plot complex trendlines. If you don’t have access to this kind of software...

  8. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... Joseph Provost, then a new faculty member at Minnesota State (and now Professor of Chemistry and Biochemistry...my students published their work identifying new genes required for stem and progenitor cell maintenance...for increasing diversity and inclusion in STEM disciplines (Bangera & Brownell, 2014; Duboue et al., 2022...

  9. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...teammates want to be assured that you will contribute openness, clarity, approachability, and professionalism...you can learn more about possible strengths and weaknesses, and view everything as a learning experience... weaknesses...

  10. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...editing from the Cas13b REPAIR system To test the robustness of REPAIRv1, the lab targeted 34 pathogenic G...cytosine deaminase. Three rounds of rational mutagenesis on the ADAR2DD residues that contact the RNA ...RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A mutation in RESCUE...

  11. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...students learned something. Based on the initial guidelines I received from the teachers, I formulated the...own wonderful tangents. Some groups asked about vaccines, others were curious about the agricultural repercussions...special thanks to Joanne Marks, Jonathan Marks, and Vanessa Bullard of Chenery Middle School for their tremendous...

  12. Tips for arabidopsis transformation

    Type
    Blog Post
    ...studying transcriptional and translational dynamics of genes and proteins in living plants, to complementing ...process right the first time. I’ll lay out some guidelines here. A detailed transformation protocol, as ...potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop....

  13. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...adapted for a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner...method of tagmentation for next-gen sequencing pipelines (e.g. using Tn5), and as knock-in tools for human...plasmid (pEffector) expresses all seven required genes and the CRISPR RNA under the same promoter, and ...

  15. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...hybrid system (also called the interaction trap), genes encoding proteins of interest(POI’s) are fused to...variations, including many that use auxotrophic rescue genes as reporters and some that repress instead of activate... Vectors Read our Blog Post About Tagging Yeast Genes ...

  16. Pouring LB Agar Plates

    Type
    Protocol
    ...Plasmids can carry one or more antibiotic resistance genes, which confer resistance to a specific antibiotic...

  18. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...sharing information through the use of popular Chinese social media platforms.  As you can imagine, shipping...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...

  19. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome engineering...analysis, they identified 53 potential candidate genes that fell into 3 categories based on the architecture... to bind and cleave target RNAs, although its usefulness in bacterial cells will likely be limited by ...

  20. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...options: Early detection, immunotherapy, and angiogenesis inhibitors New research is also going into two...bevacizumab (Avastin). This drug (known as an angiogenesis inhibitor) prevents the growth of new blood ...asbestos. Nonprofits like the Asbestos Disease Awareness Organization (ADAO) are working toward such a ...
Showing: 561 - 580 of 909 results