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We narrowed to 1,006 results for: RING

Showing: 561 - 580 of 1006 results

  1. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ... how he would go home in the afternoon during the child-rearing years, learning “lots of interesting things...enjoys each other’s company outside of the lab by sharing meals, going on trips, and going to New Hampshire...

  2. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...your experiments. Get organized now! Start by gathering that pile of lab notebooks and binder on your ...frequently. Copy these into an electronic file sharing tool, such as Google Drive or Dropbox, and file...to organize your cryo freezer. No more issues deciphering handwriting or trying to find to where the binder...

  3. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...much easier to get around our site.  Community, sharing, and fun Along with improving search and navigation...the values that make Addgene unique: community, sharing, and fun. The Addgene repository is built on the..., fun, and promote our values of community and sharing.  One big feature upgrade? Blugene now has a full...

  4. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...co-op) is what has brought me, a second year bioengineering student, to Addgene. I am currently working...Addgene, I felt comfortable asking questions without fearing they would be disregarded - everyone was very approachable...comfortable.     Lucie Wilson is a rising junior and bioengineering student at Northwestern University. She is ...

  5. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...disease research and provide hope to patients suffering from these devastating afflictions. Elia Di Schiavi...for peritoneal mesothelioma and thereby relieve suffering from this little-known disease. You can find more...Nabavi and will continue to work to help those suffering from rare diseases. These underrepresented individuals...

  6. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...preferences using HEK293 cells. They started by comparing D10A and H840A total editing efficiency in PAM-in...H840A creates 3’ overhangs in a PAM-out design.  Exploring nickases for homology directed repair The potential... Mary Gearing...

  7. Antibodies 101: Affinity Tags

    Type
    Blog Post
    .... If you decide not to cleave the GST tag after/during purification, be aware that the tag dimerizes in...purifications due to the presence of more naturally occurring histidine-rich proteins in mammals. Mammalian ...possible but requires optimization and often more stringent washing. Nonetheless, this tag can be purified...

  8. Hot Plasmids - October 2022

    Type
    Blog Post
    ...for 2-photon imaging of voltages. This includes monitoring of fast bursts, as well as slow up/down voltage...work also proposes promising avenues for future engineering to shift the spectra and improve the performance...not just because of the title!) The Myc-tag is a string of 10 amino acids, EQKLISEEDL, that can be incorporated...

  9. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ..., had to keep him from running too much as any jarring of his body could initiate very painful FOP flare...donation via the link below. Please continue to help bring HOPE to AJ.   PS: The superhero race is the perfect...running on behalf of AJ Gonzales, a young boy suffering from FOP, raising money for NORD for the past ...

  10. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...obstacles that stand in the way of the doers and by ensuring good communication. A manager must both hire and...delegating tasks and in motivating their doers by ensuring they are as engaged as possible. To learn more...of leader, one needs to take the initiative by bringing and effecting solutions to both known and unidentified...

  11. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...associated genes) system. This popular genome engineering tool offers flexibility, multiplexibility, and...system that scientists have harnessed for genome engineering. The diversity of the CRISPR/Cas systems provides...who has the strongest will to survive. Genome engineering and future directions CRISPR/Cas inspired genome...

  12. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...pR6K ~15-20 R6K* C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300...of replication is referred to as "relaxed" or "stringent" depending on whether the ori is positively regulated...

  14. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ...Agreements, your TTO signs each agreement just once, covering nearly all future requests/deposits. This reduces...Institute  California Institute of Technology Cold Spring Harbor Laboratory  Dana-Farber Cancer Institute...Hutchinson Cancer Research Center Memorial Sloan-Kettering Cancer Center  Monash University  Montana State...

  15. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...different CellTag libraries at multiple timepoints during reprogramming. These library-specific identifiers...different CellTag libraries at three time points during the 28 day study: prior to reprogramming (CellTagMEF...CellTagging. By constructing lineage trees and comparing them with the gene expression changes of more ...

  16. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ...reached out to MIT labs in the Synthetic Biology Engineering Research Consortium. That is how Aditya Kunjapur...robust tools that have practical applications in monitoring the accidental or intentional release of engineered...complementary tools in a time where biological engineering and synthetic biology is expanding rapidly.  ...

  17. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...preventing them from moving forward? Answering these questions will bring you and your audience - via the ...sense of curiosity and wonder why you’re doing and sharing science in the first place?   References and Resources...

  19. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...considerable number of researchers, I had high hopes of securing tenure in a British or Irish University where ...how to sell these products to other scientists. Tailoring my CV for the job To become an attractive candidate...removed my techniques and protocols, courses taken during my PhD and Undergraduate sections and brought in...

  20. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...identify potential career pathways, education and mastering new skill sets must occur by doing work outside...can: Introduce new perspectives in science and engineering Looking at your research from a broader viewpoint...Networking As the number of qualified scientists entering the workforce increases, EAs allow graduate students...
Showing: 561 - 580 of 1006 results