We narrowed to 1,006 results for: RING;

...preferences using HEK293 cells. They started by comparing D10A and H840A total editing efficiency in PAM-in...H840A creates 3’ overhangs in a PAM-out design.  Exploring nickases for homology directed repair The potential... Mary Gearing...

...for 2-photon imaging of voltages. This includes monitoring of fast bursts, as well as slow up/down voltage...work also proposes promising avenues for future engineering to shift the spectra and improve the performance...not just because of the title!) The Myc-tag is a string of 10 amino acids, EQKLISEEDL, that can be incorporated...

.... If you decide not to cleave the GST tag after/during purification, be aware that the tag dimerizes in...purifications due to the presence of more naturally occurring histidine-rich proteins in mammals. Mammalian ...possible but requires optimization and often more stringent washing. Nonetheless, this tag can be purified...

..., had to keep him from running too much as any jarring of his body could initiate very painful FOP flare...donation via the link below. Please continue to help bring HOPE to AJ.   PS: The superhero race is the perfect...running on behalf of AJ Gonzales, a young boy suffering from FOP, raising money for NORD for the past ...

...obstacles that stand in the way of the doers and by ensuring good communication. A manager must both hire and...delegating tasks and in motivating their doers by ensuring they are as engaged as possible. To learn more...of leader, one needs to take the initiative by bringing and effecting solutions to both known and unidentified...

...associated genes) system. This popular genome engineering tool offers flexibility, multiplexibility, and...system that scientists have harnessed for genome engineering. The diversity of the CRISPR/Cas systems provides...who has the strongest will to survive. Genome engineering and future directions CRISPR/Cas inspired genome...

...pR6K ~15-20 R6K* C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300...of replication is referred to as "relaxed" or "stringent" depending on whether the ori is positively regulated...

...screens require a low multiplicity of infection (MOI) during cell library construction to ensure that the majority...screening, calcineurin dynamics measurements, or monitoring in vivo. To overcome these technical issues, ... proteins can bind specific DNA sequences which brings the attached fluorescent protein to the DNA. They...

...Agreements, your TTO signs each agreement just once, covering nearly all future requests/deposits. This reduces...Institute  California Institute of Technology Cold Spring Harbor Laboratory  Dana-Farber Cancer Institute...Hutchinson Cancer Research Center Memorial Sloan-Kettering Cancer Center  Monash University  Montana State...

...different CellTag libraries at multiple timepoints during reprogramming. These library-specific identifiers...different CellTag libraries at three time points during the 28 day study: prior to reprogramming (CellTagMEF...CellTagging. By constructing lineage trees and comparing them with the gene expression changes of more ...

...preventing them from moving forward? Answering these questions will bring you and your audience - via the ...sense of curiosity and wonder why you’re doing and sharing science in the first place?   References and Resources...

...surface display, proteins of interest are fused to anchoring proteins to be expressed on the surface of a host...interested in is available as a viral prep, look under “Ordering” to see what options are offered. Figure...resources on the Addgene blog Viral Vectors 101: Preparing Pooled Libraries Pooled Library Amplifications...

...reached out to MIT labs in the Synthetic Biology Engineering Research Consortium. That is how Aditya Kunjapur...robust tools that have practical applications in monitoring the accidental or intentional release of engineered...complementary tools in a time where biological engineering and synthetic biology is expanding rapidly.  ...

...syndrome, a rare disease that can cause hearing loss and changes in coloring of the hair, skin, and eyes. The...competition for researchers working on rare diseases, offering them the chance to win some of the latest tools...

...considerable number of researchers, I had high hopes of securing tenure in a British or Irish University where ...how to sell these products to other scientists. Tailoring my CV for the job To become an attractive candidate...removed my techniques and protocols, courses taken during my PhD and Undergraduate sections and brought in...

...identify potential career pathways, education and mastering new skill sets must occur by doing work outside...can: Introduce new perspectives in science and engineering Looking at your research from a broader viewpoint...Networking As the number of qualified scientists entering the workforce increases, EAs allow graduate students...

...common ways to quantify DNA. This method involves measuring the absorbance/transmission of light through a...fluorometer to quantify DNA this way. First, start by pouring your gel containing a DNA intercalating dye (ex... the run time is just a few minutes per sample. During the run, DNA fragments move through a micro- or...

...proteins or cells while having little effect on neighboring cells. The photosensitizer used in this work ...effective killing of dL5** cells without death of neighboring bystander wild-type cells.   Zebrafish experiments...photosensitizers had similar rates of off-target damage to neighboring cells. TL;DR MG-2I-dL5** is a NIR photosensitizer...

...hypothesized that she could make enzymes controllable by tethering them to Dronpa, a fluorescent protein known to... HMOs are thought to play an important role in nuturing the development of beneficial bacteria in the ...HMO biosensors and displayed her findings at the Spring 2017 ACS meeting. She hopes these biosensors and...

.... While clearly an outlier, this story had us wondering: is there a larger trend here? Do papers associated...Management. She is particularly interested in exploring how innovative tools influence the direction of...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...