We narrowed to 613 results for: SET

1. Viral Vectors 101: Viral Vector Elements

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   Blog Post

   ...vectors Of the many viruses out there, only a small subset are commonly used as tools in the lab. We will ...

2. Viral Vectors 101: Viral Applications

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   Blog Post

   ...be used to express proteins. Viral expression cassettes typically contain a promoter (inducible or constitutive...

3. Sharing is Caring: But How to Distribute Open Hardware?

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   Blog Post

   ... tailored image processing and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian...

4. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   Blog Post

   ...random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9, which they showed also...

5. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ... vectors for use with their favorite selection cassette. They could also switch from FLAG to GFP or a ...

6. Plasmids 101: Yeast Vectors

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   Blog Post

   ...markers. The MX series of antibiotic resistance cassettes is most commonly employed, with the KanMX being...

7. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   Blog Post

   ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

8. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

9. The Future of Research Symposium Boston 2015

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   Blog Post

   ...University School of Medicine and the University of Massachusetts Medical School. Graduate education programs ...

10. Plasmids 101: Cre-lox

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    Blog Post

    ...either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene...

11. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    Blog Post

    ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

12. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

    ...complex. To help troubleshoot your experimental setup as a potential source of error in suboptimal or ...

13. Viral Vectors 101: Calcium Sensors

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    Blog Post

    ...and is a great FRET sensor option if you have the setup necessary for readout.   We’ve got a live...

14. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    Blog Post

    ...the home cage and places it into the experimental setup, the mouse becomes stressed. It will likely move...

15. Plan Your Experiment

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    Guide

    ...to NHEJ knockout. Base editors can make a limited set of mutations. Repress or Interfere (CRISPRi) Reduce...

16. Plasmids 101: Protein Expression

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    Blog Post

    ...amounts of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein...

17. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post

    ...and a plasmid that contains the Mobile CRISPRi cassette flanked by left and right Tn7 end sequences. Donor...

18. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    Blog Post

    ...after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School....

19. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ...enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle. The earliest explorations...

20. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    Blog Post

    ...As we pointed out last year2, delivering a DNA cassette encoding the cas9 gene and single guide RNAs (...