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...Partitions that did not contain any target DNA at the onset of PCR are negative, whereas those that did are ...

...random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9, which they showed also...

...Recombinant AAV vector with ITRs that flank the gene cassette, which contains a promoter, the gene of interest...

...vectors Of the many viruses out there, only a small subset are commonly used as tools in the lab. We will ...

...be used to express proteins. Viral expression cassettes typically contain a promoter (inducible or constitutive...

... tailored image processing and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian...

...High School Apprenticeship, preparing local (Massachusetts), economically disadvantaged students for paid...

... vectors for use with their favorite selection cassette. They could also switch from FLAG to GFP or a ...

...markers. The MX series of antibiotic resistance cassettes is most commonly employed, with the KanMX being...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

...University School of Medicine and the University of Massachusetts Medical School. Graduate education programs ...

...either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

...complex. To help troubleshoot your experimental setup as a potential source of error in suboptimal or ...

...the home cage and places it into the experimental setup, the mouse becomes stressed. It will likely move...

...and is a great FRET sensor option if you have the setup necessary for readout.   We’ve got a live...

...amounts of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein...

...enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle. The earliest explorations...

...and a plasmid that contains the Mobile CRISPRi cassette flanked by left and right Tn7 end sequences. Donor...