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TypeBlog Post...read the following Top 10 posts of 2014, take some time and see what you’re missing. Let us know if you ... Origin of Replication By Kendall Morgan Save Time and Money by Making Your Own Competent Cells By...
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Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog Post...modify the expression of multiple genes at the same time.” For more details on how to apply the new system...for CRISPR Genome Editing CRISPR-Cas9: Tips for Optimizing sgRNA Activity Lentiviral CRISPR Libraries Enable... -
Addgene Stands with the Scientific Community
TypeBlog Post...collaborative approach eliminates redundant work, saving time and allowing precious research dollars to go further... would be most valuable from Addgene during this time. Your input will directly shape our response initiatives... -
How Do I Place an Addgene Order Using a PO Number?
TypeBlog Post...MTA), which streamlines the process and saves you time. Anyone affiliated with the recipient organization...number that you entered during checkout. At this time, your organization will have net 30 days to complete... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog Post...native protein. Since this process takes a lot of time and energy, Martí Aldea and collaborators have created... will even replace our older epitope tags - only time will tell! References 1. Georgieva, Maya V., et... -
Keeping Up With CRISPR/Cas9
TypeBlog Post...gotten, consider these headlines in The New York Times and Forbes respectively: "A Powerful New Way to ...Emory University summed it up well in that New York Times feature when he said, “All of this has basically... -
What's New in CRISPR - Spring 2019
TypeBlog Post...Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal DNA cleavage at 48 °C, a temperature incompatible...Now, they’ve developed another AAV toolkit - this time using the Staphylococcus aureus Cas9 (SaCas9). This... -
Science Communication Snapshot: DayCon 2015
TypeBlog Post...in the News (SITN), we recently sponsored a first-time event called DayCon. DayCon is a one-day conference...variety of short- and long-form written content about timely scientific topics, as well as archived seminar ... -
Let There Be LITE Plasmids
TypeBlog Post...transcription in as little as 30 minutes after light stimulation. Under those optogenetic controls, gene expression...of neurons, she offers a tip: save yourself some time by making an AAV1 supernatant. (The details can ... -
What's New in CRISPR - December 2019
TypeBlog Post...find more new CRISPR tools for your research! This time: CRISPRi in Burkholderia Base editors CRISPR toolkit...rhamnose-inducible promoter. While this system was optimized for Burkholderia, they identified 67 genera of... -
Stepping into a Virtual Internship: My Experience at Addgene
TypeBlog Post...gain access to an internship on the journey to full-time employment. I have always been interested in the...relating to their roles at Addgene. I had a great time speaking with Quintin Marcelino who is Addgene’s... -
Editor's Choice, August 2016
TypeBlog Post...Nature. Our take home: users likely need more time to optimize and troubleshoot the system. Site Directed... -
Scientist Networking: What is an Informational Interview?
TypeBlog Post... and more open to developing a relationship. The time to develop relationships is before you need them...that is helpful. Keep in mind that this is a good time to ask personal questions about salary, opportunities... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog Post...infect only one or a few species of bacteria and sometimes, only a subset of strains of the same bacterial...researchers in the directory are volunteering their time and resources and there is a chance that an early... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog Post...play a role in the progression of cancer are often time consuming and expensive, especially when done in...functionally validated. In vitro assay allows first-time functional annotation of thousands of mutations ... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post...published in the 1990s, biologists have spent a lot of time making things glow. Chances are you’ve used a GFP...already been made, and it would be expensive and time-consuming to repeat the process with these new photoconvertible... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog Post...what they need - whether it's their first or 500th time on addgene.org. Additionally we also wanted to highlight...look and explore the new homepage. We hope every time you come to Addgene to find the plasmid or viral... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog Post...set of neurons can then be targeted with optical stimuli. The restricted light exposure to a specific area...opsin expression in their cell bodies. Optical stimulation in a different brain area can then reveal the... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post...the host plant genome. Scientists have worked to optimize this system for gene transfer, studying the stability...switching the E. coli origin of replication and codon-optimizing the Agrobacterium ORI, they achieved much higher... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog Post...be crucial for a successful application. It takes time to prepare an excellent resume…it is worth it. Read...connections to the hiring group. Read here for more on timely submission of a job application. 3) Read job descriptions...