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We narrowed to 1,076 results for: TOR

Showing: 561 - 580 of 1076 results

  1. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...have deposited their plasmids into the Addgene repository. More than 4,500 plasmids have already been deposited...aware of what Addgene can do for them and how the repository can help them share their materials. The new ...macrophages/monocytes using lentiviral expression vectors. I also used plasmids made and shared by others...carrying out my studies, and getting them from collaborators was extremely helpful, but with hindsight I ...

  2. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...pre-clinical and human trial phases.   So how does CRISPR factor into this workflow? CRISPR both speeds up the process...mutations and then test both individual and combinatorial drugs on for effectiveness prior to patient ...breast cancer and identified several candidate inhibitors of gene X in a screen. You generate a gene X ...CRISPR Guide CRISPR Plasmids and Resources CRISPR History and Development for Genome Engineering    ...

  3. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3 has been requested nearly 400 times, and... plasmids covering three different epigenetic effectors. The diversity of tools is especially exciting... subpages, our updated CRISPR Guide and CRISPR History provide a great overview of the development of ...resources: CRISPR Plasmids and Resources and CRISPR History and Development for Genome Engineering  ...

  4. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    ...through Caitlin’s blood. “I come from a family of educators, mostly elementary school, middle school, and ...school, I actually served as the student course coordinator. With the guidance of one of the faculty members...their lecture, giving them advice, [and helped] proctor exams.” These teaching experiences led Caitlin ...interview experience.” Although luck wasn’t the only factor in Caitlin landing her job. All of the hard work...

  5. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ... we’ve curated microbiology plasmids from the repository onto one handy Microbiology Resource page and...Addgene’s plasmid sharing services, as well as #happydepositors who stopped by to say hello. Needless to say...amazing work already represented in the Addgene repository (like these new Rainbow Bacterial Fluorescence...sphaeroides!) at the ASM booth. As well as being the Director of the Great Lakes Bioenergy Research Center and...

  6. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...How else can antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt ...successful cloning. In these instances, the cloning vector typically has two separate resistance cassettes...powder form, but quickly breakdown in solution. Storing aliquots at -20oC and avoiding repeated freeze/...not add them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive...

  7. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...the table below for more details.   Common Vectors Copy Number+ ori Incompatibility Group Control ...Relaxed This table defines common cloning vectors, their copy number, ori, and incompatibility groups... ori's are referred to as phagemids.   Other factors that affect plasmid copy number  Although the...affect the copy number of a plasmid, other external factors contribute as well. These considerations are especially...

  8. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over 100 entries is...data prepared. All of these questions relate to factors that you should have already recorded as good reproducible...Chemogenetics) or light (Optogenetics) to activate receptors? If you’re in the process of designing your experiment...Addgene.org Read our AAV guide Browse all  AAV vectors Find ready-to-use AAV preps ...

  9. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ... first step. Developing mentoring relationships, especially with peer mentors, to discuss your strengths...recently, I have been hearing exit survey data from postdoctoral programs in the Boston area that demonstrate...Both explicit and implicit aspects of today’s postdoctoral training can directly interfere with a seamless...

  11. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...after subtracting out non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins...the extracellular domain of the erythropoietin receptor and subsequently evaluated interactions with mutant...antibodies against MBP are commercially available. Factor Xa loves calcium, but metal-chelate matrices, not...Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies in our Hot...

  12. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...included a voltmeter on top of the battery box to monitor charge status and a disconnect switch between the... the battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down.... can enable applications where reagents must be stored at elevated temperatures for long time periods ...

  13. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ... CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over 90% in ...such as ApE or commercial workbenches such as MacVector or Benchling. Utility functions: These functions.... For this reason I generally recommend that laboratories that have already attempted several CRISPR experiments...above or, even better, first-hand knowledge from laboratories employing CRISPR on the same organism could ...CRISPRseek offers great utility and coupled with Bioconductor should be the first choice for anyone already...

  14. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ... at 230 nm. The A260/A280 ratio is used as an indicator of DNA purity. Ideally, this number should be ...Concentration (ug/ml) = A260 reading x dilution factor x 50 ug/ml This method is quick and simple and ...like a PCR product). This method also gives an indicator of DNA or RNA contamination based on the presence...protocols and tips Find the best way to elute and store your plasmid DNA Learn about the different grades...

  15. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...Tools for checking grammar and jargon Hemingway Editor  This is a free web-based tool for detecting the... saved to your Mendeley library. The library is stored in the cloud, so you can access it from anywhere...you organize and cite papers. With the Zotero Connector, papers can be saved from Firefox or Chrome browsers...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...

  16. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...University, and did postdoctoral work at Harvard Medical School and the University of Toronto. He is an author...quick turn-around time of Sanger sequencing have historically matched the needs of most molecular biology ...next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes...

  17. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...none are lost. Each Addgene library has its own depositor transformation and amplification protocol, but... a file in FASTQ format, a standard format for storing NGS reads and their corresponding sequencing quality...from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis scripts...screening, and if you have any tips, input, or stories of interest about library use, let us know in the...

  18. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...imaged together, you will need to consider the same factors as when choosing an individual fluorescent protein...previous blog post for more discussion of these factors). In addition, you will also need to choose fluorescent...infrared fluorescent proteins require biliverdin as a cofactor and so you may need to supplement your cells with...received his PhD in biophysics from UCSF in the laboratory of Ronald Vale, after which he was a fellow at...

  19. Hot Plasmids - May 2022

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...comparison to the 62-90% error rate seen with Cas9 editors. The lab believes that this system could potentially...Development of a cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang, et al, ...live cells remains a challenge due to multiple factors, including limitations of number of flourescent...

  20. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...light activation, you can’t use fluorescence to monitor processes directly affected by light. Long-term...To make luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University...Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns...
Showing: 561 - 580 of 1076 results