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We narrowed to 900 results for: TRI

Showing: 561 - 580 of 900 results
  1. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...laboratory of Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will... function in receiver cells. There are over 40 trillion cells that make up the human body and G-baToN ...
  2. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...photostable YFP to date. As such, mGold is expected to contribute to experiments that require high photostability...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth...
  3. Plasmids 101: Terminators and PolyA signals

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    Blog Post
    ...plays an important role in RNA processing and contributes to variability in RNA half-life, and ultimately... Rho-independent termination is also known as intrinsic termination, and it relies on the formation of...
  4. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...Cardiovascular and diabetes mortality determined by nutrition during parents' and grandparents' slow growth ...
  5. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...This post was contributed by guest blogger Iris Lindberg, Professor at the University of Maryland School...Cloning cells using an agarose overlay does have one tricky step. It is necessary to pour the agarose onto ...
  6. Truncated gRNAs for Regulating Gene Expression

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    Blog Post
    ...This post was contributed by guest bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss...relative nuclease efficiencies as well as to more stringent targeting rules that result in a decrease in available...
  7. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...license. If you’re currently using mVenus or mCitrine, or if existing yellow fluorescent proteins just...anterograde labeling across single synapses, a key attribute for neuronal tracing. Labeling was nontoxic and...
  8. The AAVantages of AAV in CRISPR Screens

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    Blog Post
    ...genes for drug discovery or to understand the intricacies of molecular pathways. These screens have gotten...solutions have been found to get around this restriction. The most obvious workaround is to use a multi-plasmid...
  9. Academic vs. Industry Postdocs

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    Blog Post
    ...Updated Apr 6, 2021. This post was contributed by Laura Desrochers, a postdoctoral scientist at AstraZeneca... academic lab is closely involved with clinical trials or has ties to industry, the drug discovery process...
  10. The Scientific Conference Poster Session: Tips for Success

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    Blog Post
    ...This post was contributed by Brittany L. Uhlorn, a PhD Candidate at the University of Arizona. Perhaps... next poster session! There many other tips and tricks for presenting at scientific conferences, so let...
  11. PCR Applications for Copy Number Variation Assays

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    Blog Post
    ...standard curves, and normal controls must be run in triplicate.  The nice thing about qPCR is that since it ... of reactions, it does not need to be run in triplicates. ddPCR is an extremely accurate application, ...
  12. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...are treated with a drug of interest and gRNA distribution is analyzed in the drug-resistant population...6 gRNAs per target gene, and maintaining the distribution of each gRNA within the population is key. Loss...
  13. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery ...along the illumination path. The shadows appear as stripes in the acquired image (5).  A way to get around...
  14. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...multicistronic vectors work? Scientists have "borrowed" some tricks discovered in positive single-stranded RNA viruses...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...
  15. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...This post was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how... short as 100ms (EBFP) or as long as 1 hour (mAmetrine1.2). However, for most FPs it is a few seconds ...
  16. Tips for arabidopsis transformation

    Type
    Blog Post
    ...This post was contributed by Laura Lee, a graduate student at Stanford University. Arabidopsis is a fantastic... it pretty much is, but there are some tips and tricks you can use to obtain optimum transformation efficiency...
  17. An Introduction to Adenovirus

    Type
    Blog Post
    ...noticing unusual cases of acute hepatitis in the pediatric population. Once ~400 cases had amassed in places...removal of certain adenoviral genes that apparently contribute to virulence (Tollefson et al., 1996) – cell ...
Showing: 561 - 580 of 900 results