...to expand upon the work and continue to make contributions towards this COVID-19 pandemic,” says Balazs...

...instability can cause partial loss of an ITR during routine plasmid propagation, with complete ITR loss abolishing...

...scripts with laboratories and viral vector cores routinely producing AAV. We believe that this simplified...

...GPCR based sensors provide the spatiotemporal resolution required for precise in vivo measurements of ...

...represents another leap forward for the CRISPR/Cas9 revolution!   References Streamlined Genome Engineering...

...Mentee reach goals. One of the most important contributions made by a Mentor is to help the Mentee feel ...

...in the dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25 µL of 1X dilution buffer ... carefully add the dilution buffer to the dilution plate according to the dilution scheme listed in step...Pro-Tip Prepare the viral dilutions in a separate biological safety cabinet (dilution BSC) than that used for...touching the screen. Prepare the Serial Dilution Place a 48-well dilution plate in a chilled 96-well freezer... add 5 µL of each viral sample to Dilution 1 in the 48-well dilution plate and pipette 5–10 times to mix...change tips each time. Dilution 1 (20X): 5 µL in 95 µL 1X PCR buffer (1:20) Dilution 2 (20X): 5 µL in 95 ...PCR buffer (1:400) Dilution 3 (20X): 5 µL in 95 µL 1X PCR buffer (1:8,000) Dilution 4 (20X): 5 µL in 95...

...representatives. I got to talk with a technician from Neon Therapeutics, a biotechnology company that uses neoantigen...

...-AAV1) GABA sensors Sensing levels of g-Amino butyric acid (GABA) - the primary inhibitory neurotransmitter...impacts neuronal circuits. We recently packaged a constitutive GABA sensor (see below), in addition to our ...

...tethered to dCas9, require long time scales with constitutively expressed proteins that are not inducible. ...

...advanced microscopy applications such as super resolution, electron, and expansion microscopy. hfYFP is...

...scientist, in academia, is someone who visits an institution to perform research there temporarily. The length...

... Set expectations for your productivity Set a routine and stick to it. Plan defined work hours as well...

...MG-2I, either by incubating embryos in a MG-2I solution, or injecting adult fish with the dye. Embryos...

... topics including biotech and research tools, computing performance, and randomized control trials in ...

...prevented laboratories from using these methods for routine processes like plasmid validation. At seqWell, ...

...Addgene Blog Get the Most from Your Lentiviral Transdution Using Virus in Your Research: A Primer for Beginners...

...cell culture and include a counter, lab timer, dilution calculator, and a couple of other nice features...

...condensed the system to two streamlined plasmids. The utility of these TCSs was limited by a few factors. These...

...luciferase was published in 1985, several studies utilized luciferase as a genetic reporter in plant and ...