Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 841 results for: ang

Showing: 561 - 580 of 841 results

  1. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...targeted modifications. A recent publication by Zhang et al. shows just that - using MMEJ to insert FLAG... plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016...

  2. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...stranded breaks, in which nucleotide sequences are exchanged between two similar or identical molecules of ...and animal models, their efficiency is very low, ranging from undetectable to 0.1%. In contrast, new genome...

  3. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...factor IGF2 open reading frame to relax imprinting (Zhang et al., 2017). This upregulates expression of IGF2...PMID: 23452860. PubMed Central PMCID: PMC3664290.  Zhang, Yiqun, et al. "CRISPR Cas9-guided chromatin immunoprecipitation...

  5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ... and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated methods, while technically... 29668265. PubMed Central PMCID: PMC6002863. 4. Liang, Zhen, et al. "Efficient DNA-free genome editing...

  6. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...answers hundreds of AAV questions every year on a range of issues related to AAV vectors, many from people...specifically to allow scientists to evaluate how small changes in vector administration can influence AAV expression...

  7. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... Percent of Neurons Transduced   Dorsal Root Ganglion (DRG) AAV.9 (parental) 46 +/- 0.7% AAV.PHP.S...figure 8 in Chan et al).  References 1. Chan, KY, Jang MJ, Yoo BB, et al. Engineered AAVs for efficient...

  9. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s lab) is an example of the former. In this system...While this may be difficult to resolve for a wide range of prey proteins, there are now a number of two-...

  10. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ... staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from the PAM. With this information... Other CRISPR Blog Posts Additional Resources Zhang lab Cpf1 blog post on Benchling ...

  12. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...AAV). This issue was recently circumvented by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH...as well as individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific...

  14. Viral Production at Addgene

    Type
    Blog Post
    ...digital PCR (ddPCR) titration protocol (modified from Wang et al., 2018). Briefly, 293T cells are transduced...Article 2. https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination...

  18. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ... had to overcome some hesitation to ‘approach strangers’ and learn how to communicate outside my scientific...message and to keep it short. Soon, discussing with strangers stopped feeling weird and started to be fun. I...

  20. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...watched each month, in 39 countries, and in over 54 languages. While most scientific videos were for entertainment...researchers for whom English is a second or third language (making publishing their research all the more...
Showing: 561 - 580 of 841 results