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  1. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ... staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from the PAM. With this information... Other CRISPR Blog Posts Additional Resources Zhang lab Cpf1 blog post on Benchling ...

  2. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ... had to overcome some hesitation to ‘approach strangers’ and learn how to communicate outside my scientific...message and to keep it short. Soon, discussing with strangers stopped feeling weird and started to be fun. I...

  3. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...addition, it can be advantageous to speak multiple languages - that makes communication with customers from...requirements (and maybe also the proposed salary range) should help. In smaller companies, technical support...

  4. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...watched each month, in 39 countries, and in over 54 languages. While most scientific videos were for entertainment...researchers for whom English is a second or third language (making publishing their research all the more...

  6. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...AAV). This issue was recently circumvented by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH...as well as individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific...

  10. Viral Production at Addgene

    Type
    Blog Post
    ...digital PCR (ddPCR) titration protocol (modified from Wang et al., 2018). Briefly, 293T cells are transduced...Article 2. https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination...

  14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...lamps are the best for working in this spectral range. You can also check that no filters and/or cold ...tell us about cellular events via  fluorescence changes occurring due to protein synthesis, degradation...

  15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...for researchers looking to generate long-lasting changes in cells. However, for some applications, integration...construct has the D64V mutation, a single amino acid change (from aspartic acid to valine at position 64) which...

  16. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...the nuances of the research in as technical of language as possible. Seriously. My suggested litmus test...knowledge that you have given everyone a simple plain language map of where you are going and what you expect...

  17. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...techs’ financial books.  To find personnel salary ranges, go to online job information sites (like, indeed.com...focused on research relevant objectives and their tangible resources. For example, if a dean/chair asks “...

  18. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... a wide variety of genomic edits — single-base changes, small deletions, and large insertions up to 12... B., Tran, T., … Dimidschstein, J., Fishell, G., Wang, Y. (2024). An enhancer-AAV toolbox to target and...

  19. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...palette for display (like green-magenta-blue or orange-blue rather than red-green). Even better, show ..., Ferling, I., Gazda, M. A., Hoeppner, L. H., Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour...

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...s chromatin structure undergoes significant rearrangement (such as when a stem cell initiates differentiation...researchers to avoid making unnecessary base pair changes as would be the case with ssODNs. One minor drawback...
Showing: 561 - 580 of 827 results