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We narrowed to 955 results for: cha

Showing: 561 - 580 of 955 results

  1. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb at 230 nm. The A260/A280...doesn’t tell you about contaminating proteins or chaotropic salts. Capillary electrophoresis Quantifying...fragments move through a micro- or nanofluidic channel and the sample is separated by electrophoresis....

  2. K. phaffii: Rising to the Occasion

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    Blog Post
    ... Ploidy Usually diploid, but spontaneously changes Usually haploid Usually haploid Pathology...their peroxisomes will proliferate upon a media change into methanol and peroxisomal autophagy can be ...Resources References Liu, Wei, et al., From Saccharomyces cerevisiae to human: The important gene co‑expression...

  3. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Susanna Stroik The Doudna lab’s recent work characterized a new tool for RNA targeting, with a leg up ...were compared head-to-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1...denaturing conditions, high temperatures, and other chaotropic conditions better than most FPs. The group also...

  4. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...CRISPR tools have leveraged such host-defense mechanisms to enable multiplex gene editing in a variety... fused base editors (nCas9-BE), as Cas9 is mechanistically more favored for efficient base editing. They... To do so, they harnessed the tRNA processing mechanism, which is conserved in all living organisms and...

  5. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...fusion protein binds to glutamate, conformational changes lead to an increase in fluorescence intensity from... from the inserted GFP. After characterizing iGluSnFR in vitro, showing that it is selectively activated...response than its predecessors. The detailed characterization of the new variants is available here. Upgrades...

  6. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...throughput functional characterization of specific edits to the genome. Such characterization can be done in ...The RNAi process had just been discovered, the mechanism was barely understood.” Doench’s graduate work...

  7. Resume Writing for Non-academic Science Careers

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    Blog Post
    ...non-academic one, one of the biggest changes (and perhaps challenges) is the need to present yourself using...studied astronomy or bioinformatics data, then the chance is that you have handled large data sets and are...

  8. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    Blog Post
    ... for them to be imaged in distinct fluorescent channels on the microscope. With the proliferation of fluorescent...the best protein for a given application is a challenge. To help keep you abreast of the latest fluorescent... abundant proteins, with the blue and infrared channels used for more abundant proteins or marking compartments...

  9. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...cells Blocks polypeptide synthesis at 80S; inhibits chain elongation 100-800 ug/mL Hygromycin B hygB HeLa...cells Blocks polypeptide synthesis at 80S; inhibits chain elongation. 50-500 ug/mL Puromycin pac HeLa, 293HEK...Jurkat T cells Inhibits protein synthesis; premature chain termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3...

  10. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...management, the journey can be as exciting as it is challenging. How do you prepare for this shift and navigate...experiences, and practical advice for overcoming challenges. Experimenting with leadership opportunities...'ve witnessed several colleagues do this while changing industries and positions. Some people discover...

  11. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...picked up by the sensors. You can adjust this by changing the voltage for the particular fluorophore prior...protein into your cells (visualized via the FITC channel) and you are trying to see what percentage of total...expression, using a histogram plot and MFI values to characterize your samples may allow for better interpretation...

  12. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...biological phenomenon, there’s a reasonable good chance that Addgene can help. Though it would be difficult...Kerstin Bystricky Courtney Hodges Sharon Dent Archa Fox Elaine Fuchs Kai Ge Robert Kingston Thoru ...Julie Ahringer Alexander Stark Bas van Steensel Richard Young Yi Zhang Leonard Zon  You!     Chromatin-related...

  13. Plasmids 101: Inducible Promoters

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    Blog Post
    ...IPTG, the lac repressor undergoes a conformational change that removes it from lacO sites within the promoter...there is very little crossover. The induction mechanism must also be compatible with your experimental...The Promoter Region Plasmids 101: Cre-lox Light-Switchable Two-Component Systems for E. coli Browse other...

  14. A Deep Dive into BioBuilder

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    Blog Post
    ...knew that the best days in the classroom are characterized by intense student engagement and joy, rather...development comes from the Caltech lab of Professor Richard Murray. Murray’s lab uses cellular extracts to ...collaboration has resulted in a new BioBuilder book chapter and an illustrated, downloadable classroom poster...

  15. Luminescent Imaging with Nano-lanterns

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    Blog Post
    ...imaging! These eNL constructs use the brightest characterized luciferase, NanoLuc, and its substrate furimazine...Nagai laboratory has a done a wonderful job characterizing Nano-lanterns and eNL for many different applications...eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al. also envision...

  16. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...experimental approaches. One example is CasX, characterized by the Oakes and Doudna Labs, which is both ...research and potentially treatments. Cas12b, characterized by the Zhang (Strecker et al., 2019) and Li ... relative to Cas9. As scientists continue to characterize CRISPR nucleases from a variety of sources, ...

  17. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...the presentation and Q&A session on our YouTube channel. And with no more ado, the questions!  AAV Prep...prohibitive problem? A: Re-administration is a challenge for AAV due to the development of neutralizing... in diseases which may be linked to epigenetic changes, such as multiple sclerosis?  A: Basic science ...

  18. Clear and Accessible Writing

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    Blog Post
    ...our understanding of what accessibility is would change over the next twenty years. But learn we did! ...scientific paper, you may feel it’s overwhelming or challenging. So instead, let’s focus on practicing with a...when I’m done? What are we trying to fundamentally change about their knowledge?    Here, this image is being...

  19. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ...non-coding portion of the genome. Mendenhall and Richard Myers of HudsonAlpha (where Mendenhall is also ...transcription factor. The FLAG antibody has been well characterized and the system allows you to add this same antibody... Besides being fast and efficient, what other challenges does this system help to solve? Myers: Only a...

  20. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...contributed by David Wyatt and Dale Ramsden, UNC at Chapel Hill. One advantage to using the CRISPR/Cas system...heterogeneous. There is consequently about a two-thirds chance of causing a frameshift mutation. Of some importance...the Lineberger Comprehensive Cancer Center at UNC Chapel Hill.   References Bennardo N, Gunn A, Cheng A,...
Showing: 561 - 580 of 955 results