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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 921 results for: ire;

Showing: 561 - 580 of 921 results

  1. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...exposed even one time. Doctors and researchers don’t entirely understand why some people develop the disease...one of the best ways to improve the traditionally dire prognosis for mesothelioma patients is to catch ...inhibitor) prevents the growth of new blood vessels required to sustain growing tumors. Studies have shown ...

  2. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything..., work-life negotiation, and mentorship. We can direct people to the AMA in the future and link to it ...an organizer/promoter was that I didn’t have the direct link to send people until the day of the AMA. This...

  3. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...all the steps and technical considerations are directly applicable to the rat [6]. Technical differences...Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins." Nature...Central PMCID: PMC3082132. 9.Del Bene, Filippo, and Claire Wyart. "Optogenetics: a new enlightenment age for...

  4. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...which is ideal for observing and manipulating the entire developmental cycle. A majority of studies involving...most significant advances is the sequencing of the entire axolotl genome, revealing an abundance of genes...year 2000, becoming the first plant to have its entire genome sequenced (Koornneff and Meinke, 2010). ...

  5. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... developed using yeast display technology, are directed to integrins that bind the Arginine-Glycine-Aspartate...2022). By contrast, LVDP-binding integrin α4β1 required and conferred less force, leading cells to spread...integrins, αVβ3 and αVβ5, participated but were not required for cell adhesion. The results surprised the investigators...

  6. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... the activity of the original, generated using directed evolution (Tei et al., 2023).  PLD can hydrolyze... doesn’t matter. Plus, for folks interested in directed-evolution methods, the activity-based selection... J. C., & Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian cells...

  7. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...really are selling yourself. A sales pitch that requires reading two pages or even a paragraph to get to... Simply stated, what are your aims? You could directly state your official aims, but I suggest distilling... and Structural Biology, and now serves as the Director of Student and Postdoctoral Programs at the Janelia...

  8. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...speaking early and often. Two of my jobs have indirectly resulted from someone seeing me speak in a non-interview... research, “aha” moments, and future research directions. Don’t worry if your work hasn’t been commercialized...does follow the general structure and flow of the entire session. I myself have a terrible habit of saying...

  9. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Brainbow-1.0 in that transient Cre recombination is required to prevent constant inversion of the construct...derivatives of the fluorescent proteins, which are directly trafficked to cell membranes. Membrane trafficking... Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire E, Morin X, Livet J. Neuron. 2014 Feb 5;81(3)...

  10. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...advantage to using direct epigenetic modifiers? As with any experiment, your desired outcome will determine... DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety of ...

  11. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ... in a DNA double strand break (DSB), the event required for frameshift and knock-in mutations. If one ...will generate a ssDNA break which can easily be repaired by cells with a near zero likelihood of mutation...sequencing The only way to quantify off-target effects entirely is to perform whole genome sequencing. While this...

  12. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...guide and the transposon RNA homology both help direct the fusion construct to the new genomic locus. ...indel formation than methods relying on homology-directed repair (HDR), and insertion is scarless, unlike...where those interactions occur in the genome. This requires both targeting a specific genome location and ...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    ... Is Bitter, but Its Fruit Is Sweet Time If you require a quick onset of expression, AAV may not be the...AAVs for DREADD delivery indicates ~7-21 days are required for the onset of gene expression. However, it ... As single stranded DNA viruses, AAVs normally require second strand synthesis before gene expression ...

  14. Guide to Using Pooled Libraries

    Type
    Guide
    ... and allow you to design additional hypothesis-directed experiments. As you might have guessed from their...For some libraries, plasmid DNA can be delivered directly to the cells of interest. With others, notably...

  15. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...variable region of an antibody does not recognize the entirety of the target protein: instead, it recognizes ...between the IgG antibody and scFv. Because the entire antibody molecule is not necessary for antigen ...other markers can be injected into an animal, to directly label circulating cells or other cells that are...

  16. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...antigen of interest in the given protocol. This requires use of one of the five pillars of antibody validation...intended application — here, flow cytometry — is the desired target, using one or more of the approaches outlined...relative protein expression. Its reliability will be entirely dependent on the selectivity of the antibody used...

  17. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...with two homology arms on each side flanking your desired insertion or mutation. Each arm is around 800 bp... in the HR template. Since the PAM sequence is required for successful targeting and cleavage, changing...

  18. Modular Cloning Guide

    Type
    Guide
    ...assembly step, compatible Level 0 parts are directionally assembled into a Level 1 vector creating a single...highly-tunable GPCR-based biosensors in yeast (requires MoClo-YTK parts for use). Yeast GoldenBraid Cloning...

  19. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein...each and every Addgenie, from our co-ops to our directors, we can't celebrate Addgene without recognizing...
Showing: 561 - 580 of 921 results