Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 971 results for: lars

Showing: 561 - 580 of 971 results

  1. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ... you rule out errors like uneven pipetting or irregular membrane transfer. Figure 1: A loading ...important to validate these assumptions for your particular experiment and choice of loading control. First...Ubiquitously expressed proteins like β-actin or GAPDH are popular choices for loading controls. These are often called...in many cases, you should validate it for your particular setup. We recommend starting by searching the...

  2. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...classes and resources that can provide details and vocabulary for this skill. What this post may do is help...something should be communicated is necessary in a large organization, because confirming that every person... I don’t just rely on what I know about those particular teams at that given time. I think about how previous... previous iterations of those teams resolved similar issues in the past, or how completely different teams...

  3. March for Science

    Type
    Blog Post
    ..., are limited by reality – a government cannot declare that gravity change direction and make things fly...comparison to 37% of the general public (5, 6). Similarly 90% of active research scientists believe climate... supporting it.  3) Linking scientists to one particular political party while alienating the other While...or politicians to interpret it for them. Other popular reasons to march include: General inclusion of...

  4. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...that strongly binds the glycoprotein, avidin or a similar protein, streptavidin. This strong interaction ...bait protein can even target BirA* to specific subcellular locations, such as the nuclear envelope, allowing...29169465.  Ge, Yun, et al. "Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell–Cell Interactions...smaller biotin ligase for BioID proximity labeling. Molecular biology of the cell, 27(8), 1188-1196. PubMed ...

  5. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This... enough for all applications. To resolve this, similar systems have been developed for tracking various...animals only permits visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila...making this system useful for visualization of cellular extensions, such as neurons in the fly visual ...

  6. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...accomplishment while the longer tasks will give you larger payoffs later. Don’t be afraid to accomplish some...ve also recently started using Trello to manage larger, multi-person projects. Day-to-day, flexible to-do...below): Read new Zhang Lab Paper Find plasmids similar to those in the paper Ask Mary G. about her thoughts...how much time you can possibly handle doing a particularly annoying task and then dedicating that much ...

  7. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...genes for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency...Table 2 provides a quick reference for some of the popular strains, their genotypes, and their primary use...Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic ...coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin of Replication The ...

  8. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... factors of phages) constitute one of the most popular classes of phage proteins to study. These are highly...characterizing sugars and sugar-containing molecules in molecular biology labs (for a review, see Simpson et al.... without causing issues associated with cloning large fragments. After fragmentation, repair sheared ends... and a few proteases knocked out making them particularly useful for protein production), then dilute ...

  9. AAV Production in HEK293 Cells

    Type
    Protocol
    ... Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol can...efficient means to scale-up without having to handle a large number of T-175 flasks. Workflow Timeline Day 0:... (PES) filter system, Nalgene, 565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration...the amount of each plasmid needed to have a 1:1:1 molar ratio with 2 mg total DNA per CS5 Plasmid Plasmid...determine the total μg/bp we need to achieve a 1:1:1 molar ratio of each plasmid: 2000 μg / 24,961 bp = 0.08.... You are now ready to purify your prep . The clarified supernatant can be kept overnight at 4 °C before...

  10. Water Bath Protocol

    Type
    Protocol
    ...equipment that helps bring your materials to a particular temperature, catalyze chemical reactions such...such as restriction digests needed in molecular biology, and thaw cell lines. There are many different types...properly maintain the water bath, empty and clean it regularly. Determine the amount of water needed to fill ...out after your incubation is over. Note: If you regularly use a water bath in the lab, you can leave the...previous uses. However, you will still need to regularly clean out the water bath even if you are using...

  11. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ... excited to announce the fourth edition of our popular eBook, Plasmids 101, is now available!   Plasmids...everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting tips. The...updated with twelve new sections, including our popular "Dimers and Multimers" post. It's also been re-...

  12. Centrifugation

    Type
    Protocol
    ...microfuge tubes used in labs, though some larger models can hold larger conical tubes. Depending on the centrifuge...They often spin at similar speeds to their smaller cousins, but can hold much larger containers. Ultracentrifuges...generally floor sized centrifuges that can hold larger containers, but they can also rotate at much higher...

  13. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...Our popular Antibodies 101 animation is becoming a TikTok series! Follow @addgene on TikTok for bite-...information! Link in bio. #STEM #antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene...Addgene We'll be posting these animations regularly from now until May, so follow us to get updates whenever...

  14. Lentivirus ddPCR Titration

    Type
    Protocol
    ...viruses per cellular genome: Since the cellular gene RPP30 is used as a reference to the cellular genome, ...other targets. Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) are ... lower or higher dilutions depending on their particular sample. This protocol was modified from the publication...#71205-3 Polybrene 10 mg/mL, Millipore, TR-1003-G Molecular Biology Grade Water, Hyclone, SH30538.02 ddPCR...

  16. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... the HNH and RuvC domains, which are named for similar nuclease folds found in other proteins. The C-terminal...it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg...understand how to manipulate it. Happy CRISPRing!   Molecular graphics and analyses performed with UCSF ChimeraX...DNA to read its sequence. Nature Structural & Molecular Biology, 29(4), 395–402. https://doi.org/10.1038...

  17. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...without dedifferentiating into a pluripotent stage. Cellular reprogramming The reversal of a differentiated...cells [41]. The in vivo and in vitro methods are similar except that the vectors carrying the transdifferentiation...transdifferentiation is suited for switching cell types between similar lineages, reprogramming is more versatile and universal...PMC3390875. 25. Patsch, C., et al., Generation of vascular endothelial and smooth muscle cells from human...

  18. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ... here in New England- the majority of which are solar, wind, or hydroelectric in nature. The operation... electricity will allow us to maintain these particularly energy-intensive systems in a more environmentally...cartridges can place the spent cartridge in the large cardboard box in the copy area. These spent cartridges...

  19. Call for Guest Bloggers

    Type
    Blog Post
    ...expertise through our blog. Do you have skill in a particular technique? Do you have a set of tips you give... An overview of software tools useful to any molecular biologist Tips for protein purification Replica...Recent Guest Writers Have Included: Wenning Qin, Molecular biologist with Biogen interested in applying CRISPR...

  20. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    ...fluorescent markers or protein tags, and suitable for molecular cloning. Plus, we decided to include 10 plasmids...µg of DNA in 20 µl of Tris buffer suitable for molecular biology experiments such as cloning, digestions... Learn about our other beta projects Read our Molecular Biology Reference ...
Showing: 561 - 580 of 971 results