We narrowed to 869 results for: lic

1. Plasmids 101: Gene, enhancer, and promoter trapping

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   ...contain splice acceptors. They require insertion into an intron of a gene, where they can be spliced into...

2. Cellosaurus: A Cell Line Information Database

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   ...is resistant to specific compounds, links to publication references, and cross-references with cell line...manually going through the pdfs of the 26,000 publications cited. If the information seems incomplete or...

3. Management for Scientists: What Makes a Good Manager Anyway?

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   ...include training or teaching others, research, and policy development. Every worker at every level does better...all times when making decisions, assignments and polices. Sometimes the boss has to be the boss and make...

4. Plasmids 101: Antibiotic Resistance Genes

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   ... perspective – a plasmid-containing cell must replicate the plasmid in addition to its own chromosomal...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat...

5. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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   ...cells, and has potential use for a wide range of applications in cellular biology. Van Haren J et al. Nature... quantifying multiple gene editing activities. Click here to find the ACE reporter plasmid! Martin et...

6. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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   ...biological concepts - and you have yourself a plant publicity crisis. The Arabidopsis Biological Resource Center...Ohio State University. In her work, she focuses in public engagement in science and promoting plant science...

7. Using Addgene's New Viral Service for Your Research

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   ...preparations have worked well in my lab, including in applications where we have delivered them in vivo to the ...distributing new types of materials. At the time of publication we’ve already fulfilled more than 140 orders ...

8. New and Upcoming Viral Vectors - Spring 2019

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   ...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR...

9. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

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   ...at Addgene have gone virtual!   2. Practice public speaking For almost any science career, there will... will be a time when you need to speak publicly to communicate your work. One of my 10 tips for giving...

10. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    ...these tools, which aren’t always easy to find in publications, so this is a much-needed community resource...exactly which strain you used so if they wish to replicate your experiment, they know exactly where to get...

11. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    ...reached the level of expression needed for most applications.   Figure 1: With blue light, the VVD...Ventura. BLADE activates transcription with the flick of a light switch and shuts off just as quickly....

12. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    ... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in...Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue...

13. Plasmid Cloning by PCR

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    ...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly...

14. Hot Plasmids: FRET-Based Biosensors

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    ...lymphocytes, too, making them a versatile tool for application in both neuroscience and immunology. Don’t be...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ...

15. The Importance of High Titer for AAV Transductions

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    ...you will have. When possible, use low-bind or siliconized tubes to store samples as it is more difficult...anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense ...

16. Pushing the Limits of DNA Assembly

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    ...transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new...

17. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    ...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...lines. In the meantime, be sure to browse the publication references and web links from my first post and...

18. The Power Behind NGS Plasmid Validation: seqWell

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    ...sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve...of Toronto. He is an author of 20+ scientific publications and patents.   ...

19. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    ...identified MerMAIDS from their analysis of different publicly available metagenomic data including the Tara ...demonstrates the power of combining the analysis of publicly available metagenomic datasets with biophysical...

20. Scientists Map the SARS-CoV-2-Human Interaction Network

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    ... we found in our map have an impact on virus replication. While we and our collaborators are starting ...coronavirus, one FedEx package at a time Since the publication of our paper, our group has been inundated with...