We narrowed to 862 results for: lic
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TypeBlog Post...lymphocytes, too, making them a versatile tool for application in both neuroscience and immunology. Don’t be...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ...
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Using Addgene's New Viral Service for Your Research
TypeBlog Post...preparations have worked well in my lab, including in applications where we have delivered them in vivo to the ...distributing new types of materials. At the time of publication we’ve already fulfilled more than 140 orders ... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...cells, and has potential use for a wide range of applications in cellular biology. Van Haren J et al. Nature... quantifying multiple gene editing activities. Click here to find the ACE reporter plasmid! Martin et... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...reached the level of expression needed for most applications. Figure 1: With blue light, the VVD...Ventura. BLADE activates transcription with the flick of a light switch and shuts off just as quickly.... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in...Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post...these tools, which aren’t always easy to find in publications, so this is a much-needed community resource...exactly which strain you used so if they wish to replicate your experiment, they know exactly where to get... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog Post...at Addgene have gone virtual! 2. Practice public speaking For almost any science career, there will... will be a time when you need to speak publicly to communicate your work. One of my 10 tips for giving... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post...biological concepts - and you have yourself a plant publicity crisis. The Arabidopsis Biological Resource Center...Ohio State University. In her work, she focuses in public engagement in science and promoting plant science... -
Plasmid Cloning by PCR
TypeBlog Post...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly... -
Pushing the Limits of DNA Assembly
TypeBlog Post...transformation or as a PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...lines. In the meantime, be sure to browse the publication references and web links from my first post and... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve...of Toronto. He is an author of 20+ scientific publications and patents. ... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post... we found in our map have an impact on virus replication. While we and our collaborators are starting ...coronavirus, one FedEx package at a time Since the publication of our paper, our group has been inundated with... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...identified MerMAIDS from their analysis of different publicly available metagenomic data including the Tara ...demonstrates the power of combining the analysis of publicly available metagenomic datasets with biophysical... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog Post...Collaboration and creativity are pivotal in writing publications/grants too. As described by several works (Wuchty...approaches can lead to frustration, delay of publication, or even the termination of a project.” Take ... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...funded by the public and performed by researchers who received their educations from public schools and ... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...you will have. When possible, use low-bind or siliconized tubes to store samples as it is more difficult...anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense ... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...throughout the lifespan of the fly to determine the implications of your genetic manipulations. You can produce...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ... PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers...