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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 922 results for: tro

Showing: 561 - 580 of 922 results

  1. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...a large number of requests for vectors in the “controls” category, which includes things like viral vectors...vectors in a variety of serotypes, including the retrograde AAVrg and the systemic capsid PHP.eB.   ...Patrick, M., Guerin, K., & Fan, M. (2023). Quality control for Adeno-associated viral vector production. Neuromethods...

  2. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...of symptoms in animal models, Rett syndrome is a strong translational candidate for gene replacement therapy...therapy. In 2014, RSRT brought together a strong collaboration between two gene therapy labs (Brian Kaspar...neurological disease (Sinnamon et al., 2020). They introduced a mouse MECP2 guide RNA and a hyperactive version...

  3. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...advantages. An introduction to Gateway technology The Gateway cloning method, developed by Invitrogen, is an ...an in vitro version of the integration and excision recombination reactions that take place when lambda...

  4. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...protein instead localizes to the cytoplasm? Or more troubling, what if a significant fraction of the fusion ...these compartments using CRISPR systems should strongly consider using moxFPs over standard FPs for the...Structural Biology. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment and structure...determination By Mike Lacy Conventional cryogenic electron microscopy (cryo-EM) protocols typically require...glia-specific intermediate filament expressed in astrocytes throughout the brain, making it a popular target...

  6. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE...have Kd around 70 mM and 100 mM, respectively. Introducing mutations and using potassium-binding protein...

  8. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...transcriptional regulation, as long as they can control recombinase activity. For example, post-transcriptional...methods section of our paper (1). How might one troubleshoot GSRMs that don't work as expected? The successful...the parts used to build them. We recommend using strong, directional terminators and promoters with buffer...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...the number of integration copies can be tightly controlled by titrating the transposase to transposon ratios... levels of your gene of interest, as needed. Controlling the expression levels of your gene may be particularly...splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites.,” Nat. Protoc., vol. 4, no. ...

  10. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...interaction is not covalent, later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent...2). If you’re working with purified proteins in vitro, you can even use the same tag for multiple proteins... actin filament assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled...

  11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...CRISPR/Cas9 allows us to delete sequences and introduce mutations and epitopes with unprecedented ease...in 1-2 kilobases of additional sequence being introduced. Cre-mediated excision of the selective cassette... ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc-119+ selection. https...

  12. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...and LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial...Identifying actively silenced plants Once you’ve introduced your RNAi into your organism, how do you identify...insertion of any target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert...

  13. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...happy cloning! This article was written by Susanna Stroik and Meghan Rego, with thanks to Rob Hurt for his...Plants Mediated by a Cas9 Gene Containing Multiple Introns.” Plant Communications, vol. 2, no. 2, Elsevier...Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor ...

  14. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...substance (fluorophore) that has absorbed light or electromagnetic radiation at another wavelength (Heinrichs,...Heinrichs, 2009). When a fluorophore absorbs light, electrons are elevated from a ground state to an excited state...After a brief elevation in this excited state, the electron returns to its ground state by emitting a photon...

  17. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción... aumentar el acceso global a los materiales de nuestro repositorio. Este increíble regalo permitirá que...

  20. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...not just for a better day at the office but for stronger science as well. This blog is our way of continuing...Principal Investigator. How-to guides for troubleshooting your experiments and getting better results...
Showing: 561 - 580 of 922 results