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  1. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...using the CRISPR/Cas system. Plant Methods 9:39 . https://doi.org/10.1186/1746-4811-9-39 Gao Q, Xu W, Yan...cereal genomic studies. New Phytol 223:2120–2133 . https://doi.org/10.1111/nph.15889 Gelvin SB (2003) Agrobacterium-Mediated...behind the “Gene-Jockeying” Tool. MMBR 67:16–37 . https://doi.org/10.1128/mmbr.67.1.16-37.2003 Khatodia ...Application in Improvement of Crops. Front Plant Sci 7: . https://doi.org/10.3389/fpls.2016.00506 Kwak S-Y, Lew ...nanotube carriers. Nat Nanotechnol 14:447–455 . https://doi.org/10.1038/s41565-019-0375-4 Lowder LG, Zhang...Transcriptional Regulation. Plant Physiol 169:971–985 . https://doi.org/10.1104/pp.15.00636 Ma X, Zhang X, Liu...virally delivered CRISPR–Cas9. Nat Plants 6:773–779 . https://doi.org/10.1038/s41477-020-0704-5 Santana I, Wu...
  2. CRISPR 101: Epigenetics and Editing the Epigenome

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    ...epigenome editing. Nucleic Acids Research 47:e23–e23. https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito...and enhancers. Nature Biotechnology 33:510–517 . https://doi.org/10.1038/nbt.3199 Find plasmids from this...human genome. Nature Biotechnology 35:561–568 . https://doi.org/10.1038/nbt.3853 Find plasmids from this...-MQ1 fusion protein. Nature Communications 8: . https://doi.org/10.1038/ncomms16026 Find plasmids from...in the Mammalian Genome. Cell 167:233–247.e17 . https://doi.org/10.1016/j.cell.2016.08.056 Find plasmids...fusion proteins. Nature Biotechnology 31:1137–1142 . https://doi.org/10.1038/nbt.2726 Find plasmids from this...site-specific DNA methylation. Biology Open 5:866–874 . https://doi.org/10.1242/bio.019067 Find plasmids from...
  3. A History of Genome Engineering in Popular Culture

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    ...double-strand breaks or donor DNA. Nature 576:149–157 . https://doi.org/10.1038/s41586-019-1711-4 Avery OT, MacLeod...The Journal of Experimental Medicine 79:137–158 . https://doi.org/10.1084/jem.79.2.137 Carroll D (2011) ...With Zinc-Finger Nucleases. Genetics 188:773–782 . https://doi.org/10.1534/genetics.111.131433 Doudna JA,...engineering with CRISPR-Cas9. Science 346:1258096 . https://doi.org/10.1126/science.1258096 Gaudelli NM, Komor...genomic DNA without DNA cleavage. Nature 559:E8–E8 . https://doi.org/10.1038/s41586-018-0070-x Griffith F (...Pneumococcal Types. Journal of Hygiene 27:113–159 . https://doi.org/10.1017/s0022172400031879 Hershey AD, ...BACTERIOPHAGE. The Journal of General Physiology 36:39–56 . https://doi.org/10.1085/jgp.36.1.39 Komor AC, Kim YB, ...
  4. Live and Let Dye: Self-Labeling Protein Tags

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    ...targets. Nature Communications, 7(1), Article 1. https://doi.org/10.1038/ncomms10778 Erdmann, R. S., Baguley...SNAP-tags. Cell Chemical Biology, 26(4), 584-592.e6. https://doi.org/10.1016/j.chembiol.2019.01.003 Gallagher...Reactivity. ACS Chemical Biology, 4(7), 547–556. https://doi.org/10.1021/cb900062k Gautier, A., Juillerat...Living Cells. Chemistry & Biology, 15(2), 128–136. https://doi.org/10.1016/j.chembiol.2008.01.007 Griffin...Inside Live Cells. Science, 281(5374), 269–272. https://doi.org/10.1126/science.281.5374.269 Jing, C.,... Accounts of Chemical Research, 44(9), 784–792. https://doi.org/10.1021/ar200099f Keppler, A., Gendreizig...in vivo. Nature Biotechnology, 21(1), Article 1. https://doi.org/10.1038/nbt765 Lavis, L. (2016, September...
  5. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...for codon usage tables. BMC Bioinformatics 18: . https://doi.org/10.1186/s12859-017-1793-7 Dresios J, Chappell...initiation in eukaryotes. Nat Struct Mol Biol 13:30–34 . https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan...expression. Trends in Biotechnology 22:346–353 . https://doi.org/10.1016/j.tibtech.2004.04.006 Hausser ...inhibit translation. Genome Research 23:604–615 . https://doi.org/10.1101/gr.139758.112 Mauro VP, Chappell...therapeutics. Trends in Molecular Medicine 20:604–613  https://doi.org/10.1016/j.molmed.2014.09.003 Nakamura ... year 2000. Nucleic Acids Research 28:292–292 . https://doi.org/10.1093/nar/28.1.292 Sauna ZE, Kimchi-...mutations to human disease. Nat Rev Genet 12:683–691. https://doi.org/10.1038/nrg3051 Sharp PM, Li W-H (1987...
  6. CRISPR Activation: A Practical Guide

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    ...using CRISPR–Cas9 screens. Nature 568:511–516 . https://doi.org/10.1038/s41586-019-1103-9 Bialek JK, Dunay...Transcriptional Activator Systems. PLoS ONE 11:e0158294 . https://doi.org/10.1371/journal.pone.0158294 Chari R, ...library-on-library approach. Nat Methods 12:823–826 . https://doi.org/10.1038/nmeth.3473 Chavez A, Scheiman ...transcriptional programming. Nat Methods 12:326–328 . https://doi.org/10.1038/nmeth.3312 Chavez A, Tuttle M,...activators in multiple species. Nat Methods 13:563–567 . https://doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck...Gene Repression and Activation. Cell 159:647–661 . https://doi.org/10.1016/j.cell.2014.09.029 Konermann S...engineered CRISPR-Cas9 complex. Nature 517:583–588 . https://doi.org/10.1038/nature14136 Pan J, Meyers RM, ...
  7. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...Clinical and Developmental Immunology 2012:1–15 . https://doi.org/10.1155/2012/980250  Bates A, Power CA...Prospects of Antibody Fragments. Antibodies 8:28 . https://doi.org/10.3390/antib8020028  Bird R, Hardman ...antigen-binding proteins. Science 242:423–426 . https://doi.org/10.1126/science.3140379  Bradbury ARM,...display technologies. Nat Biotechnol 29:245–254 . https://doi.org/10.1038/nbt.1791  Deal CE, Balazs AB (...infection. Current Opinion in HIV and AIDS 10:190–197 . https://doi.org/10.1097/coh.0000000000000145  Gąciarz ...intrinsically stable scFv. PLoS ONE 12:e0189964 . https://doi.org/10.1371/journal.pone.0189964  Huston JS...the National Academy of Sciences 85:5879–5883 . https://doi.org/10.1073/pnas.85.16.5879  Leavy O (2016...
  8. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...sequence-specific antimicrobials. Nat Biotechnol 32:1146–1150 . https://doi.org/10.1038/nbt.3043 Bikard D, Jiang W, Samai...CRISPR-Cas system. Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520 Citorik RJ, Mimee M...RNA-guided nucleases. Nat Biotechnol 32:1141–1145 . https://doi.org/10.1038/nbt.3011 Cobb RE, Wang Y, Zhao...Engineered CRISPR/Cas System. ACS Synth Biol 4:723–728 . https://doi.org/10.1021/sb500351f Dong C, Fontana J, Patel...transcriptional reprogramming in bacteria. Nat Commun 9: . https://doi.org/10.1038/s41467-018-04901-6 Jiang W, Bikard...CRISPR-Cas systems. Nat Biotechnol 31:233–239 . https://doi.org/10.1038/nbt.2508 Leonard SP, Perutka J...Broad-Host-Range Plasmids. ACS Synth Biol 7:1279–1290 . https://doi.org/10.1021/acssynbio.7b00399 Li Q, Sun B,...
  9. Viral Vectors 101: Optogenetic Tools

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    Blog Post
    ...Photoswitching Proteins (Vol. 2173, pp. 21–51). Springer US. https://doi.org/10.1007/978-1-0716-0755-8_2 Lehtinen, ...Frontiers in Cellular Neuroscience, 15, 778900. https://doi.org/10.3389/fncel.2021.778900 Mattis, J., ...microbial opsins. Nature Methods, 9(2), 159–172. https://doi.org/10.1038/nmeth.1808 References Abreu, N...Photoswitching Proteins (Vol. 2173, pp. 21–51). Springer US. https://doi.org/10.1007/978-1-0716-0755-8_2 Boyden, E....activity. Nature Neuroscience, 8(9), 1263–1268. https://doi.org/10.1038/nn1525 Gunaydin, L. A., Yizhar...optogenetic control. Nature Neuroscience, 13(3), 387–392. https://doi.org/10.1038/nn.2495 Marshel, J. H., Kim, Y...triggering perception. Science, 365(6453), eaaw5202. https://doi.org/10.1126/science.aaw5202 Mattis, J., Tye...
  10. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...control of CRISPR–Cas9. Nat Methods 15:924–927 . https://doi.org/10.1038/s41592-018-0178-9  Hemphill J,...Cas9 Gene Editing. J Am Chem Soc 137:5642–5645 . https://doi.org/10.1021/ja512664v  Jain PK, Ramanan V,... Protectors. Angew Chem Int Ed 55:12440–12444 . https://doi.org/10.1002/anie.201606123  Kim JH, Rege M...gene expression control. Nat Methods 16:633–639 . https://doi.org/10.1038/s41592-019-0436-5 Mathony J, Hoffmann...of CRISPR‐Cas9. Angew Chem Int Ed 58:6285–6289 . https://doi.org/10.1002/anie.201900788 Nihongaki Y, Furuhata...neuronal differentiation. Nat Methods 14:963–966 . https://doi.org/10.1038/nmeth.4430 Nihongaki Y, Kawano...optogenetic genome editing. Nat Biotechnol 33:755–760 . https://doi.org/10.1038/nbt.3245 Nihongaki Y, Yamamoto...
Showing: 21 - 30 of 246 results