We narrowed to 911 results for: TRO

...familiar with the basics of controls in experimental design, you’ll need a few controls specific to flow as an...nonspecific binding. Flow specific controls The three types of flow cytometry controls we’ll discuss are single ... may need to include additional controls like FMO or isotype controls, which we’ll describe a bit later...color control for every fluorophore you intend to use in your panel. Using single color controls On a ...your single color controls may not be accurate for your samples. The single color controls should be used...plots for a single color control. A) A flow plot showing a single color control for the APC channel. B)...likely to require FMOs. Isotypes Isotype controls are negative controls that allow you to determine what level...

...the plasmids we distribute through our quality control processes. Every plasmid we receive is rigorously...repetitive regions (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors...Addgene Blog Get 6 Tips for Analyzing and Troubleshooting DNA Sequence Learn Additional Ways to Verify...  quality control...

...optogenetics: 1) electrophysiology and 2) behavioral experiments. I’m not an electrophysiologist so I cannot...to hook up your lasers and not have the proper controls or a proper behavioral readout that allows you...behavior, you don’t necessarily need to use electrophysiology to prove that your optogenetic manipulations... (though a reviewer will probably ask for electrophysiology data too but that’s what collaborators are...not be an appropriate test.  Of course, proper controls are required and include, for example, confirmation...Carolina) that offer his constructs. Importantly, control viruses (not expressing the opsin) are also available...into the correct direction and expressed (under control of a generic promoter). A DIO opsin allows cell...

... Viruses of the Retroviridae or Retrovirus family, which includes the gamma-retrovirus and lentivirus ...using retroviral vectors in your experiments. Gamma-retroviral vectors Popular gamma-Retroviral Transfer...genome-wide experiments. Gamma-retroviral vs. lentiviral vector systems Gamma-retroviral vectors have a relatively...cells. Find tips on using gamma-retroviral vectors in our Gamma-retrovirus guide and find popular packaging... codon-optimized Cas9 under the control of Murine Stem Cell retrovirus promoter for mammalian gene knockdown...plasmids for transcriptional inhibition. Retroviral Plasmid Kit Retroviral Barcoding Library (Kit # 1000000097...multiple genes via infection with a single ecotropic or amphotropic MuLE lentivirus provides significant genetic...

...developing a new fluorescent protein tool that responds strongly to magnetic fields (Frank Hayward et al., 2024... of a magnetoresponsive fluorescent protein. Controlling an optogenetic or fluorescent tool with magnets... 2016), and some previous attempts at magnetic control of biological systems (Wheeler et al., 2016; Duret...al., 2019). But the field has sometimes seen controversy, and the interactions of organic molecules like...science in the open The York Lab operates with a strong commitment to exploration and collaboration. Andrew...says, noting that his team regularly shares troubleshooting tips and works with other labs to help them...Avants, B. W., & Robinson, J. T. (2019). Magnetic Entropy as a Proposed Gating Mechanism for Magnetogenetic...

..., VVDfast to control recruitment of an “anchor” to microtubules and iLID-SspB to control the recruitment...reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be ...with popular light-sensitive ion channels used to control activities like neuronal signaling — think of a... a few recently-deposited optogenetic tools to control the cytoskeleton and cell membrane dynamics. First...component of the cytoskeleton – actin? Actin dynamics control cell morphology, movement, and more, often driven...specific Rho GTPase/GEF pair (Ridley, 2016). Taking control of a RhoGEF is a great way to stimulate and study...

...recorded events) based on their expected size. The strong signals close to the graph’s origin mainly stem...

...rabies) naturally exhibit retrograde transport and can therefore perform retrograde tracing, but often at ...synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it enables scientists...visualize neurons that send information to a region (retrograde tracer, Fig. 1A), and another to visualize neurons...upstream from the axon terminals to the soma (retrograde tracer) (Fig. 2). Viruses as neuronal scalpels...tracers, but finding a virus that works well for retrograde tracing can be difficult. The in vivo workhorse...adeno associated virus (AAV) shows relatively low retrograde transport efficiency in most serotypes. Some ...directs high-level transgene expression, and has retrograde functionality to monitor and map neurons (Tervo...

...insertions require complicated, often costly in vitro assembly methods. A new method, REPLACR-mutagenesis...mutagenesis methods Current SDM methods rely on in vitro assembly. To make a point mutation, you design two...Trehan et al. wondered if, instead of relying on in vitro assembly, they could make the E. coli do the work...must PCR amplify the whole plasmid, which could introduce mutations. Using commercially available high-fidelity...-mutagenesis also requires making your own electrocompetent cells in which the phage recombination machinery...since you skip the steps of gel purification and in vitro recombination. Across all modifications described...Reactions 1 1 1 per fragment 1 per fragment In vitro Assembly No No Yes Yes DpnI Digestion Yes Yes ...

...mutations in dystrophin, a protein essential for proper muscle function. Without functioning dystrophin, an individual... condition known as Becker muscular dystrophy (BMD). Dystrophin’s size makes it difficult to deliver via...through NHEJ, will restore the dystrophin reading frame. gRNAs bind in intronic regions, so low-frequency ...editing partially restores dystrophin expression in a mouse model of muscular dystrophy. Science. 2015 Dec 31... CA. Correction of dystrophin expression in cells from Duchenne muscular dystrophy patients through genomic...editing for correction of dystrophin mutations that cause Duchenne muscular dystrophy. Nat Commun. 2015b Feb...Our Special Collections Pages Duchenne muscular dystrophy: The most common inherited disease The genetic...