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New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

Learn what non-model organisms are, why scientists use them, and what they can teach us.

Learn how you can use the Biobits plasmids and freeze-dried cell extracts to teach students about molecular biology.

Check out how you can use digital PCR to validate your CRISPR genome edits.

Science educator, rapper, and communicator Tom McFadden gives us SciComm tips and tell us all about science outreach company, Science with Tom.

Learn how rabies virus can be used to trace neuronal connections. AAV available at Addgene can help you with the process!

The Framework for In vivo T cell Screens (FITS) allows for in vivo CRISPR screening in T cells. Learn more about FITS in our blog post.

Find tips for hosting a great virtual lab meeting with ideas for online collaboration and space for brainstorming and discussion.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Gary McDowell, executive director of Future of Research, describes what labor laws will mean for many postdocs. TLDR - increased salary or overtime tracking.

Ever wondered how Addgene makes our viral preps? We explain it all here!

Learn how Addgene works with you to increase your work's visibility and citations through efforts to organize and curate the repository.

Some promoters are only active under specific circumstances. Here's a guide to inducible promoters, which are regulated by chemicals, light, and/or temperature.

We are excited to officially re-launch our redesigned AAV Data Hub, with easy submission and DOIs to help share science!

Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

New CRISPR pooled libraries, such as SAM, CRISPRa, and CRISPRi, offer new ways to do genome-wide functional screens.

A review of protein expression mechanisms for on, off, and tunable, control of protein expression.

We highlight some exciting new deposits in our repository of plasmids and AAVs.

With this NIH's BRAIN Initiative Grant, Addgene will provide ready-to-use recombinant antibodies and will build a crowdsourced antibody Data Hub.