We narrowed to 55 results for: ELL

Opto-nanobodies (OptoNBs) are nanobodies that can be controlled by light. Learn how you can use OptoNBs to purify proteins or to modulate cell signaling.

Take behind-the-scenes look at the isolation of a thermostable Cas9, IgnaviCas9, from Yellowstone and learn about the advantages of using a thermostable Cas9 protein.

Stochastic multicolor labeling is a popular technique to label individual cells with different colors and allows for lineage tracing of cell populations over time. Read this blog post to learn about the different systems for various organisms.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Scientists at Beam Therapeutics have found a way to improve editing in primary human cells using evolved adenine base editors called ABE8.

Learn how you can use kinase translocation reporters to monitor kinase activity at the single cell level.

Learn about the different types of stem cells in this guide. In particular, we discuss the pros and cons of using embryonic stem cells and adult stem cells and using induced pluripotent stem cells (iPSCs) as a model for regenerative medicine.

Many commonly used fluorescent proteins form dimers producing artifacts in a variety of experimental settings. Learn how to avoid these artifacts.

Learn about new plasmids at Addgene including plasmids for CRISPR, sparse cell labeling, reporting calcineurin activity, and an alternative to DNA staining dyes.

Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

Learn about the many ways you can deliver reprogramming factors to generate induced pluripotent stem cells (IPSCs).

Get tips on writing your next successful grant.