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We narrowed to 224 results for: antibody

Showing: 41 - 60 of 224 results

  1. Antibody Production

    Type
    Collection
    ...Antibodies Antibody Production Antibody Production Many antibodies distributed by Addgene have been produced... use your antibody. Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols ...Addgene's antibody production methods and quality control processes for purified recombinant antibodies. Antibodies...specific antibody in order to generate high quality preparations. Following production, all antibody preps...below. Information for a specific antibody can be found on the antibody's catalog page or by contacting help...IPI). For these antibodies, IPI will be indicated as the Depositing Lab on the antibody webpage. To learn...more about these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage...

  2. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... secondary antibodies will bind to each primary antibody.      Look for primary antibodies that have ... combination of antibodies:   Target (Rat) Primary antibody Secondary antibody SCN1A sodium...a fluorescently labeled secondary antibody binds that first antibody, then you use a microscope to see... with a mixture of primary antibodies and appropriate secondary antibodies. The general process is similar... all the primary antibodies are combined in one step and all the secondary antibodies are combined in ...block, add primary antibody to bind the target, wash, add dye-conjugated secondary antibody that recognizes...use a mixture of primary antibodies, wash, add a mixture of secondary antibodies, and image all the different...

  3. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    ...a repository for monoclonal antibodies, distributing over 3,000 antibodies and associated hybridoma cell...distributing monoclonal antibodies at cost. We distribute over 65,000 antibody units per year from our...monoclonal antibodies, which are each produced by a single B cell. To create a monoclonal antibody cell line...a monoclonal antibody against a target antigen. This contrasts with polyclonal antibodies, which are mixtures... of monoclonal antibodies obtained from serum.  Our most popular monoclonal antibodies include those ...Monoclonal Antibody Research Institute dedicated to: 1) developing new ways of generating antibodies, for example... by the Developmental Studies Hybridoma Bank. Antibodies are the most widely used class of protein-binding...

  4. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...s blog Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction...accidentally leave your antibody out on the bench overnight. Do not panic! Typically, antibodies are okay if they...light-sensitive conjugate on the antibody left exposed to light?  Has this antibody been left out, gone through...complex subjects in antibody-based protocols - but thankfully this is an Antibody 101 post, so we'll keep...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene...It’s not just the antibodies that matter when you’re prepping for your experiment - there are a number...considered as well. In this post, we’ll touch on antibody storage, buffer considerations, and give just ...

  5. Antibody Plasmid Collection

    Type
    Collection
    ...Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large proteins made by the ...about antibodies and their applications in our Antibody Guide ! Cloning and Expressing Antibodies The following... collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. Plasmid...Plasmid Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-...recombinant antibodies made from plasmids in our collection! Many of these monoclonal antibodies are produced... intruders, antibodies are also extremely useful to researchers in a lab. When an antibody binds tightly...synthetically produced recombinant antibodies, created by cloning antibody components into plasmids and expressing...

  6. Of Myc and Men

    Type
    Blog Post
    ...monoclonal antibodies. However, it was (and still is) infeasible to generate highly specific antibodies for ...monoclonal antibody with high specificity. (2) Narrow down the specific epitope that your antibody binds. ...molecular biology. And the Myc1-9E10 antibody remains a trusty antibody partner; as I write this, there are...using the same antibody developed almost 40 years ago? Well…kinda. In some cases, antibody providers are...Mary Poppins of antibodies (absolutely perfect in every way)? Unfortunately, no antibody is that perfect... resources on the Addgene blog Antibodies 101: Monoclonal Antibodies Plasmids 101: Peptide Tagging Plasmid-based...? When did the commonly used anti-c-Myc [9E10] antibody come into play? We’ll dive into those questions...

  7. All Antibodies

    Type
    Collection
    ... Antibodies All Antibodies All Antibodies Addgene distributes ready-to-use...and secondary antibodies. To learn more about antibodies and their use, visit our Antibody Guide . Browse...new antibodies to add to the collection using our Antibody Suggestion Form . Browse All Antibodies ID ... use your antibody. Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols ...ready-to-use recombinant antibodies. These monoclonal antibodies undergo application-specific validation...plasmids we use to produce antibodies are available as part of Addgene’s larger Antibody Plasmid Collection ,...pages to find antibodies in our curated collections: Tags and Other Markers : Antibodies targeting popular...

  8. Institute for Protein Innovation

    Type
    Collection
    ...Innovation Antibody Collection Glypican Antibodies Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids...well as antibody plasmids to create or express plasmid-based antibodies. The majority of antibodies in the... Antibodies Institute for Protein Innovation Antibody Collection Institute for...Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Figure courtesy of IPI Browse Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Browse Epitope Tag Antibodies IPI has developed recombinant versions of antibodies that recognize common... Other Markers Antibody Collection page to browse Addgene’s full catalog of antibodies that target epitope...

  9. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...scale production of antibodies. Problems with antibodies Conventional antibodies are a non-renewable...- HA or -MYC antibody or with an anti-HA or -MYC primary and a labeled secondary antibody. While Spaghetti...secondary antibodies. Chicken IgY RANbodies are easily detected with a labeled anti-chicken antibody. Chicken...Antibodies are a go-to tool for detecting a protein of interest in cells and tissues. Although antibody...variability in activity. No Genetic Tagging - Since antibodies are produced in animals instead of via recombinant...fluorescent dyes, HRP, etc. ) after purification. Antibodies are difficult for an individual lab to produce...alternative and renewable source of monoclonal antibodies, but they are laborious to generate and costly...

  10. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    ...community.     Why recombinant antibodies? Animal-based methods for antibody production have several drawbacks...monoclonal antibody (mAb) technology circumvented many of these issues by creating immortal antibody-producing...polyclonal or monoclonal antibodies. You can learn more about recombinant antibodies in this blog post. Hello...ensure the quality of the antibodies using quantitative assays such as antibody specific activity for each...out our Antibodies 101 blog post Learn more about plasmid-based recombinant monoclonal antibodies Resources...course not. Unfortunately, the vast majority of antibodies are supplied with no sequence information, leading...to create a much needed open-access recombinant antibody/affinity reagent resource for the neuroscience...

  11. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ... validated antibody, we recommend using antibody search engines and antibody data repositories to find... that can help: Antibody data repositories, which share validation data, and antibody search engines, ...many different antibody catalogs at the same time.  If you're looking for an antibody, especially a validated...improving antibody quality throughout research, consider checking out the Only Good Antibodies community...If you’re looking for an antibody that works for your experiment, you may be overwhelmed by options —...find an antibody for your experiment. Search engines let you easily find and compare available antibodies...and experimental data to help you decide if the antibody is a good fit for your experiment. They come in...

  12. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...the ways antibodies are. scFvs are even used to screen for new antibodies. To do this, antibody variable...When you think of antibodies, you probably think of monoclonal antibodies (mAbs). mAbs have been around...vector encoding the antibody constant domain scaffold to convert the scFv into an antibody.  The ability to...Function, and Clinical Prospects of Antibody Fragments. Antibodies 8:28 . https://doi.org/10.3390/antib8020028...Learn more about antibodies  Read about another reagent sharing platform for antibodies, the Developmental...Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies for neuroscience research from... al., 1988). scFvs are the smallest unit of an antibody molecule that can bind antigen. While they may...

  13. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...Addgene.org Addgene’s General Use Antibodies Antibody guide Antibody protocols Additional resources ...of finding a ‘good’ antibody is something we’ve all experienced. Finding an antibody that works for your... common tags, the antibodies used to detect them, and how they can fix your antibody woes.   What are...monoclonal antibodies. Below we review the most commonly available tags and their antibodies along with...Addgene blog Antibodies 101: Epitope availability Plasmids 101: Protein Tags Antibodies: Of Myc and Men...Epitope tags eliminate the need for target-specific antibodies and have been widely used across species and ... or C terminus of a protein that are bound by antibodies for the purpose of purification or detection ...

  14. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... repeatedly stripped of an antibody and reprobed with different antibodies allowing multiple targets to...binding to the antibodies during the stain. The ideal blocking buffer depends on the antibodies and labels...your primary antibody Once the membrane has been blocked, it is stained with a primary antibody against your...interest. But which antibody should you use? You will likely find several different antibody options targeting... their antibody binds to protein of the correct size. Many vendors will show that the antibody binds to...gold standard in antibody validation. This level of validation ensures that the antibody is staining the...help of secondary antibodies In order to visualize proteins on the membrane the antibodies are typically ...

  15. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...heavy-chain only antibodies in the serum of a camel and soon after found similar antibodies in llamas and...Comparison of a heavy-chain only antibody and single-domain antibody, under the commercial "Nanobody" ...conventional primary antibodies, and there are unique benefits to using sdAbs as secondary antibodies. sdAbs, however...single-domain antibodies without the need for a species that naturally produces heavy-chain only antibodies. (A)...heavy chain only antibodies) (B) Nanomice express hybrid heavy-chain only antibodies that are the source... were already on a quest for smaller forms of antibodies and had begun developing Fab fragments and scFvs...the potential for an even smaller, single-domain antibody fragment was exciting, and it wasn’t long until...

  16. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide Addgene's Antibody Plasmid Collection...Addgene blog Antibodies 101: Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...single-fluorophore-conjugated antibodies. These methods can include labeling markers with multiple proteins/antibodies, targeting... to antibodies and 'staining' to refer to small molecule dyes. Indirect labeling While antibodies used...fluorophore-conjugated antibody commercially available. In other cases, fluorophore-conjugated antibodies may exist...perk is that one primary antibody can be bound by several secondary antibodies, increasing the fluorescence...selecting the primary antibody host species, as it has to be different from the other antibodies in your panel...

  17. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...multiclonal antibodies to increase overall signal from the primary antibody.  Pro tip! If your antibodies are ... your blocking, first check your antibody spec sheet. Some antibodies work better with specific blockers...make up a fresh batch.  Antibodies We always recommend either using an antibody validated for your protein...interest or validating an antibody yourself. But even with a validated antibody, it can still take time ...10,000 for primary antibodies). You’ll want to run a protein gradient to test the antibody at the recommended...outside the detectable range of that antibody dilution. Adjust the antibody dilution until your protein dilutions... biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this range...

  18. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...recombinant antibody resource called NABOR (Neuroscience AntiBody Open Resource). The antibodies distributed...the first antibodies distributed by Addgene.  The plasmids used to generate these antibodies were based...express multiple subclasses of IgG antibody. Broad range secondary antibodies are commonly used in research...-Based Recombinant Antibodies  Plasmids 101: Secondary Nanobody Toolbox Antibodies 101: Introduction to... to Antibodies Resources on Addgene.org  Browse Addgene's recombinant antibody plasmid collection References...distributed through NABOR are recombinant antibodies expressed from plasmids that have been deposited with Addgene...off of a large collection of mouse monoclonal antibodies (mAbs) that had already been thoroughly validated...

  19. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...resources on the Addgene blog Antibodies 101: Introduction to Antibodies New Antibody Animation ...bite-sized portions of antibody concepts. Each short video answers an Antibodies 101 question, like "what...what is an antibody?" or "what is an IgE isotype?"  @addgene Ready to learn about antibodies? Our new ...new antibodies animation has all the information! Link in bio. #STEM #antibodies #molecularbiology #science...Our popular Antibodies 101 animation is becoming a TikTok series! Follow @addgene on TikTok for bite-...

  20. Addgene Launches Partnership with IPI

    Type
    Blog Post
    ...website and blog, including our antibody guide, antibody protocols, and Antibodies 101 blog series.  Stay tuned...-to-use recombinant antibodies. The first collection, a set of integrin antibodies, will be available ...s website this fall. IPI antibodies will come with access to the antibody sequences, in line with the...the rest of Addgene’s recombinant antibody collection, as well as access to IPI educational resources ...resources developed to support use of their antibody tools. Addgene also offers a growing collection of educational...
Showing: 41 - 60 of 224 results