We narrowed to 294 results for: cas9

New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Explore plant-optimized plasmids for cloning, gene expression, reporter assays, genome editing, gene silencing, and more.

An overview of CRISPR's development from a prokaryotic immune system to a valuable genome engineering tool.

Guest blogger Pooran Dewari presents results of an NgAgo survey he gave to some of the first NgAgo users. Find out how NgAgo compares to Cas9 so far.

Researchers at the Wyss Institute for Biologically Inspired Engineering explain how you can use truncated gRNAs to regulate gene expression.

Various lentiviral CRISPR-Cas9 libraries have been developed to perform genome-wide knockout screens, including the GeCKO library from Feng Zhang's lab.

Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently available in lentiviral format.

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

CRISPR plasmids for precisely editing DNA bases without inducing a DNA break.

Find prime editor plasmids for (search and replace) genome editing.

Addgene's zebrafish resources: Find plasmids for use in zebrafish.

Browse Addgene’s collection of plasmid tools, kits, and resources for use in C. elegans research. Including genome engineering, codon-optimized, biosensors and reporters, and neuroscience plasmids.

Use anti-CRISPR proteins to turn off genome editing and prevent off-target effects. In this article, read about how it works and its applications

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

CRISPR plasmids for endogenous tagging of your gene of interest.

Learn about some newly developed Cas proteins and how to choose a Cas protein based on site accessibility, specificity, and sensitivity.

Learn the basics of CRISPR ribonucleoprotein (RNP) preparation and delivery for your next gene editing experiment.

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Find some tips and tricks for using CRISPR for gene editing.

Find CRISPR plasmids to study the regulatory networks involved in retinal development.