We narrowed to 104 results for: control gRNA

An easy demethylation protocol uses CRISPR-based dCas9-directed DNA avoids the use of Tet

Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently available in lentiviral format.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Need help with the plasmid deposit process at Addgene? Consult this post for an in-depth walk through the entire process.

Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Addgene's new viral service provides ready-to-use viral preps for your research.

X-CHIME, a series of CRISPR tools developed and deposited by Arlene Sharpe's lab, allows for germline knockouts in immune cells in mice.

With many different Cas9 variants out there, it can be hard to know which one to use in your particular CRISPR experiment. This post is here to help.

A round up of guest blog posts of 2024. Thank you to our guest contributors!

Studying the biophysics of Cas9 DNA interaction, the Corn lab has developed a means to enhance CRISPR genome editing through homology directed repair.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Check out all of the plasmids featured in our hot plasmids articles from 2017.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.