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We narrowed to 101 results for: donor

Showing: 41 - 60 of 101 results

  3. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...sgRNA targeting the genomic site of interest. The donor template is supplied separately. To use this system...both plasmids are transformed in E. coli with the donor DNA for editing to take place. Then plasmids are...system contains 4 plasmids: pDonor, pTnsABC, pQCascade and pCutamp.  pDonor carries a customizable cargo...systems, such as PT7, Prha, Ptet, and Para. The pDonor plasmid pRE57-Ter with multiple terminators can...MUCICAT only needs 2 newly constructed plasmids (pDonor and pQCascade) with 4 rounds of transformation ...

  6. CRISPR Guide

    Type
    Collection
    ...specific genomic edits by copying from a DNA donor template . The donor template contains the desired sequence...out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration for...cell along with the gRNA(s) and Cas enzyme. The donor DNA contains the desired edit as well as additional...homology arms. Depending on the application, the donor DNA may be a single-stranded oligonucleotide, double-stranded...for large edits or insertions. When designing the donor template, incorporating mutations that prevent further...should alter the PAM site or target sequence in your donor DNA with a silent mutation that does not change ...recombinase or phage derived serine integrases, insert donor DNA at “landing sites” defined by relatively short...

  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...the recombinase handles the installation of the donor sequence. The latest work evolves and engineers ...site. A recombinase integrates a sequence from a donor plasmid containing a complementary site into the...

  14. Plan Your Experiment

    Type
    Collection
    ... or small tag insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that...HDR efficiency . Remember that HDR also requires donor DNA to serve as template for the repair. Once you...you settle on your gRNA, you can design a donor DNA to have the appropriate left and right homology arms...

  15. Distribution to Industry

    Type
    Collection
    ...Mutation Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation...

  19. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...actinin. When tension is high vs. low in the cell the donor and acceptor of a FRET pair on actinin will produce...
Showing: 41 - 60 of 101 results