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Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

Learn about new plasmids and viral preps available at Addgene including a yeast-based tool for protein evolution, fluorescent tools, nanobody purification, new CRISPR tools, and calcium and norepinephrine biosensors.

Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for your experiments.

Addgene is teaming up with nonprofit Seeding Labs to provide plasmids to researchers in developing countries.

Fourteen years after Cas9, CRISPR has moved beyond DNA cutting. Read Addgene's recap of CRISPR Frontiers 2026, covering programmable gene insertion, RNA-sensing cell killing, and next-gen base and prime editors.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

This blog series showcases some of the new CRISPR tools we have at Addgene each quarter.

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

Learn how you can use kinase translocation reporters to monitor kinase activity at the single cell level.

Learn about new tools in Addgene's collection!

Check out some of the new and useful tools recently deposited in the Addgene repository!

Addgene has a growing collection of plasmids that are available to industry. Learn more about the available plasmids and how to order them.

Learn about using SpyLigase for irreversible peptide-peptide ligation and magnetic cancer cell capture.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing tags such as NanoLuc, HaloTag, SmBiT, and LgBiT, useful for detecting and tracking key drug target proteins.

This post describes evoCAST, a new CRISPR-associated transposase tools optimized for genetic editing in human cells.

Recent highlights from Addgene's collection include a system to examine biosynthetic pathways, and a new tool for enriching ABE-targeted cells.

mNeonGreen is a bright green fluorescent protein. Along with mWasabi and mTFP, it can be used for traditional imaging, FRET, and multicolor imaging.

New fluorescence resonance energy transfer (FRET)-based calcium biosensors and FRET-based sensors for the intracellular imaging of transition metal ions.

Learn more about luciferases, a class of enzymes used in bioluminescence, and how they are commonly used in reporter plasmids.