...105.8 KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF 107.4 KB Goldstein Nematode: gRNA...pCRISPR PDF 106.7 KB Mendenhall & Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor ; additional...

...Bio-linker-his Cd4d3-bio-linker-6His* FLAG prey CD200-Cd4d3+4-COMP-blac-3xFLAG-6his Cd4d3+4-pentamer BasiginCd4d3...

...generation lentiviral transfer vector. Each gene has been FLAG-tagged for convenient experimental use. The plasmids...summarized in the table below:   ID Cv (Capsid) FLAG Tag Terminus 79629 (N) and 79628 (C) Cv (capsid...

...been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs (wild-type and truncation...

...amino- or carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible...

...protocol. Both plasmids 47388 and 47389 include a Flag epitope tag and an SV40 NLS at the N terminus, a...

...Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 CAG Ruby2sm-Flag (does not fluoresce) Cre dependent ...

...prokaryotes. Common Epitope Tags Tag Amino Acid Sequence FLAG DYKDDDDK HA YPYDVPDYA His HHHHHH Myc EQKLISEEDL ...

...Various Plasmids T7-lacO (lactose/IPTG inducible) Flag, His, Strep, mOCR, MBP, GFP, with and without SUMO...

...lipidation signal mScarlet-I Dorus Gadella 50057 pLYS1-FLAG-MitoGFP-HA Mitochondria MCU GFP Vamsi Mootha 49153...

...there are many types of epitope tags (eg. HA, MYC, Flag), they can be deleterious and cause aberrant protein...the genome. In this work, they use AAVS1_Puro_PGK1_3xFLAG_Twin_Strep and nuclease driven recombination ...

...intron between a fluorescent protein (FP) and a 3xFLAG tag (see construct schematic below.) After self-excision...synthetic intron, and only the fluorescent protein and 3xFLAG-tag sequences are inserted into the genome. Self-excising...repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The repair template and Cas9...

...190495 Kv2.1 K+ channel scFv [K89/34] K89/34 scFv Flag Kv2.1 K+ channel Rat Mouse 190496 Kv2.1 K+ channel...Mouse 190504 Parvalbumin scFv [L114/3] L114/3 scFv Flag Parvalbumin Rat Mouse 190505 NPY/Neuropeptide Y ...Mouse Mouse 190545 VGlut1 scFv [N28/9] N28/9 scFv Flag VGlut1 Rat Mouse 190546 Kir6.2 K+ channel scFv [...

...the US and Canada convene at ComSciCon's annual flagship workshop.  Our 2018 workshop took place in Boston...students, and we pay for travel expenses to our flagship workshop. In our view, the best possible outcome...

...reach zooxanthellae, the coral’s symbiotic dinoflagellates that are photosynthetic (Salih et al., 2000... chromoproteins could be used for mimicry and camouflage. An anemone’s red colored tentacles resemble ...

...by Paix et al. Although I was able to knock-in a 2xFLAG epitope into my gene of interest, I encountered...mutation significantly enriches for knock-in of 2x and 3xFLAG epitopes into other, non-linked loci; pha-1(e2123...

...everywhere, but resources are not. Through its flagship program, Instrumental Access, Seeding Labs partners...

...After a brief stint at the venture capital firm, Flagship Ventures, Koeris helped change Novophage's focus...

... or PreScission), epitope tag (12xHis, 2xStrep, 3xFlag, Protein A, or V5), and selection marker (KanMX...

...thrombin and TEV), and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include proteins...