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We narrowed to 242 results for: kit

Showing: 41 - 60 of 242 results

  1. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...website crisprkit.org to request complete kits or find protocols and other resources. Find CRISPRkit plasmids...-engineered fat cells fight cancer Frugal CRISPR kit for classrooms Cell-surface proteomics for neurons...chromoproteins, the researchers developed a version of the kit that uses melanin production, allowing students to..., M., Lau, M. B., et al. (2024). A frugal CRISPR kit for equitable and accessible education in gene editing... out to address this limitation by developing CRISPRkit, an inexpensive system for CRISPR experiments ...easily quantify their results.  Figure 6: CRISPRkit uses a cell-free system to carry out transcription...explore cell metabolism. Plasmid components of CRISPRkit are now available through Addgene. And educators...

  2. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...which are enzyme-based mycoplasma detection kits. These kits detect mycoplasma enzymes that are not synthesized...specificity (Degeling, 2012). Bioluminescence assay kits are available from different biotech companies which...synthesized by eukaryotic cells. First, the kit components rupture the mycoplasma and then specific enzymes...

  4. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ..., plasmid kits are avaliable for less than than it would cost to buy each plasmid in the kit individually...This describes how the plasmid or other product (kit, pooled library, etc) will be sent to you. Most of...

  5. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...available in the Sybody Generation Toolbox plasmid kit: SB_concave, SB_loop, SB_convex. These scaffolds ... This selection step involves using a commercial kit for in-vitro translation of the mRNA libraries. Each...plasmids are available in the Sybody Generation Toolbox kit. Find the Seeger lab's SARS-CoV-2 sybodies! References...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... tools that you need. The Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs...biological systems” as they predict. The pOSIP plasmid kit can be found at Addgene, so what are you waiting ...constructs, while the Patron Lab MoClo Plant Parts kit contains modules specific for plant transformation... For further reading about Nicola Patron's MoClo kit and her plant synbio research, read the Addgene interview...annealing (SSA) assay. This new Platinum Gate TALEN Kit utilizes a 4-module assembly system, which reduces...additional information on using the Platinum Gate TALEN Kit, please see the Yamamoto lab’s protocol for TALEN...specifically to improve plant transgenesis. TheGreenGate kit available at Addgene includes six individual types...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...and responsive voltage indicator ONE-GO biosensors kit Antibodies for neurodegeneration research   Optimized....1016/j.neuron.2024.08.019.  ONE-GO biosensors kit By Mike Lacy G protein-coupled receptors (GPCRs) ... of selectivity. With 10 plasmids available as a kit, plus 21 optional accessory plasmids, the collection...

  9. Plasmid Cloning by PCR

    Type
    Blog Post
    ...of the PCR reaction using a kit, such as the QIAquick PCR Purification Kit. The PCR product is now ready...

  10. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...topoisomerase (2).  These days commercially available TOPO kits provide vectors or cloning arms with overhanging...covalently linked to topoisomerase. Vectors in these kits also often have the topoisomerase site inserted ...

  11. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... the entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great ...great information on ELISA troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter...

  12. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...bacterial cultures. If you will be using a kit, follow the kit's instructions. If you want to perform plasmid...plasmid purification without using a kit, you can find a protocol for kit-free plasmid mini-prep at the bottom... , and (Link opens in a new window) Promega sell kits for isolating plasmid DNA in quantities as low as...concentration and purity click here . Top Protocol: Kit-free Alkaline Lysis Plasmid Miniprep Prepare the ...Store DNA at 4°C. Tips and FAQ Plasmid purification kits provide the fastest way to obtain a high concentration...improve the purity of plasmid DNA purified without a kit it is advisable to perform a phenol/chloroform extraction...This is included in the resuspension buffer of most kits. Reference Page Top Index...

  13. TALEN Plasmids and Kits

    Type
    Collection
    ...and Kits You may also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs...constructs with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly...human cells. Veit Hornung Musunuru/Cowan Lab TALEN Kit Kit comprised of 834 plasmids of pre-assembled trimer...Gate TALEN and TAL Effector Kit 2.0. Stephen Ekker Platinum Gate TALEN Kit Platinum TALENs assembled by...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...expression. Feng Zhang LIC TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated...assembly. Kiran Musunuru and Chad Cowan Ekker Lab TALEN Kit All possible tetramers (264) of RVDs, compatible ...

  14. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter AlcR pGGA008...AlcA) and pGGC011 (AlcR) from red flame GreenGate kit Steroid-regulated LexA promoter XVE (synthetic...

  15. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ... Kits Zinc Finger Consortium OPEN Reagents Zinc Finger Consortium: OPEN Reagents (Kit # 1000000013...section: "The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For...1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids and strains used to engineer ZFNs...Oligomerized Pool ENgineering (OPEN) method. This kit consists of 5 individually packaged plasmids and ...Detailed Information Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering... Joung Nature Protocols paper . How to Cite this Kit These plasmids were created by your colleagues. Please....2009.98. Epub 2009 Sep 17. PubMed PMID 19798082 . Kit Contents The following items will be shipped as stabs...

  17. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... can be done in-lab using commercially available kits or ordered as synthesis modifications when purchasing...choose from and you can opt to do-it-yourself with a kit or purchase it commercially synthesized with a label...

  18. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...done, through a variety of methods or commercial kits.  However, antibodies conjugated to horseradish ...that can be activated to emit light with a simple kit, and is readily available on any number of secondary...

  19. Microbiology Resources

    Type
    Collection
    ...the collection. Return to top Plasmid Kits for Microbiology Kits are sets of plasmids intended for use...Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : Creating... in these model organisms. See below for plasmid kits and other resources relevant to these organisms,...listed here. Addgene Microbiology Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence...subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid kit : Integration of DNA into prokaryotic chromosomes...chromosomes - Endy and Shearwin Labs Ralstonia-GG-Kit : Assembly of Ralstonia solanacearum species complex (Rssc...Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly of up to three transcription...

  20. Worm Expression Resources

    Type
    Collection
    ...the collection. Return to top Plasmid Kits and Collections Kits are sets of plasmids intended for use ...Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate...regarding this kit can be found at the Goldstein Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen...Browse plasmid tools, kits, and resources (such as CRISPR and synthetic biology collections) for use ...Plasmids and Resources Plasmid Tools Worm Constructs Kits and Collections Other Resources The nematode Caenorhabditis...and Cre expression plasmids. Large collections or kits are described below and not included in this table...links to protocols, can be found below: SapTrap-SEC kit for C. elegans genome engineering - Bob Goldstein...
Showing: 41 - 60 of 242 results