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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 82 results for: lox

Showing: 41 - 60 of 82 results

  1. Adenovirus Guide

    Type
    Guide
    ...contamination have been developed, such as using the Cre-lox and Flp-FRT recombination systems to excise the packaging...packaging. In helper viruses, this signal is flanked by loxP sites so they can be excised. Adenoviral plasmid...

  2. Deisseroth INTRSECT Collection

    Type
    Collection
    ...and adding recombinase recognition sequences (e.g. Lox sites, FRT sites) inside of the introns (A,D). The...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...or transgene expression. Popular uses include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing...

  4. Bacterial Expression Systems

    Type
    Collection
    ...CRISPR Plasmids and Resources page. Check our Cre-Lox Plasmids Collection for a selection of plasmids containing...Mycobacterium sp. Ashwani Kumar 134405 pBbAW4k-loxP-TT-loxP-mRFP1 Cre recombinase activity Fluorescence ...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...Promoters Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids Collection...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... involving recombination elements such as FRT or Lox sites, and provides a modular system for easier exchange...recombination events at the genetic level. Upon Cre/loxP recombination, each transgene expresses one of three...recombinase/FRT system in place of Brainbow’s Cre/loxP, allowing for simultaneous use of the two systems...

  7. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    .... 0.1% Poloxamer 188 in PBS (A) Add 500 µL of 10% Poloxamer 188 to 49.5 mL PBS 0.01% Poloxamer 188 in ...Stocks of Poloxamer 188 Poloxamer 188, 10% solution A: 0.1% in PBS: 49.5 mL PBS + 500 µl Poloxamer 188 B:... Hamilton 1X PBS pH 7.4 1X PBS-MK buffer 100X Poloxamer 188 NaCl MgCl 2 KCl Centrifugal filter units (... (B) Add 5 mL of Buffer A to 45 mL PBS 0.001% Poloxamer 188 in PBS + 200 mM NaCl (C) (formulation buffer... Cover the filter membrane with 15 mL of 0.1% Poloxamer 188 PBS and incubate for 10 min at RT Spin at ... discard the flow through. Add 15 mL of 0.01% Poloxamer 188 PBS and incubate for 10 min at RT. Spin at...discard the flow through. Add 15 mL of 0.001% Poloxamer 188 + 200mM NaCl PBS. Spin at 3000 rpm for 5 min...

  8. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...Both loxP and lox511 are recognized by Cre but lox511 sites can only recombine with other lox511 sites... the identical loxP or lox511 sites (Figure 1, bottom). Since only one loxP and lox511 site will remain...with one loxP site and one lox511 site in the same orientation, and placing the additional loxP and lox511...binds loxP sites, while FLP binds FRT sites. When a DNA sequence is flanked by target sites (floxed) in ...one wild-type pair (loxP, black arrowheads) and one mutated pair (for example- lox511, white arrowheads)...sites, not with loxP sites. Alternatively, you could use a pair of loxP sites and a pair of FRT sites and... proceed either by first utilizing the loxP sites or the lox551 sites. Regardless, the first recombination...

  9. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...allele The design for a floxed conditional allele requires you to target 2 loxP sites to intronic regions...original design for creating a conditional allele, or floxed mouse, from Yang et al. requires two gRNAs targeting... and two repair templates in order to insert two loxP sites. Since two double-strand breaks are made, ...variety of other undesired outcomes, including one loxP site insertion and one NHEJ-repaired indel, often... - 0.8 kb apart. In the example below, we’ll add loxP sites for conditional deletion of exon 6 of Syt1...homology on each side. For optimal repair, place the loxP sites precisely at the cut site for each guide. ...efficiency of Easi-CRISPR for simple knock-ins and floxed models varies from 8.5-100% of pups born, with ...

  10. AAV ddPCR Titration

    Type
    Protocol
    ...Supermix for Probes no dUTP, Bio-Rad,1863023 10% Poloxamer 188, Thermo Fisher, 24040032 Droplet generation...Reagent Preparation 1X PCR Buffer containing 0.05% Poloxamer 188: Prepare immediately before use and vortex...using. 500 µL of GeneAmp 10X Buffer 25 µL of 10% Poloxamer 188 4475 µL Molecular Biology Grade Water Procedure...

  11. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...technology (Cre/loxP) to remove the donor cassette. While this method can be effective, a loxP site is left...Grégoire and M. Kmita, “Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides...

  12. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...pAAV-mDlx-NLS-mRuby2 112677 AAV1, AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 27056 AAVrg pAAV-Ef1a-DIO EYFP ... mCherry 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 50457 AAV2 pAAV-hSyn-DIO-EGFP ...

  14. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...GABAergic interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg): The...)-mCherry (26976-AAV5) pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5) Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE...

  15. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... of gene-specific 1 kb homology arms flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred vector...Then, if necessary for subsequent experiments, the floxed neoR gene is removed via infection with adeno-cre...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Lab used this backbone to target a CAG-driven loxP-stop-loxP cassette into the Rosa26 locus, allowing for...Commonly used sequences, including fluorescent tags, a floxed Cbr-unc-119 selectable marker, and “connectors”...
Showing: 41 - 60 of 82 results