We narrowed to 98 results for: sgRNA

An overview of viral applications spanning from basic research science to clinical therapies.

Learn about new plasmids including optogenetic control of synaptic transmission, acoustic reporter genes, miRNA reporter vectors, and CRISPR-Cas9 delivery for in vivo screening.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Learn how you can use Synthego's ICE tool to analyze your CRISPR genome editing experiments.

An all-in-one CRISPR/Cas9 Multiplex Genome Engineering System for transfecting and editing cell lines.

Learn about TnpB, a small genome editor with a function similar to CRISPR systems!

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

This post highlights ways to overcome the AAV size limitation to deliver CRISPR editing tools to cells.

Use these CRISPR base editing reporters to track base editing events in real-time and enrich for base edited cells.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

Learn about the latest CRISPR plasmids deposited at Addgene such as an E. coli genome-wide CRISPR inhibition library, SECURE base editors, and Nme2Cas9.

Review of off-target effects in CRISPR experiments. Topics covered include: measuring, preventing, and controlling for off-target events.

With the limitations of qRT-PCR based COVID-19 testing, scientists develop CRISPR-based diagnostic tools for COVID-19.

Learn about the latest CRISPR plasmids deposited at Addgene including plasmids for CRISPRi in Burkholderi, DNA and RNA editing, epitope tagging, and Cas9/gRNA ribonucleoprotein delivery.

New CRISPR pooled libraries from the Broad Institute allow you to specifically target the mouse and human kinomes in your next screening experiment.

Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids.

Learn about the latest CRISPR plasmids deposited at Addgene including plasmids for base editing, controlling gene expression in yeast and Caulobacter crescentus, multiplexed gRNA for human pluripotent stem cells, CRISPR-based DNA demethylation.

Various lentiviral CRISPR-Cas9 libraries have been developed to perform genome-wide knockout screens, including the GeCKO library from Feng Zhang's lab.

Learn about the pros and cons of biased and unbiased CRISPR genotyping methods.