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Showing: 291 - 300 of 556 results

  1. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-CASPR/Neurexin IV [K65A/2R] CASPR/Neurexin IV Mouse IgG2a 206606 Anti-CASPR2 [K67/11R] CASPR2 Human...177445 Anti-CASPR/Neurexin IV [K65/35R] CASPR/Neurexin IV Rat Mouse IgG2a 177446 Anti-CASPR2 [K67/25R] ...219448 Anti-CASPR/Neurexin IV [K65A/30R] CASPR/Neurexin IV Rat Mouse IgG2a 219449 Anti-CASPR/Neurexin IV...SAP97 Rat Mouse IgG2a 128625 Anti-CASPR/Neurexin IV [K66/38R] CASPR/Neurexin IV Rat Mouse IgG2a 128626...VAPA Rat Mouse IgG2a 199403 Anti-CASPR/Neurexin IV [K65/35R-2b] CASPR/Neurexin IV Rat Mouse IgG2b 199404...external) Human Mouse IgG1 206683 Anti-CASPR/Neurexin IV [K65/35R-1] CASPR/Neurexin IV Rat Mouse IgG1 206684...K28/77R] PSD-95 Human Mouse IgG2a 220381 Caspr [K65/31R] Caspr Rat Mouse IgG2a 220382 KChIP3 K+ channel...

  3. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...recent blog post on CRISPR antimicrobials to learn how scientists are using CRISPR to fight bacteria. ...from getting sick can be helpful in preventing the spread of antibiotic resistant bacteria from one person...For more information and tips on preventing the spread of antibiotic resistant bacteria, check out this...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...Educational Resource Top Five by Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide... What is a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go! How to ...primers to help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the ...Addgene blog Twenty Years of Addgene Sharing: CRISPRTwenty Years of Sharing: Addgene's Viral Vector Service...

  5. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another popular targeted gene deactivation...developed, relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality sequenced zebrafish...systems, and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite... Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection Read our molecular biology reference...

  6. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...organism.   It’s not an overstatement to say that CRISPR/Cas9 editing is one of the key tools now providing...to mate with and if she decides to raise their offspring or leave them to their own devices. Chytrid fungi...clutch of eggs? Propagation is thus quite difficult. CRISPR genome editing in squid is possible nonetheless...reptiles at all. But new success is coming with CRISPR/Cas in lizards. The Menke Lab was able to make ...

  7. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...technique is also highly relevant in this age of CRISPR; site-directed mutagenesis generally applies to...DNA through homology-directed repair (HDR) of a CRISPR/Cas9 induced double-stranded break. This site-directed...a similar approach can be used to identify Cas9/CRISPR-induced genome modifications. Occasionally, multimerization...More about Plasmids in Our Plasmids 101 Series Use CRISPR to Generate Specific Genomic Mutations Resources...

  8. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...increasing the size of the AAV donor template for CRISPR-mediated homology directed repairs (HDR). Split...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to ...single step and serve as donors for two consecutive CRISPR-mediated HDR events which results in the integration....org/10.1172/jci34316 Bak RO, Porteus MH (2017) CRISPR-Mediated Integration of Large Gene Cassettes Using...

  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ... receptor interactions of a serotype can affect spread from the injection site  (Kanaan et al., 2017),... al., 2020). For example, AAV1 and AAV9 tend to spread farther from the injection site than AAV2 (Watakabe...serotype you’ve picked, since that can affect the spread of the virus.  A better way to define high titer...AAV Capsids Improve Transduction and Volumetric Spread in the CNS. Molecular Therapy - Nucleic Acids, ...

  10. COVID-19 Resources

    Type
    Collection
    ...Lab Cas12a plasmids Addgene's full CRISPR guide and summary of CRISPR pooled libraries . FlipGFP-based ...coronavirus - Enabling coronavirus detection using CRISPR-Cas13: Open-access SHERLOCK research protocols ...develop the method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has... of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics: SARS-CoV-2 DETECTR (Link opens in ...now available from Ray Owens' lab. Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and...181(4):865-876.e12. (Link opens in a new window) CRISPR tools and reporters now available from Stanley ...Collection Science Journals Coronavirus Collection Springer Nature SARS-CoV-2 and COVID-19 Articles Wiley ...
Showing: 291 - 300 of 556 results