We narrowed to 911 results for: TRO

...CRISPR/Cas9 to cells for genome editing, and introducing inducible systems into cells, among many other...Desfarges & Ciuffi, 2012). One family of viruses, retroviridae, require integration for their life cycle to... during production (read our blog post on the retroviral life cycle for a more detailed description). ...concerning in clinical settings, as evidenced by retroviral gene therapy trials for X-linked severe combined...immunodeficiency virus type 1 integrase mutants retain in vitro integrase activity yet fail to integrate viral DNA...lessons from provirus and non-integrated DNA. Retrovirology, 1, 13. https://doi.org/10.1186/1742-4690-1-...resources on the Addgene blog Viral Vectors 101: The Retroviral Life Cycle Quick Guide to All Things Lentivirus...

...field of view. Tetbow can be introduced in vivo either by electroporation of plasmids, or using AAV vectors...type of cell labeling technique involves the introduction of a transgene construct containing fluorescent...cells, the expression level of these XFP is not strong enough for all applications. To resolve this, similar...use in Drosophila melanogaster and is under the control of Flp recombinase. The XFPs in Flybow are membrane-tethered...entire organism or a specific tissue. XFPs are introduced using a plasmid construct called Zebrabow-GateDest...Additional resources on the Addgene blog Read an introduction to live cell imaging Learn more about the origin...

...Engineering Using CRISPR/Cas Systems has expanded out of control. To further consolidate these FAQs, Le Cong worked...gRNAs with the Cas9 nickase which will together introduce cleavage of the target site with efficiency similar...on each side flanking the change you'd like to introduce. We use Ultramer oligo from IDT (non-PAGE purified...although this might still be okay if you are introducing a selection marker gene. The homology arms should...ideally less than 10 bp away if possible. For introducing small mutations (<50 bp) or a single-point mutation...around 100–150 bp total homology. The mutation is introduced in the middle, giving 50–75 bp homology arms....double digestion. As a successful insertion will destroy the BbsI sites, a double digest should discriminate...

...mimic aspects of organ morphology and physiology in vitro and have emerged as a powerful model for understanding...for doing gene targeting, reporter generation, controllable gene knockouts, and inducible gene inactivation...design. B) Strategy for leak-free transcriptional control. C) Iterative transduction workflow overview. Image...SL-insensitive fluorophore (LSSmOrange) as an internal control and a SL-sensitive fluorophore (circularly permuted...the cpGFP without altering that of the internal control (Fig 1). Because this method relies on the fluorescence...-500nM range) and a high specificity for SLs in vitro, and they further demonstrated that when tested ...

...system along with precisely truncated attP sites to control recombination of the gene construct. A predictable...gene. Effector genes, any gene that you want to control the expression of using the SPARC system. The effector...rest of the SPARC components and expression is controlled by the upstream recombination outcome. Find ...Find SPARC plasmids at Addgene To more precisely control the amount of labeling, you can alter the attP site...effector position, as well as to optogenetically control the depolarization of R2 neurons using CsChrimson...genome engineering in flies Learn more about controlling gene expression in flies Resources on Addgene.org...

...scientists and plasmid lovers - serves as an introduction to plasmids. Plasmids 101 will provide you with...plasmids contain an origin of replication (which controls the host range and copy number of the plasmid)...the lab are usually artificial and designed to introduce foreign DNA into another cell. Minimally, lab-... that scientists can purify and study it in a controlled setting. Read more: Plasmids 101: Protein Tags...environment Produce enzymes that will make specific, controlled changes to an organism’s genome (genome engineering...guide also contains multiple protocols and troubleshooting tips to make plasmid usage as simple and straightforward...

... Many thanks to our guest blogger Jon Backstrom. Jon Backstrom, Ph.D is a biochemist in the Vanderbilt...This post was contributed by guest blogger Jon Backstrom, a biochemist in the Vanderbilt Eye Institute ...oligomerization of biotinylated proteins on avidin can be controlled easier than formation of GST dimers. Avi-Tag ... of added biotins per molecule cannot be well-controlled. The lack of site selectivity typically diminishes...relatively low efficiency of biotinylation, at least in vitro. Our solution was to exploit the best of both worlds...

...activity in the brain was measured with implanted electrodes. The Archon1 voltage reporter allows a way to...cre-dependent version at Addgene! Fluorescent protein and control AAV These fluorescent protein expression vectors...interneurons. These vectors can also be used as the control vectors for experiments encoding a fluorescent-... Addgene in the next 6 months, pending quality control. For an estimate on availability date or to be ... and serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472  AAV5, AAV8 ...Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST Chemogenetics Plasmid Serotype Name ...

...exhibit profound differences in tropism between cell types (astrocytes, microglia, neurons, etc.) and ...can not recombine with each other. When Cre is introduced to the system it binds one pair of lox sites,...your cells.  Some promoters, such as CAG are very strong and may require less viral particles or relatively...or glial fibrillary acidic protein (GFAP) for astrocytes it is important to test it in the brain region... S (2013) Analysis of Transduction Efficiency, Tropism and Axonal Transport of AAV Serotypes 1, 2, 5, ...and 5 Display Differential Efficiency and Cell Tropism after Delivery to Different Regions of the Central...

... inserted GFP. After characterizing iGluSnFR in vitro, showing that it is selectively activated by glutamate...higher expression levels in bacteria and produces stronger fluorescent signals upon sensing glutamate in ...variants differing in kinetics, affinity, and ∆F/F0. Introduce the two variants in three different membrane-targeting...iGluSnFR3 have higher expression levels and produce stronger fluorescent signals upon sensing glutamate using...addition to the original PDGFR TMD, the researchers introduce iGluSnFR3 in two additional anchoring domains ...induction to express soluble protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression...