We narrowed to 922 results for: POR

1. Tips for a 1st time AAV user (by a Rookie AAV user)

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   Blog Post

   ...virus from the cell pellet alone. If yield is important, or if the viral particles of the AAV serotype...

2. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   ...improvement in off-target:on-target editing ratio. Importantly, the observed off-target loci displayed a broad...

3. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ... mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled...

4. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   ... Signaling and Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody...

5. 10 Basic tips for mammalian cell culture

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   ...passage are too young and should not be used for important transfection experiments right after they’ve been...

6. Plasmids 101: Protein Expression

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   ...expression. Despite their ease of use, it is important to note that bacteria usually cannot produce functional...

7. AAV Vector Quality Control: Going the Extra Mile with NGS

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   ...production process? These questions are especially important when producing multiple vectors in parallel because...

8. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   ...community, will soon expand to include several other important pathways. We invite feedback and contributions...

9. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...Researchers from a variety of institutions recently reported their inability to recapitulate the results of...

10. Plasmids 101: Optimizing Plasmid Yields

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    ... in copy number, the number of plasmid copies supported within each bacterial cell in your culture. Copy...

11. Light Sheet Fluorescence Microscopy

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    ...addition, the use of viruses allows much better spatiotemporal control over labeling methods as compared to...

12. Tips for Using BLAST to Verify Plasmids

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    ...you have used blastn to determine the reliable portion of a Sanger sequencing result and noted any potential...

13. Which Fluorescent Protein Should I Use?

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    ...temperature). pH Stability: This parameter is important if you are planning to express the FP in acidic...

14. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post

    ...& Kappes, J. C. (1997). Functional RT and IN incorporated into HIV-1 particles independently of the Gag...

15. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ... enough for efficient library performance, an important criterion for probing a limited number of cells...

16. CRISPR 101: RNA Editing with Cas13

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    ...26 of a 30 nt target sequence. For the editing portion of the protein, they examined ADAR1 and ADAR2, ...

17. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    Blog Post

    ...self-promotion, and personality is playing an increasingly important role in science. The science produced by the quieter...

18. DNA Purification Without a Kit

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    ...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou...

19. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post

    ... let us know! Contact our scientific technical support at [email protected] and let us help.  Check back...

20. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post

    ... for antibodies that bind to the extracellular portion of a protein. B) Permeabilizing with the selective...

21. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...concept, they tested a lux-mediated bioluminescence reporter in vivo in the absence of selective pressure and...

22. Plasmids 101: Codon usage bias

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    ...isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID: 15678157. PubMed...

23. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ... Screens Before diving into the results, it’s important to note that in Morgens et al., guides were considered...

24. Neuronal labeling with Spaghetti Monster

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    ... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal...

25. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...Hu et al., 2018).  In 2020, the Kleinstiver lab reported the development of near-PAMless Cas9 variants....

26. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ...The GPI-Linked Protein LY6A Drives AAV-PHP. B Transport across the Blood-Brain Barrier." Molecular Therapy...

27. Tips for CRISPR Gene Editing in Mice

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    ...expressing Cas9 and single guided RNA." Scientific reports 3 (2013). PubMed PMID: 24284873. PubMed Central...

28. Antibodies 101: Beyond Surface Labeling

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    ...bromodeoxyuridine (BrdU) can be used. These dyes are incorporated into the DNA during DNA synthesis and then diluted...

29. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ...primers for PCR amplification of fragments to incorporate such regions of homology. This video gives a ...

30. A Control for All Seasons

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    ...binding  Primary antibody specificity is further supported by a pre-immune serum or isotype-specific control...