We narrowed to 922 results for: Por

1. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   ...different titers are measured. A TCID50 measurement reports an infectious titer, meaning the concentration ...

2. Antibodies 101: Flow Compensation

   Type

   Blog Post

   ...change your compensation values during the analysis portion of your experiment. So, as long as you are able...

3. Common Injection Routes in Mice

   Type

   Blog Post

   ...muscle groups (usually the thigh), though it is important to avoid injection into the sciatic nerve. Mice...

4. Of Myc and Men

   Type

   Blog Post

   ... can find it in likely mean it will remain an important tool for the foreseeable future. These newer findings...

5. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   ... mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled...

6. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   ... lab has created a novel nanocompartment for transporting proteins in yeast by using murine polyomavirus...

7. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   ...virus from the cell pellet alone. If yield is important, or if the viral particles of the AAV serotype...

8. Addgene's Cancer Collection Pages Connect the Research Community

   Type

   Blog Post

   ...community, will soon expand to include several other important pathways. We invite feedback and contributions...

9. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   ...Researchers from a variety of institutions recently reported their inability to recapitulate the results of...

10. Plasmids 101: Optimizing Plasmid Yields

    Type

    Blog Post

    ... in copy number, the number of plasmid copies supported within each bacterial cell in your culture. Copy...

11. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    ...addition, the use of viruses allows much better spatiotemporal control over labeling methods as compared to...

12. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    ...you have used blastn to determine the reliable portion of a Sanger sequencing result and noted any potential...

13. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    ...temperature). pH Stability: This parameter is important if you are planning to express the FP in acidic...

14. CRISPR 101: RNA Editing with Cas13

    Type

    Blog Post

    ...26 of a 30 nt target sequence. For the editing portion of the protein, they examined ADAR1 and ADAR2, ...

15. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    ...self-promotion, and personality is playing an increasingly important role in science. The science produced by the quieter...

16. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    ... for antibodies that bind to the extracellular portion of a protein. B) Permeabilizing with the selective...

17. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    ...& Kappes, J. C. (1997). Functional RT and IN incorporated into HIV-1 particles independently of the Gag...

18. DNA Purification Without a Kit

    Type

    Blog Post

    ...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou...

19. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    ... let us know! Contact our scientific technical support at [email protected] and let us help.  Check back...

20. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    ... enough for efficient library performance, an important criterion for probing a limited number of cells...

21. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    ...The GPI-Linked Protein LY6A Drives AAV-PHP. B Transport across the Blood-Brain Barrier." Molecular Therapy...

22. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    ...Hu et al., 2018).  In 2020, the Kleinstiver lab reported the development of near-PAMless Cas9 variants....

23. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    ... Screens Before diving into the results, it’s important to note that in Morgens et al., guides were considered...

24. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    ... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal...

25. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    ...concept, they tested a lux-mediated bioluminescence reporter in vivo in the absence of selective pressure and...

26. Plasmids 101: Codon usage bias

    Type

    Blog Post

    ...isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID: 15678157. PubMed...

27. Tips for CRISPR Gene Editing in Mice

    Type

    Blog Post

    ...expressing Cas9 and single guided RNA." Scientific reports 3 (2013). PubMed PMID: 24284873. PubMed Central...

28. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    ...bromodeoxyuridine (BrdU) can be used. These dyes are incorporated into the DNA during DNA synthesis and then diluted...

29. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    ...primers for PCR amplification of fragments to incorporate such regions of homology. This video gives a ...

30. A Control for All Seasons

    Type

    Blog Post

    ...binding  Primary antibody specificity is further supported by a pre-immune serum or isotype-specific control...