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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,084 results for: ANT;

Showing: 581 - 600 of 1084 results

  1. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...other professors at the University of Barcelona wanted to develop strategies to make our classes more ...thinking about how to solve this problem. I didn’t want to face another group of sleepy students at 8 a....engaging card game could help me get the engagement I wanted. I therefore started planning a game that would...following lessons. And let me tell you more, they had brilliant ideas on how to solve many therapeutic and pre-clinical...educational nature of this material are welcome and if you want to receive our modifiable powerpoint file, please...University of Barcelona. He is also founder and consultant at FRESCI particularly interested in advanced...

  2. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...us take comfort in the fact that it’s often not quantity, but quality that really matters. Well, it turns...another example, neuronal gene expression by an anterograde AAV was shown to drop off drastically with lower...concentrating your AAV above your desired titer and want to dilute it, err on the side of caution when adding...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...PMC2574934. 3. Zingg, Brian, et al. "AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular...

  3. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...Imaging by Coat protein Knock-off) instead takes advantage of the ribosome’s movement. When the ribosome ... reporter. Delayed translation is known to be important in development, and Halstead et al. used TRICK...mRNA and keeps it in a single field of view for constant tracking. You can then directly monitor multiple...that can recruit GFP fused to the SunTag-specific antibody scFv. Each time the reporter is translated, the...second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing...and 3’ UTR. Before beginning experiments, it’s important to verify that these changes do not impact translation...

  4. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...This post was contributed by guest bloggers Samantha Zyontz and Neil Thompson from the MIT Sloan School...pulled the journals from 2005 to 2015 in areas most relevant to plasmids: Genetics & Heredity, Cell Biology...after controlling for journal. And so, while our quantitative analysis cannot rule out all other explanations...along the way. Many thanks to our guest bloggers, Samantha Zyontz and Neil Thompson. Special thanks to Addgenies...for helping compile the data used in this work. Samantha Zyontz is a Ph.D. candidate at the MIT Sloan School...ssrn.com/author=1232466.     Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT...

  5. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...-AAV-syn-jGCaMP7c variant 1513-WPRE (105321-AAV1) pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE (105322-...vectors to more serotypes. We currently package four variants of these biosensors, which are optimized for specific...relative to baseline fluorescence, as the GCaMP7f variant, and is approximately 5-fold brighter than GCaMP6s...GCaMP6s. jGCaMP7f is the fastest responding variant. jGCaMP7b exhibits the brightest resting fluorescence...fluorescence from inactive neurons is reduced. This variant has an approximately 12-fold increase in brightness...

  6. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    ... mAID and expressing the essential plant protein TIR1 in non-plant cells, which interacts with the cells...Cecchetelli Auxin Inducible Degradation (AID) uses plant hormones called auxins to conditionally and reversibly...a degron, a domain that induces degradation. In plants, auxin binds to TIR1 of the SCF-TIR1 ubiquitin ...engineering SpCas9 Article contributed by Shreya Vedantam CRISPR/Cas9 is widely used genome editing tool...versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that were...

  7. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...people use is the built-in whiteboard, in which participants can contribute and sketch on the board without...control panel on the top of the screen and every participant get to annotate things as they come up in their...time breaks up the monologue a little and gives participants something else to focus on. Use collaborative... creativity are pivotal in writing publications/grants too. As described by several works (Wuchty et al...fades away the longer we are on it – so it is important to take breaks. After 1 hour (or another designated...be done randomly or manually assigning which participants to group together. Breakout Rooms can be used...

  8. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...physiologically-relevant systems, to catch all the important interactions. It’s also important to determine...speak, with collaborators in Paris and New York. We want YOU to join the fight against coronavirus We are... a lot of background in our study - so you might want to be careful with these clones (and help us figure...been quite the administrative headache receiving constant email requests and tracking their fulfillment ...

  9. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...time, he is pursuing a plant engineering project at BosLab. Once he optimizes plant cell culture and transgenesis...transgenesis he wants to share his knowledge with the community by teaching a DIY plant engineering workshop...and to see old pictures of the lab!). By 2016, we wanted to be taken seriously so we made it official by...ecology but she became a member at BosLab because she wants to engineer microbes. We’ve ordered a few yeast ...

  10. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...expression and largely defines the amount of recombinant protein that will be produced. Many common promoters...and steroid regulated promoters commonly used in plant research. Promoter Subtype Promoter Example Activator...promoter is right for me? Here’s a list of some important variables to consider when choosing an inducible...various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique promoters and there is very...’re expressing a gene that may be toxic, it’s important that your inducible promoter not be too leaky....low leakiness. Inducibility level How much do you want to induce transcription of your target gene? The...

  11. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...diverse libraries of genetic variants. Several methods for generating mutant libraries exist, but none provide...provides a new approach for generating novel genetic variants in bacteria. Read on to learn about the key components...Assisted Continuous Evolution (PACE) method requires constant turbidity of the culture, so bacteria must be ...novel genotypes that result in a spectinomycin resistant phenotype in bacteria. While there are many known... feature which they used to generate bacteria resistant to both spectinomycin and streptomycin, but could...

  12. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...scientists to both quantify Cas9 activity and sgRNA efficiency as well as enrich for mutants through the presence... created CRISPR fluorescent reporter assays to quantify targeted Cas9 cleavage, and repair through homology... in 2017 to enrich and identify desired CRISPR mutants (Wen et al., 2018). SRIRACCHA uses the Piggybac...presence of a fluorescent reporter. Lastly, once a mutant is identified, the PB transposase can easily and...effective gRNA and enrich cells for your CRISPR mutant of interest, give these methods a try.  References...

  13. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...Plasmids 101 posts here. Now that we have covered antibiotic selection here at Plasmids 101, we can talk about...amino acids (called the α-peptide) to a lacZΔM15-mutant bacterial cell in trans complements the mutation...they should be. Don't rush the process: It is important to give your plates enough time for any intact...identify colonies that not only have your plasmid (antibiotic resistance), but also confirm those plasmids ...α-peptide DNA disruption in the blue-white screen. Antibiotic selection is also used in conjunction with this...select for plasmid-containing cells without using antibiotic resistance. These rely on cell lines susceptible...

  14. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...considerations. First, it is important to establish the type of audience you want to reach with your paper... your paper to your preferred  journal, it is important to check the journal’s guidelines to authors and...catalogue numbers and manufacturers’ details for antibodies, plasmids (like your Addgene plasmid #), and ...an identifier (a bit like a GenBank ID) to all antibodies, animal models, and software tools in the Materials...journals and their scientific communities aim to significantly improve the reproducibility and reliability ...

  15. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ... ask” once you have found someone you admire and want to learn from. Check out Joanne's Reddit AMA Who... colleagues that have the training or skills you want to learn. Mentors can be senior to you or peers ...mentors from your current organization but also important to build relationships outside your organization...confidential topics Broaden your network Take advantage of formal programs A formal mentoring program ...mentoring circle. This type of program has many advantages and I am seeing a lot of success at universities...feedback. The best thing to do to make your mentor want to keep working with you is report back with progress...

  16. dTAG - You're it!

    Type
    Blog Post
    ...transcription factors with important roles in Multiple Myeloma), it is important to ensure lack of degradation...Remarkably, small molecule degraders provide distinct advantages over pharmacological inhibitors including rapidly... are happy to share aliquots of dTAG-13 in the meantime, please address your requests for dTAG molecules... chimeras For each protein-of-interest, it is important to determine which terminus can accommodate the...context. Immunoblotting is recommended, while quantitative proteomics experiments will provide the best...

  17. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...on the conversion of non-malignant forms of cancer to invasive and malignant forms and noticed that myoepithelial...stromal cell modifications, stromal cells are also constantly communicating with a tumor, proving once again...malignancy but also incite different organs to become malignant. Exosomes also contain miRNA (micro RNA) and other... case, exosomes may transport miRNA from drug-resistant cells to sensitive ones. Though this process is..., the microenvironment backstage play just as important of a role if not more.  Many thanks to our guest...

  18. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...high-intensity light for activation which requires implantation of fiber-optic cables into the brain. ...information about parental channelrhodopsins to create recombinant channelrhodopsin sequences. The model first removed...performed well in a tissue culture dish, that doesn’t guarantee their success in vivo. Therefore, the Arnold and...fiber-optic cannulas were still stereotaxically implanted into the mice’s brains so that light could reach...into a hole in their cage. Since dopamine is an important neurotransmitter that promotes reward-motivated... brain may still require fiber-optic cannula implantation, but altering ChRgers so they can be activated...

  19. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...Accelerate Research Discoveries) Challenge provides grants for rare disease research. This year is our fourth... and induce their effector functions. It’s an important process that we need to understand so that we ...source of IL-36 cytokines in the skin making it important to study what is happening within the keratinocytes...genes from the X chromosome are expressed at significantly reduced levels in women with TS (Trolle et al...nucleus,” says Dr. Terry Hébert, a professor and assistant dean of biomedical science education at McGill.... So far, there have been thirty GNB1 missense variants identified from 58 individuals with GNB1 disorder...

  20. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... nuclease-resistant, resulting in less off-target events. While both have similar advantages, bridged ...Nature has evolved ways to prevent degradation of important cellular RNA molecules with post translational...-Cas9, for use in applications like siRNA and antisense oligonucleotides. Some of these synthetic modifications...Frontiers in Genome Editing). This is particularly important in experiments in which viability is being compromised...technology is that most expression systems are constantly “on”. There is no mechanism to turn cutting off...
Showing: 581 - 600 of 1084 results