Plasmids 101: Gateway Cloning
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Blog Post
... clones. The entry clone and destination vector carry different antibiotic resistance markers (indicated...
We narrowed to 644 results for: CAR
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Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post... The best option in such instances is palliative care, which helps patients manage the pain and discomfort... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...Wang, Haoyi, et al. "One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...postdoctoral fellow at the University of North Carolina at Chapel Hill. Under the co-mentorship Blossom... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...something fascinating. Behavior is based primarily on careful observation of what an animal does naturally so... -
Plasmids 101: Control Plasmids
TypeBlog Post... are many, many different types of experiments carried out by scientists every day. Although the designs... -
Tips for Using FRET in Your Experiments
TypeBlog Post...and cannot be used for dynamic measurements. Extra care should be taken so as not to destroy the donor molecule... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...typically a tiny fraction of what was actually carried out. While no one is suggesting all data be stored... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...active and on all the time while others are more carefully controlled. Regulated promoters might act only... -
Handling Plasmids from Addgene - Purifying Plasmid DNA
TypeProtocol... g for 30 sec. Pour off the supernatant, being careful not to disturb the bacterial pellet. Resuspend ...Add 200 μL of Solution II and invert the tube carefully 5 times to mix the contents. The contents will...the supernatant into a new tube by pipetting or carefully pouring. Optional: Add 5 μL of 2 mg/mL RNase A...Pour out the supernatant in the sink. Pro-Tip Be careful, the pellet is harder to see and less well attached... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...away from its usual location, preventing it from carrying out its proper function (Figure 3). The Hehnly... -
CRISPR 101: Homology Directed Repair
TypeBlog Post... robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...the use of bright red fluorescent proteins (e.g. mScarlet) or infrared fluorescent proteins (Chernov et ... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...three pillars crashes, the lab's success and the careers of its researchers are on the line. With these... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...fluorophores, more are in development, including Carrot and Radish from the lab that developed Corn. Aptamers... -
Five Popular Model Organisms, Part 2
TypeBlog Post..., the rat is frequently used to study human cardiovascular disease, specifically stroke and hypertension... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post... G., Babina, M., Bertin, N., Burroughs, A. M., Carlisle, A. J., Daub, C. O., Detmar, M., Deviatiiarov,... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...fluorescence signal intensity. However, you need to be careful when selecting the primary antibody host species... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...25167058. PubMed Central PMCID: PMC4412028. 5. Araya, Carlos L., and Douglas M. Fowler. "Deep mutational scanning... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...phosphoryl-modification; they are both much smaller, and carry only a single negative charge as opposed to a double...