We narrowed to 641 results for: CAR

1. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

   Type

   Blog Post

   ...postdoctoral fellow at the University of North Carolina at Chapel Hill. Under the co-mentorship Blossom...

2. Important Considerations in Optogenetics Behavioral Experiments

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   Blog Post

   ...something fascinating. Behavior is based primarily on careful observation of what an animal does naturally so...

3. Plasmids 101: Control Plasmids

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   Blog Post

   ... are many, many different types of experiments carried out by scientists every day. Although the designs...

4. Tips for Using FRET in Your Experiments

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   Blog Post

   ...and cannot be used for dynamic measurements. Extra care should be taken so as not to destroy the donor molecule...

5. Data Freedom: The Expansion of Data Sharing in Research Publications

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   Blog Post

   ...typically a tiny fraction of what was actually carried out. While no one is suggesting all data be stored...

6. Plasmids 101: The Promoter Region – Let's Go!

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   Blog Post

   ...active and on all the time while others are more carefully controlled. Regulated promoters might act only...

7. Handling Plasmids from Addgene - Purifying Plasmid DNA

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   Protocol

   ... g for 30 sec. Pour off the supernatant, being careful not to disturb the bacterial pellet. Resuspend ...Add 200 μL of Solution II and invert the tube carefully 5 times to mix the contents. The contents will...the supernatant into a new tube by pipetting or carefully pouring. Optional: Add 5 μL of 2 mg/mL RNase A...Pour out the supernatant in the sink. Pro-Tip Be careful, the pellet is harder to see and less well attached...

8. Antibody Validation Using the Indirect ELISA Method

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   Protocol

   ...Cap the bottle and invert several times to mix. Carefully remove the plate seal from the 96-well plate and...4 °C . Section 3: Primary antibody incubation Carefully remove the plate seal from the 96-well plate and...°C . Section 4: Secondary antibody incubation Carefully remove the plate seal from the 96-well plate and...or overnight at 4 °C . Section 5: TMB reaction Carefully remove the plate seal from the 96-well plate and...

9. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   Blog Post

   ...away from its usual location, preventing it from carrying out its proper function (Figure 3).  The Hehnly...

10. CRISPR 101: Homology Directed Repair

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    Blog Post

    ... robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA...

11. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post

    ... G., Babina, M., Bertin, N., Burroughs, A. M., Carlisle, A. J., Daub, C. O., Detmar, M., Deviatiiarov,...

12. Antibodies 101: Beyond Surface Labeling

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    Blog Post

    ...fluorescence signal intensity. However, you need to be careful when selecting the primary antibody host species...

13. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post

    ...fluorophores, more are in development, including Carrot and Radish from the lab that developed Corn. Aptamers...

14. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post

    ...25167058. PubMed Central PMCID: PMC4412028. 5. Araya, Carlos L., and Douglas M. Fowler. "Deep mutational scanning...

15. Using Phosphoserine to Study Protein Phosphorylation

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    Blog Post

    ...phosphoryl-modification; they are both much smaller, and carry only a single negative charge as opposed to a double...

16. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post

    ...the use of bright red fluorescent proteins (e.g. mScarlet) or infrared fluorescent proteins (Chernov et ...

17. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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    Blog Post

    ...three pillars crashes, the lab's success and the careers of its researchers are on the line. With these...

18. Five Popular Model Organisms, Part 2

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    Blog Post

    ..., the rat is frequently used to study human cardiovascular disease, specifically stroke and hypertension...

19. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post

    ...including dementia, gut disorders, nerve damage, and cardiac disease. This increased disease risk is attributed...

20. Antibodies 101: The Basics of Western Blotting

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    Blog Post

    ... the western blot. When planning a western, take care to include the proper positive and negative controls...