We narrowed to 649 results for: CHI

1. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   ...dengue for many years. Since more than a thousand children died of malaria today, let's not risk a potential...

2. Tips for Using BLAST to Verify Plasmids

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   ...sequences and may be the most comprehensive for searching. Timesaving Tip #1: If you know the species that...

3. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   ..., P., & Berkmen, M. (2012). SHuffle, a novel Escherichia coli protein expression strain capable of correctly...

4. Viral Vectors 101: The Retroviral Lifecycle

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   ...into the host cell’s genome and then use cell machinery for transcription and translation. Here, we will...

5. DNA Purification Without a Kit

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   ...method from the Bishop Lab at the University of Chicago doesn’t use actual milk to purify DNA, but rather...

6. Transferable Skills Guide: Identifying Your Transferable Skills

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   ...technical expertise in a specific field. While searching for jobs and making connections, avoid falling...

7. MXS Chaining

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   ... 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic assembly of DNA molecules...

8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...have been developed to recruit transcriptional machinery to dCas9 and its RNA directed binding site. These...

9. Negative Can Be Positive: Open AAV Data with Addgene

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   ...214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard,...

10. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ...the accessibility of your target epitope(s). To achieve these goals, researchers commonly use one of two...

11. Lentiviral Vector Uses and Overview

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    ...Second, Tat is eliminated through the addition of a chimeric 5’ LTR containing a Tat-independent promoter. ...

12. Cpf1: A New Tool for CRISPR Genome Editing

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    ...location and GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function,...

13. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    ...Addgenies from wasting hours of valuable time searching for samples and keeps us from discarding tubes...

14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ...at the center of the structure, shielded from quenching by aqueous solvent. This tightly-packed structure...

15. Hot Plasmids: Fall 2024

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    ... abscessus either simultaneously or stepwise, achieving mutation efficiencies of up to about 9% of colonies...

16. How to Be an Excellent Trainee

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    ...maybe you would like to take pictures/videos of a machine set up while your mentor is demonstrating it. Requests...

17. Viral Vectors 101: Preparing Pooled Libraries

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    ...polyethyenimine transfection protocol. I also recommend searching PubMed for any publications associated with the...

18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...disrupt a catalytic residue. Good news! You can achieve your precise edit without even making a break.  ...

19. New CRISPR Tools: Cas7-11 and PASTE

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    ...system wasn’t dependent on the cell’s intrinsic machinery being already activated to work. (It was also ...

20. Antibodies 101: Immunoprecipitation

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    ...studying epigenetics routinely use the chromatin IP (ChIP) technique to identify DNA binding proteins involved...