We narrowed to 770 results for: Dos;

1. All in a Twist: dsRNA

   Type

   Blog Post

   ...produced dsRNA rarely reach this threshold and thus do not typically activate an inflammatory response. ...

2. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

   Type

   Blog Post

   ...interest into a bacterial cell or a host’s genome. To do this scientists commonly use phagemids, a DNA cloning...

3. FlipGFP, a novel fluorescence protease reporter to study apoptosis

   Type

   Blog Post

   ...spatio-temporal dynamics of apoptosis in living animals. To do this they modified a different self-assembling split...

4. Antibodies 101: Polyclonal Antibodies

   Type

   Blog Post

   ...strength of staining and background noise. If you do use different lots of your polyclonal antibody, you...

5. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   ...sensors in terms of response and brightness. They also do not spectrally separate from green sensors and blue-light-activated.... The dosing required to achieve these efficiencies was also between 1013 - 1014 vg/kg, a dosage comparable...

6. A Quick Guide to a Career in Software Product Management

   Type

   Blog Post

   ...go to grad school. But, I didn’t know what else to do. Fortunately, the head of software product management...

7. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   ...biology circuits. What is a molecular biologist to do? Let’s take a look at how plasmids recently deposited...JChristopher Anderson. "Rapid optimization of gene dosage in E. coli using DIAL strains." Journal of biological...

8. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   ...we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment? The three years...

9. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

   Type

   Blog Post

   ...communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural cells, ion-based...

10. CasPEDIA: A Functional Classification of Cas Enzymes

    Type

    Blog Post

    ...resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: ...

11. Summer SciComm: Preprints

    Type

    Blog Post

    ...broader community, and preprints are a great way to do that. We are happy to receive deposits of plasmids...

12. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    ...sequences to visually check for homology and mismatches. Do you have your own best sequencing practices or maybe...

13. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    ...and Validation Group and says that she decided to do a post-doc at Novartis because she wanted first-hand...

14. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    ... ends better substrates for ligation. Although we do not describe these steps here, the processing of ...

15. Plasmids 101: Cre-lox

    Type

    Blog Post

    ...Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

16. Addgene: The First Twenty Years

    Type

    Blog Post

    ...invaluable resource. I really appreciate the work you do to make sharing reagents and protocols so easy!” ...

17. Antibodies 101: Conjugation

    Type

    Blog Post

    ... unconjugated version, but if you find yourself doing your own conjugation, it is important to keep in...

18. Human Germline Editing Using CRISPR

    Type

    Blog Post

    ...diagnosis (PGD) screens embryos to find those that do not carry a given dominant disease-causing gene, ...

19. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    ...: A New Tool for CRISPR Genome Editing Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in...

20. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    ...References: Monticelli, M., Ferro, T., Jaeken, J., dos Reis Ferreira, V. & Videira, P. A. Immunological ...