Viral Vectors 101: Pseudotyping
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...find tables and information on different viral membranes, tropism, and advantages and disadvantages:
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Plasmids 101: Plasmid Incompatibility
TypeBlog Post...resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman et al., 2017)... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog Post...Remember the game show “The $25,000 Pyramid” where one player tries to get the other to guess a category... -
Transferable Skills Guide: Career Planning Resources
TypeBlog Post...clearly a good idea to be diligent in your research, remember that you will not be a graduate student or postdoc... -
Career Insights: Technical Support Specialist
TypeBlog Post...the larger biotech companies), and as far as I remember, it was the first time that I was absolutely convinced... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post... on the expertise of the senior leadership and members of their consulting staff. Who would enjoy healthcare... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...., Rubinstein, M. “LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis... -
Plasmids 101: Control Plasmids
TypeBlog Post...difference and is it significant? Vaux DL, et al. EMBO Rep. Apr; 13(4): 291–296. (2012). PubMed Central... -
Tips for Using FRET in Your Experiments
TypeBlog Post...Applications: An EAMNET Teaching Module http://www.embl.de/eamnet/downloads/modules/FRET_teaching_module.pdf... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...excellent efficiency using a commercial isothermal assembly master mix. Introduction of the strong transcriptional... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...luck with any experiments involving IDLVs. And remember: You can never trust a lentivirus with secrets... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post...running your analysis. In this case, I and other lab members had worked with lymphocytes before, so I knew where... -
Transferable Skills Guide: Time Management
TypeBlog Post...physically cross things out (it just feels good) and I remember things better when I write them down. Some people... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog Post...deliver these components to your target cells. Remember, efficient delivery of CRISPR components is essential... -
Evolution of Lab Techniques
TypeBlog Post...smaller pieces, run on a gel, and transferred to a membrane for analysis through hybridization of a radioactively... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...Gene-editing summit supports some research in human embryos. Nature. 2015 Dec 3. doi:10.1038/nature.2015.18947... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...Attribution License. Within the nervous system, remember that some serotypes can be transported retrogradely... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...this might be frozen or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...control process. Since MiSeq involves sequence assembly from short reads (<300 bp) which cannot distinguish... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...recommend using overlap extension PCR and Gibson assembly to piece together the registers. All parts used...