We narrowed to 638 results for: Erf

1. Hot Plasmids Spring 2024

   Type

   Blog Post

   ...Ace2N-mNeon also led to similar improvements in performance.      Figure 4: Structure of Voltron...

2. Tips for Writing a Good Cover Letter

   Type

   Blog Post

   ...for Scientists Series Get Tips on Finding Your Perfect Job after Graduation Additional Resources Hamilton...

3. Deciphering the Mysteries of Behavior with Viral Vectors

   Type

   Blog Post

   ...discussed some of the general considerations for performing behavioral tests, but now we’ll focus on how ...

4. Plasmids 101: Codon usage bias

   Type

   Blog Post

   ... in your expression host of choice, you should perform a functional assay to make sure your protein isn...

5. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   ...identified (Hart et al, Bassik et al). The library performed well in both screens, with the growth screen having...

6. CRISPR 101: Targeting RNA with Cas13a (C2c2)

   Type

   Blog Post

   ...from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian...

7. 5 Reasons to Use Reddit for Science Communication

   Type

   Blog Post

   ...publically about your work (you can find my tips for a perfect science talk here). Finally, my main content organizer...

8. Tips for CRISPR Gene Editing in Mice

   Type

   Blog Post

   ...generate your pCAG-EGXXFP plasmid? They are the perfect primer sets to use to genotype your pups. A simple...

9. The Challenges of Cell Culture

   Type

   Blog Post

   ...making it easier for comparable studies to be performed on a cell line even decades after it is initially...

10. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    ...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...

11. Important Considerations in Optogenetics Behavioral Experiments

    Type

    Blog Post

    ...in the laboratory mouse, most optogenetics is performed in this species. However, transgenic rat technology...

12. Plasmids 101: E. coli Strains for Protein Expression

    Type

    Blog Post

    ... expression from high-copy number plasmids and powerful promoters will greatly exceed that of any native...

13. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    ...a good place to start when trying to pick your perfect promoter! Eukaryotic Promoters   Promoter   ...

14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type

    Blog Post

    ...recruiting proteins to the cell membrane isn’t powerful enough, what about altering the chemical composition...

15. Tips for Getting a Faculty Position

    Type

    Blog Post

    ...weeds. Check out Joanne Kamens's "10 Steps to a Perfect Science Talk." 4. The chalk talk, simple in principle...

16. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    ...targeted-break technology coupled with HDR is a powerful site-specific knock-in tool that can be harnessed...

17. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type

    Blog Post

    ...notes etc. Detailed records about every action performed in the lab (user, date, time, action) are required...

18. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    ...cell types, now you know that AAVs can provide a powerful boost to genome editing experiments. We’re excited...

19. Transferable Skills Guide: Time Management

    Type

    Blog Post

    ...quick and useful tasks will swiftly give you the wonderful feeling of accomplishment while the longer tasks...

20. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    ...form of (hopefully constructive) conflict. As you perform the experiments to address a reviewer’s concerns...