We narrowed to 622 results for: Erf

...ease of use, 2) time savings, and 3) ability to perform single site or multi-site saturation mutagenesis...

...tagged protein because of its size. This type of interference is known as steric hindrance. For instance, ...

... intestinal crypt, and since then has become a powerful tool for understanding cell proliferation. Inducible...

...form of (hopefully constructive) conflict. As you perform the experiments to address a reviewer’s concerns...

...integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the synthetic...

...recruiting proteins to the cell membrane isn’t powerful enough, what about altering the chemical composition...

...weeds. Check out Joanne Kamens's "10 Steps to a Perfect Science Talk." 4. The chalk talk, simple in principle...

...targeted-break technology coupled with HDR is a powerful site-specific knock-in tool that can be harnessed...

...functional scFvs; however, some individual scFvs performing better in one configuration than the other (Sandomenico...

...relatively small, water soluble molecule that does not interfere with the macromolecules it is added to. Biotin...

...generate non-phosphorylated recombinant protein and perform in vitro kinase reactions. Often, however, researchers...

...upon the incorporation of a ddNTP. Researchers perform four different reactions, one for each radiolabelled...

... restriction site for linearization. This is a perfect case for the use of Gibson assembly combined with...

...of the  Brainbow multicolor labeling system - a perfect example of the continued utility of Cre-lox. Check...

...gene of interest, painstakingly cloned into the perfect empty backbone, made sure to add the right tags...

... Or is it teamwork that brings out your best performance and learning? Everyone’s different so give some...

... al., 2018). The small size of HiBiT reduces interference with the native function of tagged proteins ...

...Michael, S., Kuleshova, E., Ojala, D., Huang, C.-C., Gerfen, C. R., Schiller, J., Dudman, J. T., Hantman, A...

...first amount that looks effective. Ultimately, the perfect “high titer” is one that optimizes gene expression...

...polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases." Current...