Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,080 results for: IND

Showing: 581 - 600 of 1080 results

  1. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...Otherwise we will all have to wait a lot longer for individual labs to come up with discoveries on their own...cells under drug treatment.” Stegmeier especially finds the value in the wealth of resources on Addgene’...based on that experience, soon directed himself to finding “immunological and virological solutions to crises... efforts in gene therapy either through direct industry collaborations, grants, or setting up commercial...“The continuous creativity that Addgene takes to find ways to disrupt these inefficiencies is pretty remarkable...

  2. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type
    Blog Post
    ...Engineering Resources on Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory...condition known to the plant community as ‘plant blindness’ - the tendency for students to be more interested...and ordering platform soon to be released. For individuals interested in ordering from ABRC for the first...organizations such as the Center for Science and Industry (COSI) and the Franklin Park Conservatory. Do ...

  3. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...The stress of finding a ‘good’ antibody is something we’ve all experienced. Finding an antibody that ...tags A number of epitope tags exist – all with individual optimizations and specific monoclonal antibodies...high success rates across all applications. You can find FLAG antibodies on Addgene’s catalog including a...some proteins. Protein folding issues, steric hindrances, and destabilization can be brought about by ...

  4. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...and the plasmids, technologies, and researchers behind them. As part of our thanks to you for being a ...Chari Cortez explains some of the basic science behind homology directed repair (HDR) and how it’s used...experiments and tired of having to PCR many colonies to find a successful clone? Addgenie Jessica Welch explains...Chapel Hill explain some of the molecular gymnastics behind DNA repair by non-homologous end joining (NHEJ)...Career Coaching for Scientists: Why and Where Do I Find One? If you need help making a transition into ...

  5. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...of supplying a DNA construct, they could simply induce cells to take up protein directly? It’s not a new...delivery. D’Astolfo et al. coined the term iTOP (induced transduction by osmocytosis and propanebetaine)...showed up to 70% resistance to diphtheria toxin, indicating a 70% frequency of biallelic mutations. Importantly...Importantly, hESCs retained their stemness, indicating that iTOP may be valuable for CRISPR/Cas9 stem cell...674-690. doi:10.1016/j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and...

  6. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...clinical; can we find ways to treat patients? Over a quarter of a million people have some kind of neural implant...instead of blue, which will allow researchers to independently control the activity of two populations of neurons... of progress. I would say we still would like to find ways to make these tools more selective for different...link in the Addgene Alerts box. References: Independent Optical Excitation of Distinct Neural Populations...

  7. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ... but with the names reversed, meaning that the findings of the study were also reversed. The third group...participants read an abstract for a study with findings that challenged their beliefs, they were much ...catchall term for a range of neat little tricks our minds perform to avoid cognitive dissonance (Kunda, 1990...gained from that research remains inaccessible, behind paywalls and in technical jargon that even scientists...References Drummond, C., & Fischhoff, B. (2017). Individuals with greater science literacy and education have...

  8. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...strategy that would independently generate multiple gRNAs for nCas9-BE, or any other kinds of CRISPR tools...expression of the array. After expression, the individual gRNAs are released by endogenous tRNA processing...which is followed by tRNA processing to release individual gRNAs for gene editing. They named this new array...-target editing and do not cause higher Cas9-independent off-target editing in comparison with single-...

  9. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...collaborator for a specific task, Crowdfight will: 1) Find an expert with the right skill; 2) Confirm the expert...been essential to push certain projects. The idea behind the platform was simple: any person working on ... collaborations are limited by the difficulty to find the right expert and the lack of credit for small...researchers who believe in making science less of an individual effort and more of a collective construction....research. It’s completely free, of course. You can find more information about us and what we do in our ...

  10. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ... proteins (FPs) have become one of the most indispensable tools in modern biological research.  Any microscopist...proteins when missing, or edit inaccuracies as they find them (anyone can register with a valid email address...FPbase The most obvious use case for FPbase is finding information on a particular FP; this can be done...600nm?  Simply input these parameters and you’ll find matching FP suggestions. If you’re not sure exactly...Addgene.org Browse all fluorescent protein plasmids Find plasmids for FRET Check out the Michael Davidson...

  11. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...Libraries Resources at Addgene.org Find pooled libraries at Addgene Find basic lab protocols at Addgene ...preparing DNA and generating/replenishing kits, is to find new ways to make lab processes more efficient. Being... now know what to expect at a workshop and what kinds of questions to ask myself when I am bombarded with...information I’ll be learning relate to me as an individual and Addgene as a company? What’s the key thing...

  12. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...nucleic acid sequences that bind to the small molecule, DFHBI-1T to induce its fluorescence. They found...protein (dCas9) is well known for its ability to bind DNA targets without changing them. Thus, it has ...genomic loci brightly, give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma...Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR guide...

  13. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...shift can be prevented. Keep this information in mind if you’re planning on shipping something on dry ...medium, which is buffered and contains the pH indicator phenol red. We froze samples in a -80°C freezer... the samples were at the control pH of 7.4 as indicated by color (data not shown). What we did next: Venting... at -80°C. We then thawed the tubes after the indicated incubation periods, and measured the pH. We found...Resources on Addgene.org Check out the Viral Service Find Virus Protocols Browse Our Viral Vectors ...

  14. Science Career Options

    Type
    Blog Post
    ...opportunities available to those who are scientifically minded! Listen to Biotech Founder Michael Koeris' Journey...in-depth research is applicable to a wide range of industries and is a highly desirable quality sought after... are self organised, reliable, and can work independently. Practical outdoor skills are needed in a variety...currently in the position. Questions you’ll want to find answers to include: Will you be sitting at a desk... posts for more specifics on some careers and to find advice on crafting the perfect application. Additional...

  15. The MTA at Addgene

    Type
    Blog Post
    ...you ask? Well, organizations used to negotiate individual MTAs every time they wanted to share materials...Addgene — basically, instead of having to sign individual MTAs for each request, the depositing organization...bit confusing, and it’s certainly frustrating to find out your plasmid hasn’t shipped because a form needs...have the option of distributing under Addgene’s Industry MTA (IMTA), which allows the plasmids to be distributed...distributed for research use only to industry-based scientists. Some organizations choose to share their...

  16. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...common antimicrobial agent added to buffers, can bind to amine groups present in some conjugates, blocking...conjugates, or signaling molecules! Please keep in mind that this is just a brief introduction to the wide... if it ain’t broke, don’t fix it. HRP is easy to find, simple to use, not sensitive to light in its inactive.... But PE can be very helpful if you're trying to find a relatively rare protein in an experiment with ...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene...

  17. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral prep here Control AAV Control...presence of GFP to form a functional Cre enzyme that induces recombination of lox sites. This system takes advantage...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors...

  18. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...milestone in a scientist's career is mediated by some kind of communication to another person.  In an interview...specialized franchise workshops throughout the year.  Find workshops in your area here: https://comscicon.com...out of our workshops. If you're having trouble finding science communication opportunities in your area...do, let us know!  To get in touch with ComSciCon, find us on Twitter, Facebook, Instragram, or send an ...works as a data scientist in the entertainment industry.       Additional resources on the Addgene Blog...

  19. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...regulation is complex and influences--directly or indirectly--many other areas of biological research. If ...field who have deposited with Addgene, but you’ll find many great tools by clicking on the links below ...below pull up lists of plasmids containing the indicated gene, and you can subscribe to these pages to ...to pages containing lists of plasmids from the indicated publications. You can request all of these plasmids...956-972.e23 Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after...

  20. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ... to find protocols that require reagents which aren’t broadly or easily available, so you may find yourself...learn more about your protein of interest, you may find the available amino acids a bit…limiting. It may...type, and growth temperature) may need to be individually optimized for each of the three factors: the...trade-offs. This balance can be extremely tricky to find: each of the factors can negatively impact both ...
Showing: 581 - 600 of 1080 results