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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 937 results for: Ina;

Showing: 581 - 600 of 937 results

  1. Lab Automation at Addgene

    Type
    Blog Post
    ...is ideal because it eliminates the potential of human error or cross contamination between samples. With... Shannon Rinaldi...

  2. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...immunogenic profile. AAV was first discovered as a contaminant of adenovirus preparations. It is an icosahedral...untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the human gene... responses to adeno-associated virus and its recombinant vectors." Gene therapy 10.11 (2003): 964-976....

  3. Tips for Technical Support Calls

    Type
    Blog Post
    ...majority of our plasmids and pooled libraries were originally described in a peer-reviewed scientific publication... our thousands of depositors. That means their original articles are your best bet to figure out how to...asking to avoid unrealistic expectations. And finally, provide feedback. If you are unsatisfied with ...

  4. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...PCR product that comes from amplification of the original plasmid which can over-inflate results. A packing...vectors carrying fluorescent markers are used as it eliminates the need for antibody staining. Check Out Our...into a target cell’s genome. This approach can discriminate multiple integration events, reducing the possibility...

  5. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...researchers to use. Although we haven’t yet made our final target decisions, we intend to focus on motor neuron... a new project at a makerspace is finding the financial resources to pursue it. Since makerspaces are ...equipment for new uses. We’ll probably never use the Illumina GAIIx we recently acquired  for sequencing again...

  6. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...members all say the same thing and one panel member dominates the discussion? Or maybe the one that goes way...front of a crowd. The moderator will then develop a final list of questions for the actual event, based on...

  7. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...generated info for your own purposes, or delete the originals if desired. It works as a whole or in part. For...ranging between 0 and 1. The cutting frequency determination (CFD score) is used to measure specificity of...re-binding. Lastly, the sequence inserted by HDR may inadvertently re-create a match for the active guide. With...

  8. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ... an approach called Simultaneous Tracking of Recombinase Activation and Clonal Kinetics (STRACK) to study...epithelial cells, liver, CNS (limited penetration), retina, hematopoietic cells, and immune cells. B) AAV11...postsynaptic AMPA receptor and is endocytosed, the Cre recombinase payload activates a floxed reporter gene (genetically...

  9. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...increased sensitivity allows for a more confident determination of copy number, albeit within a range of RQ ...trends? How confident do you need to be in your determination to move forward with your experiment?  Complexity...real-time polymerase chain reaction methods for the determination of amplification of Plasmodium falciparum multidrug...

  10. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...the end product of PCR temperature cycling. The final PCR product is often visualized on a diagnostic ... dNTPs and other reagents to run low during the final cycles which would slow or stop PCR amplification...statistics to determine the concentration of the original template DNA in the sample.  Addgene currently...

  11. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...Figure 1). Through this method, we found key determinants of prime editing efficiency such as the length....0 while accounting for some of the inherent inaccuracies of computational prediction models. In addition...efficiencies using sequence features and DNA repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org...

  12. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...2014 to 2023. As you can see, nucleases, the original CRISPR tool, remain the most popular deposited...Read our recent publication, “The Expanding Dissemination and Distribution Patterns of Diverse CRISPR ..., R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution Patterns of Diverse CRISPR ...

  13. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...don’t get done if there is not a clear deadline. Finally check-in regularly, make yourself available, and...that one on one discussion can be confidential. Finally, provide timely feedback. We often associate feedback...manner. If someone’s performance or behavior is inadequate, you need to address it as soon as possible for...

  14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...active during M and early S phases, when HDR is inactive, and the balance of NHEJ, HDR, and MMEJ repair...longer the homology, the more efficient the recombination, but with longer homology arms comes more time...which has been difficult to modify due to NHEJ’s dominance over HDR in this species. As CRISPR technology...

  15. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...scientist. When our elementary school needed a new coordinator for the annual Math & Science Night, I took the...It’s through the PTA that I recently started coordinating the annual Math & Science Night, a chance for...contacting our school district’s science curriculum coordinator with minimal luck, but eventually the 8th grade...

  17. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...T. R. (2015). Stability and compatibility of recombinant adeno-associated virus under conditions commonly...Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct...Identification of factors that contribute to recombinant AAV2 particle aggregation and methods to prevent...

  18. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...for some applications, the benefit it offers is marginal. Fortunately, amp can easily be replaced with ...NPTII, facilitates phosphorylation of kan, which inactivates it. The NPTII gene confers resistance to several.../1475-2859-11-56 Trastoy, M. O., Defais, M., & Larminat, F. (2005). Resistance to the antibiotic Zeocin...

  19. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...labs and institutions. For example, search for “Melina Fan” and you’ll be able to go directly to a page...In this example we’re going to be looking for recombinant monoclonal antibody plasmids from the James Trimmer... ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed for other uses....

  20. Tips for arabidopsis transformation

    Type
    Blog Post
    ...are binary gateway vectors and can be used for promoter swapping and generating N and C terminally tagged...4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation ...
Showing: 581 - 600 of 937 results