We narrowed to 1,039 results for: LAS

...different cellular environments such as retina or glioblastoma). They also found that exo-AAVs were more resistant...Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving iodixanol gradients...brain tumor stromal cells in a mouse model of glioblastoma (Volak et al. 2018). The structure of exosome...

... to our volunteer list. We then collected and classified their responses, and used that information to... favors from becoming starting points for long-lasting partnerships Join us! We are a community of researchers...Resources on Addgene.org Browse Addgene's COVID-19 plasmids and resources ...

...Addgene.org Browse all fluorescent protein plasmids Find plasmids for FRET Check out the Michael Davidson...Looking for a bright, monomeric FP published in the last few years with an emission peak around 600nm?  Simply...

...Addgene Wesbite Find Reprogramming Plasmids Find Other Hot Plasmid Tools Visit Our Stem Cell Pages ...gratifying to see that Addgene has ensured that plasmid acquisition is not a rate-limiting step in stem...

...enough. For example, if we need to develop a new plasmid collection page, we certainly don't expect that...design every icon, curate all of the appropriate plasmids, and code the entire page. That would be entirely...Addgene Website Are You a Molecular Biologist? Find Plasmids for Your Research Here!        ...

...that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website,...mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists ...Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

...Flash quiz! What pops into your head when you hear the phrase “action potential?” Firing neurons are ...through gap junction proteins which connect the cytoplasm of two cells) can also affect patterning and in...GEVI features increased brightness and improved plasma membrane specificity. Stable Step Function Opsin...

... give as a journal club to my molecular biology class. A Cell paper. With six figures. Averaging ten panels... assignment was clear: present a paper to our classmates so they could understand it. When my mind went...incidents above–and the dumpster noises were just last week!  Nerves It’s completely normal to be anxious...

... tag plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing...from Addgene More Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies...

...   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s primary difference from Cas9.... Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell, 163(3): 759-771 (2015...More resources on the Addgene Website CRISPR Plasmids and Resources CRISPR Guide  ...

...to antibodies. Instead, they are derived from a class of binding proteins known as ankyrin repeat proteins...Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs...available from AddgeneBrowse our recombinant antibody plasmid collection References Grumezescu, A. M. (2018)....

...double-membraned structures containing undigested cytoplasmic contents. Autophagosomes that have fused with... example of what fluorescence looks like for cytoplasmic mitochondrial Rosella with and without autophagy... Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent Protein Guide: Biosensors...

...Chris Voigt for speaking with us! Find the Plasmids: Plasmids for multi-input CRISPR/Cas9 genetic circuits... required to get the function that you want. The last few years, we’ve really been making tons of parts...

...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...CRISPR   Resources on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible...light-inducible Cas9 activity? Check out these these plasmids Catch Up on Your CRISPR Background with Our Guide Pages...

...the Quality Control team, I help make sure the plasmids that go out our doors are correct. Now that I...Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community college when she took her...interest in data science,” which she’s taking some classes on and hoping to pursue. The choice to not go to...

... instead that it is noninvasive and has longer-lasting effects. Building your chemogenetic system To set...raising the threshold for signaling. The two main classes of receptors currently used in chemogenetics are...viral preps for your research Find chemogenetics plasmids for your research ...

...drawbacks to this approach: TET proteins are not demethylases; they are dioxygenases that oxidize 5-methylcytosine...achieved by dCas9 alone, without TET (or any demethylase) fusion. This may seem counterintuitive: how ...achieved using lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple...

...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts...the full amount of time, every time. Imaging The last step in a western blot tends to be the most straightforward...

...large-scale prime editing experiments come into play. Nicolas Mathis received his PhD from Gerald Schwank's lab...sciadv.aax9249 Koeppel, J., Weller, J., Peets, E. M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante....1038/s41587-022-01613-7 Mathis, N., Allam, A., Tálas, A., Kissling, L., Benvenuto, E., Schmidheini, L...

...Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis...amplicon can be used in molecular cloning to create a plasmid of interest, for detecting the presence of an insert...