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We narrowed to 1,035 results for: LAS

Showing: 581 - 600 of 1035 results

  1. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...Modify your plasmid MCS or add short sequences using oligo overlap cloning.... Protocols Plasmid Modification...Modification by Annealed Oligo Cloning Plasmid Modification by Annealed Oligo Cloning You may also like... Restriction...Restriction Digest of Plasmid DNA DNA Ligation Bacterial Transformation Summary Oligo overlap cloning can...anytime you need to add a short stretch of DNA to a plasmid, such as: Changing a Multiple Cloning Site (MCS...

  2. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Will...sites to your recipient plasmid : You can modify the MCS of your recipient plasmid using Annealed-oligo ...material. We recommend 1.5-2μg of donor plasmid and 1μg of recipient plasmid. It is also critical that as much...re-circularization of the recipient plasmid. You should treat your digested recipient plasmid with a phosphatase prior...ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone ... on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence...

  3. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... instead that it is noninvasive and has longer-lasting effects. Building your chemogenetic system To set...raising the threshold for signaling. The two main classes of receptors currently used in chemogenetics are...viral preps for your research Find chemogenetics plasmids for your research ...

  4. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...the Quality Control team, I help make sure the plasmids that go out our doors are correct. Now that I...Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community college when she took her...interest in data science,” which she’s taking some classes on and hoping to pursue. The choice to not go to...

  5. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...drawbacks to this approach: TET proteins are not demethylases; they are dioxygenases that oxidize 5-methylcytosine...achieved by dCas9 alone, without TET (or any demethylase) fusion. This may seem counterintuitive: how ...achieved using lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple...

  6. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...CRISPR   Resources on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible...light-inducible Cas9 activity? Check out these these plasmids Catch Up on Your CRISPR Background with Our Guide Pages...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts...the full amount of time, every time. Imaging The last step in a western blot tends to be the most straightforward...

  8. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...large-scale prime editing experiments come into play. Nicolas Mathis received his PhD from Gerald Schwank's lab...sciadv.aax9249 Koeppel, J., Weller, J., Peets, E. M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante....1038/s41587-022-01613-7 Mathis, N., Allam, A., Tálas, A., Kissling, L., Benvenuto, E., Schmidheini, L...

  9. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis...amplicon can be used in molecular cloning to create a plasmid of interest, for detecting the presence of an insert...

  11. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...neurogenesis, neuronal survival, and synaptic plasticity. Several studies have found reduced BDNF levels...prefrontal cortex Drd1 neurons produces rapid and long-lasting antidepressant effects." Nature communications ...Huijstee AN, Mansvelder HD. Glutamatergic synaptic plasticity in the mesocorticolimbic system in addiction....

  13. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...plans and in particular, that they all share the plasmids created under this programs via Addgene. New organisms...Cichlids are 2,000 fish species that evolved in the last 10,000 years (which is considered recent). They ...sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get transgenic animals. They...

  14. The Challenges of Cell Culture

    Type
    Blog Post
    ...profile problems surrounding cell culture over the last few decades some pharmacologists have tended to ... information in your lab book and on the actual flasks of cells in the incubator). This means any other...Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...

  15. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...varying between 10-50°C. Visit Addgene's COVID-19 Plasmids and Resources Many thanks to our guest bloggers...more about SHERLOCK and DETECTR Learn more about plasmids available for COVID-19 research Resources on ...in our CRISPR guide Browse our entire COVID-19 plasmid collection ...

  16. March for Science

    Type
    Blog Post
    ...-time, and have received a research grant in the last five years as polled in the American Association... funding for science has been decreasing for the last five years and the first federal budget request ...March for Science Website Locke, John, and Peter Laslett. Locke: Two treatises of government student edition...

  17. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...complicated procedures. Plasmid Cloning Protocols for constructing and analyzing your plasmids. Virus Protocols...Amplify CRISPR pooled-plasmid libraries Diagnostic Restriction Digest Verify a plasmid using restriction ... that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations...Bacterial Glycerol Stocks Store bacterial strains or plasmids for long term use Watch the Video! DNA Purification...spectrophotometer Restriction Digests Cut and analyze plasmid DNA with restriction enzymes Agarose Gel Electrophoresis... the Video! Sequence Analysis Verify important plasmid features using sequence analysis RNA Extraction...from tissues or cells without a commercial kit Plasmid Cloning Name Description (Link opens in a new window...

  18. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ... Protocols Restriction Digest of Plasmid DNA Restriction Digest of Plasmid DNA You ... quickly check the identity of a plasmid by diagnostic digest . Last Upload: Oct. 11th, 2016 Protocol ...methylation sensitive . Plasmids grown in Dam or Dcm methylase positive strains will be resistant...digest of plasmid DNA Protocols...You may also like... Recovering Plasmid DNA from Bacterial Culture Purifying DNA from an Agarose Gel DNA...chamber Pipetman Reagents Liquid DNA aliquot of your plasmid of interest (see below for recommend amounts) Appropriate...Procedure Select restriction enzymes to digest your plasmid. Pro-Tip To determine which restriction enzymes...

  19. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...experiments? In this post, we will discuss some broad classes of optogenetic tools, and what factors to consider...using optogenetic tools Once you’ve selected the class of tool appropriate for your experiment, you’ll ... https://doi.org/10.1038/nmeth.1808 Nagel, G., Szellas, T., Huhn, W., Kateriya, S., Adeishvili, N., Berthold...

  20. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...You’ve gotten the plasmid encoding your protein of interest from Addgene, transfected it into your target...from a variety of vendors and you may even find plasmids encoding antibodies from Addgene that you can ...cellular compartments such as the mitochondria, cytoplasm and nucleus.  We hope that this blog has provided...
Showing: 581 - 600 of 1035 results