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We narrowed to 876 results for: NSI;

Showing: 581 - 600 of 876 results

  1. Pipetting Protocol

    Type
    Protocol
    ...1 mL). When working in a laboratory, properly dispensing liquid ensures the accuracy of your experiment...experiment and any changes to the amount you are dispensing can negatively impact your experimental results. ...the liquid and avoid touching the pipette to the inside of the tube. Only the tip should be in the liquid...different tubes or into wells of a microplate, you may consider using a multichannel pipette. Please watch Addgene...

  2. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...increase activity and decrease the gRNA size by intensively remodelling the gRNA scaffold through five rounds...tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable of correcting...

  3. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...your network. If someone has connected with me, I consider them fair game for connecting to others.  Pro ...including and especially the hiring manager. This is considered intrusive and it’s still connecting with a stranger...

  4. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...Streak on them an E. coli  strain known to be sensitive to that antibiotic, and if the strain is growing...positive clone after these steps, you might want to reconsider your approach, and try a different cloning strategy...

  7. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...phosphorylated MEK1/2 levels, suggesting PP1/PP2A is responsible for this MEK1/2 negative feedback. 3. Studying...Silvia Carrasco, and Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in...

  9. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...toxin-antitoxin system encoded by the ccd operon, which is responsible for plasmid maintenance during cell division....E. coli do not contain the F plasmid (and are considered F-); however, there are a few popular lab strains...

  11. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...abundance before and after a treatment. The library consists of a pool of lentiviral vectors with roughly 73...populations, they found that similar barcodes consistently made up the majority of the resistant populations...

  12. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...scientists to confidently choose reliable and consistent antibodies for specific markers. The Screening...production methods    NeuroMab and others have an extensive library of monoclonal antibodies; Addgene collaborates...

  18. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...Special breeding cases Whether your mouse colony consists of genetically engineered mice with a single mutation...strains that have been crossed, there are many considerations for effectively breeding your mice. Because...

  19. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... crossing the blood-brain barrier.  Another consideration when picking ligands for chemogenetic studies...response you aim to manipulate. It is important to consider that the same receptor can elicit different responses...

  20. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...abundance of your targets isn’t too different, consider antibody affinity: stronger binding antibodies...membranes recommend re-wetting with methanol before rinsing with wash buffer, as dry PVDF won’t evenly re-absorb...
Showing: 581 - 600 of 876 results